Seminar of School of Bioresources and Technology
Biotechnology Program
BIT692 (Seminar II)
Date
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Speaker
Title
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04 November 2015
Wednesday, 14.00, EXC Building
Mr. Wudtichai Manasatienkij (Hong)
Viral Discovery by Next-Generation Sequencing
Abstract
Viruses are the cause of death and disease worldwide, especially, the pathogenic RNA viruses.
The viral RNA genomes have high mutation rate that always lead to the emergence of the novel
viruses. As for influenza virus, there are more than 250,000 deaths every year around the world
with the virus and frequently emerge a novel virulent strain [1]. If the viral diagnostic and
develop vaccines too late. The novel virus’s outbreak could become pandemic [2]. In this
situation, rapid discovery and identification of the emergence and re-emergence of viral
diseases are extremely important to control the spread of viral agents and prevention. There are
many techniques are able to identify and discover the novel viruses [4]. However, these methods
have limitations such as time-consuming, costly and prior knowledge of similar viruses [3, 4].
The recent emergence of Next-Generation Sequencing (NGS) techniques are tools, that
provide high-throughput sequence data and possible to sequence all viral genomes in only one
reaction without comparisons with known viruses [4]. NGS techniques are able to sequence a
mixture of genetic materials from a very heterogeneous mix with high sensitivity, low cost and
fast turnaround time [6]. As the results of demonstrate the utility of NGS for pathogen discovery
and identification of 2009 pandemic H1N1 influenza A virus [7], investigate the cause of death in
the United States [8] and discovery mutations in viral quasispeices of A/California/07/2009
(H1N1) vaccine strains passaged in eggs [9]. NGS are able to identify the virus without prior
knowledge of sequence with high sensitivity and suitable for monitoring of viral mutation [7, 8,
9].
Keywords: mutation, next-generation sequencing, virus, viral discovery
REFERENCES
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