2023-11-04T19:55:54+03:00[Europe/Moscow] en true <p>Bacterial growth requirements</p>, <p>How to maintain desired temperature of medium?</p>, <p>How to adjust the pH of the medium?</p>, <p>oxygen classes of microorganisms</p>, <p>obligate aerobe</p>, <p> anaerobe</p>, <p>facultative anaerobe</p>, <p>microaerophiles</p>, <p>aerotolerant anaerobe</p>, <p>temperature classes of microorganisms</p>, <p>pasteurisation</p>, <p>where do you grow anaerobes?</p>, <p>obligate anaerobe</p>, <p>osmotic pressure</p>, <p>bacterial growth factors</p>, <p>medium (plural: media)</p>, <p>media state of matter</p>, <p>what can be used for solidification of media?</p>, <p>colony</p>, <p>types of growth media</p>, <p>special growth media types</p>, <p>selective media</p>, <p>enrichment media</p>, <p>differential media</p>, <p>How many cells will I have after certain number of generations?</p>, <p>How many generations did I haven this time?</p>, <p>How long does it take for cells to double?</p>, <p>How long does my culture grow?</p>, <p>How many cells will I have after a certain time?</p>, <p>How fast does my culture grow?</p>, <p>Phases of growth</p>, <p>batch culture</p>, <p>batch culture types</p>, <p>Fed-batch</p>, <p>continuous culture </p>, <p>chemostat</p>, <p>autoclave</p>, <p>bactericidal agent</p>, <p>bacteriostatic agent</p>, <p>biofilm</p>, <p>exponential growth</p>, <p>generation time</p>, <p>growth</p>, <p>MIC</p>, <p>plate count</p> flashcards
Microbiology 2.

Microbiology 2.

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  • Bacterial growth requirements

    -temperature

    -pH

    -oxygen

    -osmotic pressure

    -hydrostatic pressure

    -light

    -nutrients

  • How to maintain desired temperature of medium?

    incubator/shaking incubator

  • How to adjust the pH of the medium?

    buffers

  • oxygen classes of microorganisms

    -obligate aerobe-obligate anaerobe-facultative anaerobe-microaerophiles-aerotolerant anaerobe

    -obligate aerobe

    -obligate anaerobe

    -facultative anaerobe

    -microaerophiles

    -aerotolerant anaerobe

  • obligate aerobe

    an organism that can use O2 in respiration, require O2 some

  • anaerobe

    an organism that cannot use O2 in respiration and whose growth is typically inhibited by O2

  • facultative anaerobe

    with respect to O2, an organism that can grow in either its presence or absence

  • microaerophiles

    an aerobic organism that can grow only when O2 tensions are reduced from that present in air

  • aerotolerant anaerobe

    a microorganism unable to respire O2, but whose growth is unaffected by it

  • temperature classes of microorganisms

  • pasteurisation

    the heat treatment of milk or other liquids to reduce its total number of microorganisms

  • where do you grow anaerobes?

    CO2 incubators

  • obligate anaerobe

    an organisms that cannot grow in the presence of O2

  • osmotic pressure

    the minimum pressure applied to a solution to stop the flow of solvent molecules through a semipermeable membrane

  • bacterial growth factors

    -vitamins

    -amino acids

    -purines and pyrimidines and their precursors

  • medium (plural: media)

    any solid or liquid specially prepared for bacterial growth

  • media state of matter

    liquid:

    -broth

    solid:

    -plates

    -tubes

    -slants

  • what can be used for solidification of media?

    -Agar-Gelatine

    -Agar

    -Gelatine

  • colony

    millions of bacteria formed from one bacterium

  • types of growth media

  • special growth media types

    - selective media

    - enrichment media

    - differential media

  • selective media

    suppression of unwanted bacteria and encouraging desired microbes

  • enrichment media

    similar to selective media but designed to increase numbers of desired bacteria to detectable levels

  • differential media

    allows the differentiation of desired microbes from others eg:by color reaction

  • How many cells will I have after certain number of generations?

  • How many generations did I haven this time?

  • How long does it take for cells to double?

  • How long does my culture grow?

  • How many cells will I have after a certain time?

  • How fast does my culture grow?

  • Phases of growth

    -lag phase: little or no cell division, cells are accommodating to the new environment

    -log phase: exponential growth

    -stationary phase: a nutrient runs outer waste products accumulate

    -death phase: exponential death rate

  • batch culture

    a closed-system microbial culture of fixed volume

  • batch culture types

    -fed-batch culture

    -continuous culture

  • Fed-batch

  • continuous culture

  • chemostat

    a device that allows for the continuous culture of microorganisms with independent control of both growth rate and cell number

  • autoclave

    a sealed heating device that destroys microorganisms with temperature and steam under pressure

  • bactericidal agent

    an agent that kills bacteria

  • bacteriostatic agent

    an agent that inhibits bacterial growth

  • biofilm

    an attached polysaccharide matrix containing bacterial cells

  • exponential growth

    growth of a microbial population in which cell numbers double within a specific time

  • generation time

    the time required for a population of microbial cells to double

  • growth

    an increase in cell numbers

  • MIC

    minimum inhibitory concentration: the minimum concentration of a substance necessary to prevent microbial growth

  • plate count

    a method of counting viable cells, the number of colonies on a plate is used as a measure of cell numbers