Bozeman-Respiration-Walkthrough

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Name ____________________
Bozeman Cellular Respiration Walkthrough
Directions: Watch Paul Anderson discuss how to set up the cellular respiration lab
on the following website:
http://www.youtube.com/watch?v=oL7C9_3biZQ&feature=youtu.be
and answer the following questions.
1. How can you measure the volume of the germinating peas used in the lab?
2. Why do you measure the volume of the germinating peas first, before using
glass beads or non-germinating peas?
3. Why are glass beads added to the non-germinating peas used together?
4. What is the order in which a respirometer is constructed?
5. Why is KOH (potassium hydroxide) used in the respirometer.
6. Why is non-absorbent cotton used in-between the absorbent cotton and the
peas?
7. Why are glass beads used in one of the respirometer?
8. Why is it necessary to leave the respirometer tips of the pipettes out of the
water for about 10 minutes?
9. What time intervals will you need to take readings?
10. What should happen to the respirometer’s pipette measurements as time
goes by? Explain your answer.
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