9. (StI-BHEM) BIOCHEMISTRY - Medicinski Fakultet Univerzitet Novi

advertisement
9. (StI-BHEM) BIOCHEMISTRY
STUDY
PROGRAMME
DEPARTMENT
Integrated studies of dentistry
Departmen for biochemistry
NAME OF SUBJECT BIOCHEMISTRY
Compulsory
STATUS OF THE
SUBJECT
Condition none
Winter term (No.of the
Summer term (No.of the
No.of
No.of
Year of
lessons per week
lessons per week)
No.of tests
seminars
seminars
studies
seminara
POINTS
Lectures
Exercises
Lectures.
Exercises
first
3
3
2
7,0
Methods of conducting
Lectures for bigger and smaller groups using multimedia. Testing. Practical knowledgeteaching
conducting biochemical analysis and reading results.
GOA
L
PURPOSE
The goal of teaching biochemistry is to teach students to understand all biological processes in our body, to learn
about basic methods used in clinical chemistry as well as diagnostical means. To enable future doctors to use these
methods in right manner.
Knowledg Knowing basic chemical constituents of human body, general metabolical ways, understanding the
essence of many diseases and knowing specific biochemical processes of some organs and tissues.
e
Appropriate taking of biological material for biochemical analysis. Assessing reliability of some
biochemical methods and their use for diagnosis. Using results of biochemical analysis for diagnosis.
Skills
Examining metabolism the most important content of organism based on measures on biological
samples.proving basic laws of biochemistry based on laboratory methods.
CONTENT OF THE SUBJECT:: Theoretical teaching – methodical units
1. Introduction to biochemistry.
2. Water as biological solvent.
3. Aminoacids. Peptides..
4. Proteins – structure, qualities, classification. Hemoproteiesi – hemoglobin, mioglobin i citohroms.
5. Nuclear acids, bases, DNA, genetic code, RNA.
6. Phospolipides and biological membranes.
7. Enzymes-chemical nature,enzyme catalysis. Cinetics of enzyme reaction. Classification. Coenzymes and vitamins.
8. Chemical thermodynamics. Bioenergetics. Biological oxidation. Transformation of energy. ETS,ATP.
9. Metabolical ways. Digesting. Glicolysis. Krebs's cycle of lemon acid.
10. Digesting of proteins and absorption of aminoacids.
11. Digesting and absorption of lipids. Metabolism of lipoproteins.
12. Beta oxidation of fatty acids. Regulation of the metabolism.
13. Synthesis of DNA-replication. Synthesis of RNA-transcription. Synthesis of proteins.
14. Water and electrolytes.
15. Calcium and its importance.
16. Parathormon, D-hormone i kalcitonin.
17. Biochemistry of blood-blood plasma, coagulation of blood, biochemistry of eritrocites.
18. Biochemistry of connective tissue
19.Bone,dentin,cement and enamel.
20. Oral biochemistry.
.
21. Hormones. Mechanisms of action of steroid hormones. Hormones tireoideje. Adrenaline and norepinephrine. Hormones of the
pancreas. Corticosteroids. Male and female sexual hormones. Hormones and aden neurohipofize.
Practical teaching – methodical units
1.
2.
3.
4.
5.
6.
7.
8.
Introduction. The aim of exercises. Summary of teaching. Checking the reliability of biochemical methods
Photometry. Principles of Lambert-Beer's law. Ekstinkcioni and molar extinction coefficient. Blind test, and the standard
calibration curve. Kolorimetar and spectrophotometer. Application of Photometry. Checking the Lambert-Beer's law and
determining the concentration bromtimol blue.
Kolorimetric determining the concentration of inorganic phosphate.
Kolorimetric determination of protein. Methods for determining protein. Determination of protein concentration in serum biureten
reaction,
Isolation, fibrinogene method.
Polarimetrija. Determining a specific angle of turning the plane of polarized light and glucose concentration glukoze u urinu.
Quantitative determination of urea Berthelot method.
Qualitative analysis of bile color in the serum and urine.
9.
10.
11.
12.
13.
14.
Quantitative determination of chloride in serum.
Qualitative proving enzymes.
Enzymatic hydrolysis of starch-alpha amylase activity.
Principles of quantitative measurement of enzyme activity. Determination of alkaline phosphatase activity in function of time.
Proof of the existence izoenzima alkaline phosphatase.
Jonochanging chromatography of amino acids.
1. S. Marinkov, J. Borota: Medicinska biohemija, Radnički univerzitet ”Radivoj
Ćirpanov” Novi Sad, 2006.
2. J. Borota i saradnici: Praktikum medicinske biohemije i hemije, Medicinski fakultet,
READING
Novi Sad, 2006.
Additional
1. Z.Kovačević: Biohemija i molekularna biologija, Medicinski fakultet, Novi Sad, 2006.
Evaluation of students' work – No.of points per individual activity
Pre-exam obligations
Final exam
Total
Lectures
Exercises
Test
Seminar paper
The rest
Written
Oral
100
18
35
16
31
Compulsor
RECOMMANDED
y
List of teachers and assistents
Associate
Assistent
Lecturer
5
1.
2.
3.
4.
Prof dr Jela Borota
Doc. dr Katica Bajin Katić
Doc dr Karmen Stankov
Doc dr Ljiljana Andrijević
Prof. struk.
PhD
3
5.
6.
7.
8.
9.
Associate
prof.
Professor
Scientist
1
Asst. mr Mirjana Milošević-Tošić
Asst. dr Tatjana Ćebović
Asst. mr Jelena Stojčević-Maletić
Asst. mr Jasmina Katanić
Asst. mr Jovica Oros
Chief of department
Prof. dr Jela Borota ensuring
Download