TORTORA • FUNKE
• CASE
Microbiology
AN INTRODUCTION
EIGHTH EDITION
B.E Pruitt & Jane J. Stein
Chapter 8, part A
Microbial Genetics
Terminology
• Genetics
• Gene
Study of what genes are, how they
carry information, how information is
expressed, and how genes are
replicated
Segment of DNA that encodes a
functional product, usually a protein
Terminology
• Genome
All of the genetic
material in a cell
• Genomics Molecular study of
genomes
• Genotype
Genes of an
organism
• Phenotype Expression of the genes
E. coli
Figure 8.1a
Flow of Genetic Information
Figure 8.2
DNA
• Polymer of nucleotides:
adenine, thymine,
cytosine, guanine
• Double helix associated
with proteins
• "Backbone" is
deoxyribose-phosphate
• Strands held together by
hydrogen bonds
between AT and CG
• Strands are antiparallel
Figure 8.4
DNA
Figure 8.3
DNA
Figure 8.5
DNA
• DNA is copied by DNA polymerase
– In the 5  3 direction
– Initiated by an RNA primer
– Leading strand synthesized continuously
– Lagging strand synthesized discontinuously
– Okazaki fragments
– RNA primers are removed and Okazaki
fragments joined by a DNA polymerase and
DNA ligase
– See web sites
•
•
•
http://bioteach.ubc.ca/TeachingResources/MolecularBiology/DNAReplication.swf
http://www.ncc.gmu.edu/dna/repanim.htm
http://www.mun.ca/biology/scarr/3250_DNA_replication_&_transcription.htm
•
http://www.nobel.se/medicine/educational/dna/a/replication/replication_ani.html
•
http://www.nobel.se/medicine/educational/dna/a/replication/lagging_ani.html
DNA
Figure 8.6
DNA
• DNA replication is semiconservative
Figure 8.7
Transcription
• DNA is transcribed to make RNA (mRNA,
tRNA, and rRNA)
• Transcription begins when RNA polymerase
binds to the promotor sequence
• Transcription proceeds in the 5  3
direction
• Transcription stops when it reaches the
terminator sequence
• Web sites:
–
http://www.nobel.se/medicine/educational/dna/b/transcription/
Figure 8.8
RNA processing in Eukaryotes
Figure 8.12
Translation
• mRNA is
translated in
codons (3
nucleotides)
• Translation of
mRNA begins at
the start codon:
AUG
• Translation
ends at a STOP
codon: UAA,
Figure 8.2
Translation
Figure 8.9