2021-12-21T16:37:11+03:00[Europe/Moscow] en true <p>size width estimate of a slide</p>, <p>why do samples need to be thin</p>, <p>solid specimens (dry mount) slide preparation</p>, <p>examples of solid specimens (dry mount)</p>, <p>wet specimens (wet mount) slide preparation</p>, <p>examples of wet specimens (wet mount) </p>, <p>soft specimens (squash slides) slide preparation</p>, <p>examples of soft specimens (squash specimens)</p>, <p>body fluid specimens ( smear slides) slide preparation</p>, <p>examples of body fluid specimens (smear slides) </p>, <p>why slides need to be stained</p>, <p>process to stain a specimen</p>, <p>used to heat a slide</p>, <p>type of stain dependent on </p>, <p>stains cell walls purple, used in gram staining</p>, <p>stains the nuclei in animal cells to give contrast</p>, <p>negative stain that is not taken up by the cell but provides a contrast between the cell and the background</p> flashcards

AS level CIE Microscope Slide Preparation

revision notes on syllabus section 1.1

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  • size width estimate of a slide

    thin

  • why do samples need to be thin

    to allow enough light to pass through

  • solid specimens (dry mount) slide preparation

    thin slices called sectioning, coverslip placed on top

  • examples of solid specimens (dry mount)

    hair, pollen, dust, muscle tissue, plant tissue

  • wet specimens (wet mount) slide preparation

    suspended in water or immersion oil, coverslip placed at an angle

  • examples of wet specimens (wet mount)

    aquatic samples and other living organisms

  • soft specimens (squash slides) slide preparation

    wet , mount squashed between slide and coverslip

  • examples of soft specimens (squash specimens)

    root cells to look at cell division

  • body fluid specimens ( smear slides) slide preparation

    the edge of the slide is used to smear the sample , creating thin even coating .

  • examples of body fluid specimens (smear slides)

    blood smears to view erythrocytes

  • why slides need to be stained

    cell structures may be transparent or difficult to distinguish

  • process to stain a specimen

    air dried and heated

  • used to heat a slide

    bunsen burner flame

  • type of stain dependent on

    type of specimen used

  • stains cell walls purple, used in gram staining

    crystal violet stain

  • stains the nuclei in animal cells to give contrast

    methylene blue stain

  • negative stain that is not taken up by the cell but provides a contrast between the cell and the background

    congo red stain