Supplemental Instruction
Exam 2 Review
Kristin
3-5-13
1. To utilize energy, an animal cell must
a. immediately burn sugar
b. store it as fat
c. convert it to an intermediate
d. complete the electron transport chain
2. In adenosine triphosphate, energy is
stored
a. in the adenine nucleotide base
b. the ribose sugar
c. the first phosphate group
d. the third phosphate group
3. After ATP is used by a cell it is
a. disposed of as waste
b. recycled
c. completely broken down into
individual atoms
d. excreted from the cell
4. When delta G is released by a
chemical reaction (-), the overall process
is labeled as
a. favorable
b. exergonic
c. spontaneous
d. all of the above
5. When products are at a higher energy
state than reactants after a chemical
reaction, the process is
a. favorable
b. endergonic
c. exergonic
d. a and c
6. Combining the favorable process of
hydrogen moving down its
concentration gradient across an
electrochemical gradient and the
nonspontaneous process of bonding a
phosphate to an ADP is an example of
a. duplicating
b. coupling
c. catabolism
d. reductionism
7. When NAD+ is converted to NADH it
is being
a. reduced
b. oxidized
c. metabolized
d. synthesized
8. Thermodynamics cannot tell you
a. the kinetics of a reaction
b. the favorability of a reaction
c. the rate of a reaction
d. the mechanism of a reaction
e. a and c
9. The effect of an enzyme on a chemical
reaction
a. accelerates it
b. creates more product
c. creates an exergonic environment for
the reaction to take place in
d. all of the above
10. The relation between Km (binding
affinity of a substrate) and Vmax
(maximum velocity of a rxn) can be
summarized by which equation
a. Km=2*Vmax
b. Km=Vmax-2
c. Km =Vmax/2
d. Km =Vmax^2
11. A competitive inhibitor is designed
to seek out which spot on an enzyme
a. active site
b. tertiary site
c. primary site
d. two of the above are correct
12. An example of anabolism is
a. digesting food in your stomach
b. bonding AA in protein synthesis
c. burning fat for energy
d. ATPADP+Pi
13. T/F In a reaction involving a
competitive inhibitor, the reaction will
reach the same amount of products as if
it were uninhibited.
a. True
b. False
14. T/F Gylcolysis is an aerobic process.
a. True
b. False
15. According to endosymbiotic theory,
the outer membrane of the
mitochondria would have resulted from
a. the outer cellular membrane of the
ancestral aerobic prokaryotic symbiont
b. the inner membrane of the ancestral
aerobic prokaryotic symbiont
c. the cellular membrane of the ancestral
anaerobic host cell
d. it was created internally after
formation
16. Products of glycolysis include
a. pyruvate, NADH, and ATP
b. fructose biphosphate, NAD+, ATP
c. ATP, citric acid, phosphate
intermediate
d. Carbon dioxide, ATP, pyruvate
17. Energy intermediates involved in
cellular respiration include
a. NADH
b. ATP
c. FADH2
d. IDGAF
e. a,b,c
18. In the final step of cellular
respiration, on the mitochondrial
membrane, NADH does what
a. accepts electrons
b. donates electrons
c. attracts H+ions moving them across
the membrane
d. acts as the final electron acceptor
e. two of the above
19. Chlorophyll absorbs which lengths of
light
a. blue
b. green
c. red
d. a and c
e. all of the above
20. In the Hershey chase experiment,
which was found to have entered into
the E. Coli cell? Did this prove beyond a
reasonable doubt the genetic material?
a. Protein, yes.
b. Deoxyribonucleic acid, yes.
c. Deoxyribonucleic acid no.
d. Protein, no.
e. Guanine triphosphate, yes.
