Table s6 : Identity of 71 hepatic mRNAs whose translation is altered under proinflammatory
cytokine challenge.
The mRNAs with an up- or down-regulated translation after 30 min or 16 hours of CM challenge are
listed as 4 sets and they are ranked from the most induced to the most repressed translation. These
mRNAs are identified by an IMAGE clone number as a unique identifier.
Encoded protein
IMAGE
Induction or
Change in
repression overall mRNA
of translation abundance b
(fold) a
30 min. CM / up-regulated polysome fraction (17 mRNAs)
polymerase (RNA) II (DNA directed) polypeptide F
RAB3A interacting protein (rabin3)
complement component 3a receptor 1
ubiquitin protein ligase E3A
forkhead box A2 (HNF3, beta)
EST
histone deacetylase 1
lipin 2
cancer susceptibility candidate 3
leukocyte receptor cluster (LRC) member 4
Transcribed sequences
proteasome subunit, beta type, 6
calcium/calmodulin-dependent serine protein kinase
cofilin 2 (muscle)
myosin phosphatase-Rho interacting protein
PAI-1 mRNA-binding protein
Transcribed sequence
767817
824138
home PCR
293557
2204312
109209
754345
111965
40515
811033
292334
712047
201075
78272
108427
82412
84400
4.41 *
3.98
3.73
3.69
3.22
3.12
3.05
2.99
2.81 *
2.79
2.78
2.58
2.50
2.47
2.40
2.37
2.32
+ (C9)
30 min. CM / down-regulated polysome fraction (17 mRNAs)
splicing factor, arginine/serine-rich 2
nucleoporin 205kDa
inhibitor of DNA binding 4
KIAA1522 protein
anaphase-promoting complex subunit 7
calmodulin 1 (phosphorylase kinase, delta)
nucleoporin like 2
hypothetical protein DKFZp313N0621
membrane cofactor protein
sirtuin 5
thioredoxin (Trx)-related protein, 14 kDa
high mobility group nucleosomal binding domain 4
leukotriene B4 12-hydroxydehydrogenase
glyoxalase I
chromosome 20 open reading frame 110
Gardner-Rasheed feline sarcoma viral oncogene homolog
RAB18, member RAS oncogene family
295067
197515
146879
140698
344644
341245
30993
143851
194455
245549
346970
347703
345055
343498
366167
347751
25982
3.91
3.98
4.05
4.15
4.31
4.34
4.36
4.48 *
4.56
4.72
4.74
5.08
5.17
6.14
6.32
6.34
20.55
+ (C11)
16 hours CM / up-regulated polysome fraction (27 mRNAs)
cDNA FLJ40637 fis, clone THYMU2015984
tensin-like SH2 domain-containing 1
pyruvate carboxylase
ribosomal protein L41
H2A histone family, member Y
solute carrier family 43, member 3
TcD37 homolog
EST
zinc finger protein 251
metallothionein 1H
3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2
polymerase (RNA) II (DNA directed) polypeptide F
guanine nucleotide binding protein, alpha 11 (Gq class)
hypothetical protein MGC14327
hypothetical protein FLJ12903
chromosome 8 open reading frame 4
dTDP-D-glucose 4,6-dehydratase
signal transducing adaptor molecule 2
lymphotoxin beta receptor (TNFR superfamily, member 3)
leukemia inhibitory factor (cholinergic differentiation factor)
SEC24 (S. cerevisiae) related gene family, member B
mitogen-activated protein kinase kinase 1
cancer susceptibility candidate 3
suppressor of Ty (S.cerevisiae) 4 homolog 1
Transcribed sequences
FLJ10378 protein
ribosomal protein S23
25750
199619
26619
143147
211116
113544
322771
211859
206848
196003
210684
767817
266294
269007
49137
323314
357777
825041
810443
153025
265450
490119
125162
240606
42166
260181
297010
19.04
15.99
2.06
8.64
8.53
5.95
5.90
5.15
4.75
4.63
4.12
3.99 *
3.99
3.96
3.74
3.60
3.55
3.36
3.15
3.05
2.86
2.84
2.84 *
2.84
2.82
2.81
2.75
+ (C9)
+ (C9)
+ (C9)
+ (C11)
+ (C11)
+ (C9)
16 hours CM / down-regulated polysome fraction (13 mRNAs)
monoamine oxidase B
114906
paired basic amino acid cleaving system 4
204666
tyr 3-monooxygenase/trp 5-monooxygenase activ. protein
142350
Human hbc647 mRNA sequence
134270
cytokine-like nuclear factor n-pac
359636
TIA1 cytotoxic granule-associated RNA-binding protein-like1 341896
ubiquinol-cytochrome c reductase, Rieske iron-sulfur polyp.1 248800
DKFZP564M182 protein
345160
development and differentiation enhancing factor 1
139784
TNF receptor superfamily, member 11a, activator of NFB 346544
eukaryotic translation initiation factor 5A
345374
hypothetical protein DKFZp313N0621
143851
centrosome-associated protein 350
346892
a
2.78
2.81
2.86
3.05
3.24
3.26
3.43
3.52
3.91
4.05
4.14
4.52 *
5.96
+ (C10)
a star indicates that a translational regulation was observed after both durations of CM challenge.
+ or – indicates that the overall abundance of this mRNA was also up- (+) or down- (-) regulated by the
CM challenge, in which case the relevant cluster C1 to C11 (according to Figure 1 and Table s2) is
noted.
b