The Arristaless-like (Arl) gene below carries a homeodomain DNA binding domain (HD).
Quiz 10: Biol 203 Fall 2013
Furthermore, it has a proven activator domain (A) and repressor domain (R).
Name:_______________
Possible WT mRNAs with A-HD, which have activator-like properties as a protein, promote cell growth
Instructor Name (2pt):
Possible WT mRNAs with R-HD, which have repressor-like properties as a protein, inhibit cell growth.
_______________
mRNAs with HD alone never occur. WT mRNAs with A-R-HD code for non-functional proteins.
Arl expression is only observed when all three TxFactors (E1, E2, and E3) are present.
22pts
pA site
Arl
E1
A
E2
R
SD SA
Rag1 is expressed in cells expressing only
E1 and E2.
Rag2 is expressed in cells expressing E1 and E2
as well as in cells expressing all 3 TxFactors
Q1(10pts).
In what cells will activator like properties of
Arl be observed?
HD
SD SA
E3
means enhancer binding site
RSS sites
In which cells will Exon 1 and Exon 2 be
joined within the mRNA?
Express TxFactors E1
Express TxFactor E1, E2
Express TxFactors E1, E2, E3
30 cells in
WT Antennal
Tissue
Let’s assume there is Rag mediated recombination in the brain.
Design a construct that you would use to make a transgenic animal.
This construct would allow you to see GFP fluorescence in any cell only after DNA recombination.
Q2(10pts).
Draw out and describe a PCR screen to complement the transgenic experiment. You may label the transgene
above with primer sites. Make sure that you provide gel electrophoresis results with and without transgenic
vector in the animal.