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AP Biology Pre-lab for Lab 5: Cell Respiration
1.
Write the balanced equation for the complete oxidation of glucose. Is this reaction endergonic or exergonic?
Include the value of ∆G, in kcal/mol
a.
How many moles of oxygen gas are required to oxidize 1.00 mole of glucose?
b.
How many moles of carbon dioxide are produced per mole of glucose?
I. What does this imply about the volume changes that you would observe if you simply sealed the pea
seeds in a flask?
2.
Explain the purpose of the KOH in the experiment. Write the equation for the reaction it will participate in
during this lab.
3.
What, specifically, is the purpose of the respirometers containing only glass beads? Please be specific in your
answer (e.g. “they are a control” is not specific enough)
4.
When you compare the predicted oxygen consumption of dry vs. germinating seeds, what results do you
expect you will get? Explain why you have this expectation
5.
Room temperature is approximately 21ºC. When comparing the predicted oxygen consumption in the room
temperature water bath with that of the cold-water bath, what results do you expect you will get. Explain
why you have this expectation.
6.
After completing the calculations and graphing of the data in the next section, calculate the rate of oxygen
consumption for the following, include appropriate units:
a.
Room Temperature geminating peas
b.
10ºC germinating peas
c.
Room Temperature dry peas
d.
10ºC dry peas
Show the calculation for the room temperature germinating peas below; include the equation, substitution
with units and the answer.
Here is a data table with sample data. Use the information in the lab to complete the table. When you have
completed the data table, graph the data on the provided grid.