Amyloid β on Nobel Metals: surface-enhanced
Raman spectroscopy investigates the structure,
while AFM investigates the function
Debanjan Bhowmik1, Christina MacLaughlin2, Shell Ip2, Suman Nag1, Bidyut
Sarkar1, C. Muralidharan1, Gilbert Walker2 and Sudipta Maiti1*
1
Department of Chemistry, TIFR, Mumbai
Department of Chemistry, Lash Miller Laboratories, UT, Toronto
*maiti@tifr.res.in
2
Medicinal activity of a molecule is highly dependent on the presence of a
biologically important unit. We have designed two keto-tetrahydrocarbazole
derivatives 2-((9-methyl-1-oxo-2,3,4,9-tetrahydro-1H-carbazol-6-yl)oxy)acetic
acid and 2-((7-methoxy-9-methyl-1-oxo-2,3,4,9-tetrahydro-1H-carbazol-6yl)oxy)acetic acid, both of which are efficient fluorophores. We have used
rigorous spectroscopic investigations to elucidate the photophysical
properties of the compounds in different solvents. Absorption maxima of the
compounds have changed to a small extent in solvents with largely different
empirical polarity parameters [ET(30)]. The fluorescence emission maxima
of the compounds, on the contrary, have undergone red shift with increasing
[ET(30)] of the solvents. Protic solvents were especially influencial in
perturbing the excited states of the fluorophores. Calculations of dipole
moment, free energy of solvation, reorganization energy, solvatochromic
comparison method have been used to describe the excited state properties
of the compounds. Binary solvent mixtures were used to demonstrate the
dielectric enrichment of protic solvents in comparison with aprotic solvents.
Since their discovery, flavins have been recognized to play pivotal role in
versatile biochemical redox reactions as they functions as both electrofiles
and nucleophiles inside the complex biological enviroment. In this
presentation we decipher the reaction pathways of Riboflavin (Vitamin B2) in
presence of organic bases (N, N- Dimethylaniline and Triethylamine) in both
homogeneous and heterogeneous media.
References:
[1] Ellis, R. J. Trends Biochem. Sci. 2001, 26, 597 – 604.
[2] Minton, A. P. J. Biol. Chem. 2001, 276, 10577-10580.
[3] Hall, Damien.; Minton, Allen P. Biochimica et Biophysica Acta.2003, 1649, 127139.
[4] Schlesinger, A.P.; Wang, Y.; Tadeo, X.; Millet, O.; Pielak, G.J. J. Am. Chem. Soc.
2011, 133, 8082 – 8085.
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