Name:_______________________
OBJ 3.3.2
GENETIC ENGINEERING (Transgenic Organisms)
What is a TRANSGENIC
ORGANISM?
What is GENETICALLY
MODIFIED FOOD (GMO)?
TRANS =
GENIC =
ORGANISM =

It is an organism that has had genes __________________________ (or moved into) from a
different organism

Transgenic organisms are made from combining _______________________DNA

It is a crop or food that has been _________________________ modified, _____________________ or
__________________________.
What is SELECTIVE
BREEDING?

A type of _____________________________ engineering

Choosing organisms (plants, animals) with the best _____________________ (genes) and
______________________________________ them together to produce _____________________ (bigger,
stronger) offspring
CHECK POINT
1.What is a Transgenic Organism?
2.How are transgenic organisms made?
3.What is a GMO?
4.What is a real-life example of a GMO?
5.What is selective breeding?
6. What is a real-life example of selective breeding?
What is RECOMBINANT
DNA?

When __________________ from _____________ _____________________________ organisms come
together, the DNA becomes ___________________________________ ______________

ALSO KNOWN AS…
What does recombinant (recombine) mean?
Name:_______________________
OBJ 3.3.2
How do scientists create
DNA?

It’s all about using ____________________________!
What form is bacterial
DNA?
____________________________________________
What are the STEPS for
creating a Transgenic
Organism?
1. _____________________ the _____________________
2. Cut the bacterial DNA using _______________________________ ______________________
3. Remove and cut a ________________________ _______________________ from a different organisms
(foreign _______________)
4. Then _____________________ a piece of foreign (human, plant, animal) DNA back into the
___________________ using the enzyme _____________________.
5. __________________ the _____________________ back into the _______________________
6. Now, the bacteria will ____________________ the new __________ (in the plasmid DNA) to make
larger quantities of the _______________________ gene or trait.
ALL Bacteria are…
____________________________________________!!!!!!!
What are the
APPLICATIONS of
RECOMBINANT DNA?
SO…Every new bacteria will be making _______________ copies of the desired gene!
 Bacteria

–
Used to produce ______________________, antibiotics and _____________________
–
Used to clean up ____________ Spills!!
Plants
–

Made to be _________________ to herbicides and ___________________________
Animals
–
Cloning
–
Replacement of ________________ which cause disorders (aka _______________
__________________________)

Such as Cystic Fibrosis

Severe Combined Immunodeficiency
Name:_______________________
Example 1:
Example 2:
Example 3:
OBJ 3.3.2
Name:_______________________
OBJ 3.3.2
Genetic Engineering Video Guide:
http://www.youtube.com/watch?v=AEINuCL-5wc&feature=youtube_gdata_player
1. What does diabetic person need to stay regulated?
2. What kind of organism is this produced in?
3. What makes “You…You”?
4. When is an organism “Genetically Modified”?
5. How can you cut the desired enzyme out?
6. In bacteria, some DNA id found in circles. What is this called?
7. How are the circular pieces from #6 used?
8. How do you insert the desired gene into #6?
9. What is ligase? And what does it do for the gene?
10. What is then placed back into the bacteria?
11. Once #10 is completed, why is it called a “Genetically Modified Organism”?
12. What does the bacteria do after the step in #10 is completed?
Plasmids | Genetics | Biology
http://www.youtube.com/watch?v=GNMJBMtKKWU&feature=youtube_gdata_player
13. What 2 specific genes are mentioned throughout the video that are used for mass
production?