21. X-ray diffraction of a DNA fiber was
used to determine the
a. double helical shape and uniform
diameter
b. the antiparallel pattern and length
c. the model of transcription
d. the method of bacterial
transformation
22. Chargaff’s rule states that
a. the DNA replication is semiconservative
b. the sugar phosphate backbone is
positively charged
c. the ratio of purines: pyrimidines is
50:50
d. ratio of A:C equals that of G:T
23. During replication which property
of deoxyribonucleic acid leads to the
problem of a leading and lagging strand?
a. sugar phosphate backbone
b. complementary nucleotide bases
c. antiparallel nature of each side of the
strand
d. all of the above
24. Transcribe the RNA complement of
the following DNA sequence
5’- ATGAGCGACCTT-3’
a. 5’- TACTCGCTGGAA-3’
b. 5’- UACUCGCUGGAA-3’
c. 5’- AAGGTCGCTCAT-3’
d. 5’- AAGGUCGCUCAU-3’
25. The hydrogen bonds between
guaning and cytosine are (stronger or
weaker) than hydrogen bonds between
adenine and thymine.
26. Centrifugation separates materials
by ____________ and was used to
determine that DNA was _______________.
a. size, conservative
b. weight, dispersive
c. density, conservative
d. density, semi-conservative
e. size, semi-conservative
27. During DNA replication daughter
strands can only be synthesized from
(5’ 3’ or 3’ 5’)?
28. The enzyme DNA primase makes
what in the replication process a
a. DNA primer
b. RNA primer
c. DNA 5’ cap
d. RNA 5’ cap
29. In the model shown via media in
lecture, what is unique about the
replication process?
a. DNA Ligase synthesized the lagging
strand in the same direction as the
leading strand.
b. DNA helicase created two replication
forks on opposite sides of the replication
bubble
c. There was no polymerase chain
reaction
d. The DNA polymerase III on the
leading strand and the DNA pol. III on
the lagging strand were linked, creating
a loop.
e. There was nothing unique about it,
just replication as usual.
30. Which of the following
enzyme/function pairs is incorrectly
matched.
a. Ligase- bonds okazaki fragments
b. DNA Pol. I- Removes and replaces
primers
c. Topoisomerase- releaves helical strain
in the replication bubble.
d. SS binding proteins- prevent
hydrogen bonds between
complementary bases on separated
strands from reforming.
e. Telomerase- solves the 3’ overhang,
end of replication issue
31. Diagram the central dogma.
32. Operons control gene expression at
which step in protein synthesis?
a. protein folding
b. translation
c. helix separation
d. transcription
e. none of the above.
33. Place the following in the correct
order that they would appear on the Lac
Operon.
1. Termination sequence
2. Lac I gene
3. CAP binding site
4. Intron 2
5. Promoter
6. i promoter
7. Operator
8. Lac Z gene
9. Lac X gene
10. Intron 1
11. Lac Y gene
12. Start site
a. 3, 5, 2, 6, 8, 7, 12, 9, 1, 4, 11, 10
b. 2, 3, 7, 1, 12, 9, 10, 4, 6, 5, 8, 11
c. 6, 2, 3, 5, 7, 12, 9, 10, 11, 4, 8, 1
d. 6, 2, 5, 12, 9, 11, 4, 7, 8, 3, 10, 1
34. mRNA processing is unique to
eukaryotes and involves which of the
following.
a. 5’ ap
b. Poly-A tail on the 3’ end
c. splicing
d. transcripion level gene control
35. The presence of Lactose sugar
(induces, depresses) the Lac operon,
while glucose (induces, depresses) the
Lac operon.
36. Allolactose is a small molecule
created from_________ , and has what
effect on the Lac operon.
a. glucose, inducing
b. fructose, repressing
c. cAMP, repressing
d. lactose, inducing
37. Cyclic AMP (cAMP) is a small
molecule derived from _________ and has
a ___________ effect on the Lac operon.
a. Lactose, represses
b. Glucose, represses
c. Deoxyribonucleic acid, induces
d. Adenosine triphosphate, induces
e. both a and c depending on the
situation
38. An example of post-transcriptional
gene regulation is
a. alternative splicing
b. cellular mRNA storage
c. programmed protein lysis
d. termination inducers/repressors