DEVELOPMENT OF H-SHAPED MICROFLUIDIC DEVICE WITH IMAGE DETECTION
AND ITS APPLICATION TO COLORIMETRIC DETECTION
Nanta Sreekeaw1,*, Prapin Wilairat2, Duangjai Nachapricha3, Rattikan Chantiwas1,#
1
Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of
Science, Mahidol University, Rama VI Rd, Bangkok 10400, Thailand
2
National Doping Control Center, Mahidol University, Bangkok 10400, Thailand
3
Flow Innovation-Research for Science and Technology Laboratories (FIRST Labs), Thailand
*e-mail: nanta.sreekeaw@gmail.com, #e-mail: rattikan.cha@mahidol.ac.th
Abstract
In this work, a microfluidic system using H-shaped microfluidic device with image
detection was developed. H-shaped microfluidic device has two inlets for introducing the
reagents which mix together at a merging point. The chemical reaction between iron (III)
ions and thiocyanate ions was employed as the test system with image detection to monitor
the red color of the product. The grayscale intensity was used as criteria to select the
optimum conditions for the microfluidic system and image detection. The appropriate
detection position on the microdevice was studied. In this study, RGB (Red, Green, Blue)
and grayscale intensity was used for image evaluation. Both RGB and grayscale intensity
were used to determine the iron content. Preliminary results using the RGB and grayscale
image analysis showed reasonably agreement with the amount of iron in iron supplement
sample as given on the label.
Acknowledgements:
This work is supported by the Center of Excellence for Innovation in Chemistry (PERCHCIC). The authors thank the Agricultural Research Development Agency (ARDA) (Grant
Number – PRP 5605020800). NS thanks the partial support from the Graduate Studies of
Mahidol University Alumni Association.
Keywords: colorimetric reaction, image detection, microfluidic
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