Table S1. Analysis of plate readings
A. Protein-plus test plates
Wavelength,
nm
T=∑(Abs well A1 to P1)
U=∑(Abs well A2 to P2)
(T + U)/32
350
360
370
etc…
500
∑(A1350+ B1350+…P1350)
∑(A1360+ B1360+…P1360)
∑(A1370+ B1370+…P1370)
etc…
∑(A1500+ B1500+…P1500)
∑(A2350+ B2350+…P2350)
∑(A2360+ B2360+…P2360)
∑(A2370+ B2370+…P2370)
etc…
∑(A2500+ B2500+…P2500)
X350
X360
X370
etc…
X500
Corrected
absorbance
in well A3
A3350-X350
A3360-X360
A3370-X370
etc…
A3500-X500
Corrected
absorbance
in well A4
A4350-X350
A4360-X360
A4370-X370
etc…
A4500-X500
Corrected
absorbance
in well A5
A5350-X350
A5360-X360
A5370-X370
etc…
A5500-X500
Corrected
absorbance
in well A3
A3350-Y350
A3360-Y360
A3370-Y370
etc…
A3500-Y500
Corrected
absorbance
in well A4
A4350-Y350
A4360-Y360
A4370-Y370
etc…
A4500-Y500
Corrected
absorbance
in well A5
A5350-Y350
A5360-Y360
A5370-Y370
etc…
A5500-Y500
etc…
etc…
etc…
etc…
etc…
etc…
Corrected
absorbance
in well A24
A24350-X350
A24360-X360
A24370-X370
etc…
A24500-X500
B. Protein-minus reference plates
Wavelength,
nm
V=∑(Abs well A1 to P1)
W=∑(Abs well A2 to P2)
(V + W)/32
350
360
370
etc…
500
∑(A1350+ B1350+…P1350)
∑(A1360+ B1360+…P1360)
∑(A1370+ B1370+…P1370)
etc…
∑(A1500+ B1500+…P1500)
∑(A2350+ B2350+…P2350)
∑(A2360+ B2360+…P2360)
∑(A2370+ B2370+…P2370)
etc…
∑(A2500+ B2500+…P2500)
Y350
Y360
Y370
etc…
Y500
etc…
etc…
etc…
etc…
etc…
etc…
Corrected
absorbance
in well A24
A24350-Y350
A24360-Y360
A24370-Y370
etc…
A24500-Y500
Where A through P are absorbance values in corresponding wells in 384-well screening plates (Fig. 1) at specified wavelengths; T is the sum of
absorbance values from A1 to P1 and U is the sum of absorbance values from A2 through P2 in plate A at specified wavelengths; V is the sum of
absorbance values from A1 to P1 and W is the sum of absorbance values from A2 through P2 in plate B at specified wavelengths; X is an
absorbance of the buffer, DMSO and protein background averaged across the columns 1 and 2 in plate A for each individual wavelength, Y is an
absorbance of the buffer and DMSO background averaged across the columns 1 and 2 in plate B for each individual wavelength