ISO9001
Pre-stained
Dokdo-MarkTM Broad-range Protein Markers
Product
Volume
Cat. No.
Broad-range Pre-stained
Marker (all blue)
500 ml
EBM-1031
Broad-range Pre-stained
Marker (dual color)
500 ml
Broad-range Pre-stained
Marker (multi color)
500 ml
EBM-1033
Broad-range Marker
(un-stained)
500 ml
EBM-1034
EBM-1032
Remarks
all blue labeled marker
blue and orange banding
during electrophoresis
DokDo-MARKTM
Broad-range
(all blue)
Cat. No. EBM-1031
DokDo-MARKTM
Broad-range
(dual color)
Cat. No. EBM-1032
kDa
240
140
100
kDa
kDa
250 orange
140 blue
105 violet
70 orange
blue, orange, red, violet,
yellow and green banding
50
50 blue
un-stained marker for
exact and sharp banding
35
35 blue
Chromophores are covalently linked to proteins in pre-stained markers, and 10 pre-stained
proteins are visible during electrophoresis or electrophoretic transfer from the gel to the membrane.
The 25 and 70 kDa proteins play a ruler function for easy identification of banding.
Because coupling of chromophores to the proteins in pre-stained markers affects their
apparent molecular weights in SDS-PAGE relative to un-stained proteins, un-stained
protein standard (Cat. No. EBM-1034) should be used for accuracy >95%.
Recommended loading volume
- 5 ml/lane for a mini-gel (10x8 cm2, 0.75 mm or 1 mm thick)
- 10-15 ml/lane for a large-gel (30x20 cm2, 1 mm or 1.5 mm thick)
- 2-5 ml/lane for a western transfer onto PVDF or NC membranes
DokDo-MARKTM
Broad-range
(un-stained)
Cat. No. EBM-1034
kDa
240
140
100
70 orange
70
50 yellow
50
25
20
Broad-range Protein Markers are useful for SDS-PAGE analysis of your proteins. The each
marker is a mixture of 10 recombinant purified proteins whose molecular weights are well-defined.
The markers are supplied in a ready-to-use formula (no need to boil before use), and is suitable for
sizing proteins between 7,000 and 240,000 Da by SDS-PAGE.
DokDo-MARKTM
Broad-range
(multi color)
Cat. No. EBM-1033
240 blue
140 blue
100 blue
70
Description
Un-stained
35 green
26 orange
26 orange
20 blue
20 blue
35
25
20
15
15 blue
15 red
15
7 blue
7 blue
7
7
Before & After R250 staining
DokDo-MARKTM
Broad-range
Un-stained
Cat. No. EBM-1034
5.4
DokDo-MARKTM
Broad-range
(Dual color)
Cat. No. EBM-1032
5.2
5
Log (MW)
For research use only
4.8
4.6
4.4
y = -1.2864x + 5.3566
4.2
2
R = 0.9671
4
0
0.2
0.4
Recommended storage condition
- Store at -20oC for one year
- Please aliquot in a small volume for prevention of repeated freezing & thawing
- Repeated freezing & thawing, long-term storage and re-use of pipette tip may degrade proteins
rapidly.
- The marker is provided as a ready to load format, meaning that there is no need to boil before
use. But precipitation of SDS may occur by long-term storage at below 4oC, and you can load
marker just after dissolving the precipitant by raising to 50-80oC for a few minutes.
- The slight size difference can be seen in the course of manufacturing. Please confirm the batch
numbers.
5.4
5.2
Log (MW)
Cautions
0.6
Rf
0.8
1
DokDo-MARKTM
Broad-range
(Dual color)
Cat. No. EBM-1032
5
4.8
4.6
4.4
4.2
y = -1.3208x + 5.3855
2
R = 0.9668
4
0
0.2
0.4
0.6
0.8
1
Rf
㈜ 엘피스바이오텍
www.elpisbio.com
[302-854] 123-12 Chunglim-Dong, Seo-Gu, Taejeon, Korea
Tel: +82-42-581-8448. Fax: +82-42-581-8449
Standard curves are generated by plotting log molecular weight (MW) versus
migration distance (Rf) of each band in Dokdo-MARK through an GradiGelTM II gradient gel.