2024-10-03T01:18:43+03:00[Europe/Moscow] en true <p>Culture media </p>, <p>Sterilization </p>, <p>Steam pressure by pressure cooker or autoclave</p>, <p>Hot air sterilizer or oven </p>, <p>15 psi (121) for 15-20 mins </p>, <p>150-170 </p>, <p>Solid culture media </p>, <p>100</p>, <p>40-45</p>, <p>Liquid culture media </p>, <p>Eosine methylene blue agar</p>, <p>Greenish metallic sheen dark center </p>, <p>10 drops Bromcresol green with 0.01 HCL </p>, <p>10 drops of Bromthymol blue with 0.01 HCL </p>, <p>10 drops of phenolphthalein indicator with 0.01 N NaOH</p>, <p>Yellow</p>, <p>Green </p>, <p>Fuchsia pink</p> flashcards
Microbio lab 4

Microbio lab 4

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  • Culture media

    is any nutrient material prepared for the growth and cultivation of microorganisms

  • Sterilization

    is a process whereby any material is entirely freed from living organism

  • Steam pressure by pressure cooker or autoclave

    sterilization of culture media

  • Hot air sterilizer or oven

    sterilization of glasswares

  • 15 psi (121) for 15-20 mins

    saturated steam/ moist medium

  • 150-170

    dry air

  • Solid culture media

    with agar solidifying agent

  • 100

    melting point of Solid CM

  • 40-45

    solidifying point of Solid CM

  • Liquid culture media

    usually in broth form mixed growth of microorganism

  • Eosine methylene blue agar

    Differential culture media

  • Greenish metallic sheen dark center

    EMBA

  • 10 drops Bromcresol green with 0.01 HCL

    pH 4.0

  • 10 drops of Bromthymol blue with 0.01 HCL

    pH 6-7.6

  • 10 drops of phenolphthalein indicator with 0.01 N NaOH

    pH 8.3-10.0

  • Yellow

    ph 4 color

  • Green

    pH 7

  • Fuchsia pink

    pH 8