2022-09-19T23:39:41+03:00[Europe/Moscow] en true <p>lack; 3'-hydroxy group</p>, <p>termination of chain elongation</p>, <p>restriction endonucleases</p>, <p>sense, coding</p>, <p>anti-sense, non-coding</p>, <p>promoters</p>, <p>introns</p>, <p>Exons</p>, <p>Untranslated Regions</p>, <p>TATA</p>, <p>- rna polymerase binds to dsDNA at promoter region</p><p>-promoter region consists of TATA sequences</p><p>-genes are in inactive state &amp; require transcription factors (activators)</p>, <p>3'-5'</p>, <p>alternate splicing</p>, <p>LEQVIO (Inclisiran)</p>, <p>PCSK9</p>, <p>Latent HIV reservoir</p>, <p>Reverse transcriptase</p>, <p>HIV integrase</p>, <p>Components of HIV integrase:</p>, <p>NRTIs (Nucleoside/Nucleotide RT inhibitors) </p>, <p>prodrugs, not</p>, <p>-tegravir</p>, <p>HIV INSTI</p>, <p>integrase inhibitor, NRTI</p> flashcards
Genomics slide deck 3

Genomics slide deck 3

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  • lack; 3'-hydroxy group

    Dideoxynucleotides _____ a ________ on sugar

  • termination of chain elongation

    Addition of a dideoxynucleotide (ddn) to the 3'-OH end of a DNA chain results in what?

  • restriction endonucleases

    cut dsDNA at specific sequences

  • sense, coding

    DNA non-template strand =

  • anti-sense, non-coding

    DNA template strand =

  • promoters

    region of DNA upstream of initiation of transcription

  • introns

    extra sequences between polypeptide-specifying portions, are not expressed, interrupt most eukaryotic genes

  • Exons

    portions of a gene that are expressed

  • Untranslated Regions

    regulate mRNA stability, transport, and translation efficiency

  • TATA

    Promoter regions are denoted by what nomenclature?

  • - rna polymerase binds to dsDNA at promoter region

    -promoter region consists of TATA sequences

    -genes are in inactive state & require transcription factors (activators)

    Explain RNA synthesis.

  • 3'-5'

    During elongation, RNA polymerase "walks" along one strand of DNA, known as the template strand, in the _______ direction.

  • alternate splicing

    where more than one mRNA can be made from the same gene. This results in the production of more than one protein from translation.

  • LEQVIO (Inclisiran)

    -siRNA medication that has the potential to be used in the wide prevention of a common disease, atherosclerosis.

    -delivered to hepatocytes

    -RISC & AS strand bind to PCSK9 mRNA

  • PCSK9

    gene responsible for producing PCSK9 protein; this protein promotes LDL-C production.

  • Latent HIV reservoir

    -the presence of transcriptionally silent but replication-competent proviral genome.

    -allows infected cells to evade both immune clearance mechanisms.

  • Reverse transcriptase

    responsible for the transcription of the viral single-stranded RNA genome to proviral double stranded DNA.

  • HIV integrase

    integrates proviral double stranded DNA into host genome.

  • Components of HIV integrase:

    -288 amino acids

    -catalytic core contains 3 amino acids that coordinate binding with a divalent metal cofactor (either Mg2+ or Mn2+), forming the active catalytic site.

  • NRTIs (Nucleoside/Nucleotide RT inhibitors)

    -utilizes 3 phosphorylation steps catalyzed by cellular kinases

    -convert the 2',3'- dideoxynucleoside to its 5'- triphosphate nucleotide derivative

    -acts as chain terminator

  • prodrugs, not

    NRTIs are _____ while NNRTIs are _____

  • -tegravir

    What is the suffix for HIV- integrase strand transfer inhibitors?

  • HIV INSTI

    -HIV integrase strand-transfer inhibitors (INSTIs) stop HIV from replicating by blocking the viral integrase and are widely used in HIV treatment.

  • integrase inhibitor, NRTI

    The Panel on Antiretroviral Guidelines for Adults and Adolescents Recommends 5 Initial Regimens for Most People with HIV that are ART-naive: they all include an ____ & ______