Culture media
is any nutrient material prepared for the growth and cultivation of microorganisms
Sterilization
is a process whereby any material is entirely freed from living organism
Steam pressure by pressure cooker or autoclave
sterilization of culture media
Hot air sterilizer or oven
sterilization of glasswares
15 psi (121) for 15-20 mins
saturated steam/ moist medium
150-170
dry air
Solid culture media
with agar solidifying agent
100
melting point of Solid CM
40-45
solidifying point of Solid CM
Liquid culture media
usually in broth form mixed growth of microorganism
Eosine methylene blue agar
Differential culture media
Greenish metallic sheen dark center
EMBA
10 drops Bromcresol green with 0.01 HCL
pH 4.0
10 drops of Bromthymol blue with 0.01 HCL
pH 6-7.6
10 drops of phenolphthalein indicator with 0.01 N NaOH
pH 8.3-10.0
Yellow
ph 4 color
Green
pH 7
Fuchsia pink
pH 8