size width estimate of a slide
thin
why do samples need to be thin
to allow enough light to pass through
solid specimens (dry mount) slide preparation
thin slices called sectioning, coverslip placed on top
examples of solid specimens (dry mount)
hair, pollen, dust, muscle tissue, plant tissue
wet specimens (wet mount) slide preparation
suspended in water or immersion oil, coverslip placed at an angle
examples of wet specimens (wet mount)
aquatic samples and other living organisms
soft specimens (squash slides) slide preparation
wet , mount squashed between slide and coverslip
examples of soft specimens (squash specimens)
root cells to look at cell division
body fluid specimens ( smear slides) slide preparation
the edge of the slide is used to smear the sample , creating thin even coating .
examples of body fluid specimens (smear slides)
blood smears to view erythrocytes
why slides need to be stained
cell structures may be transparent or difficult to distinguish
process to stain a specimen
air dried and heated
used to heat a slide
bunsen burner flame
type of stain dependent on
type of specimen used
stains cell walls purple, used in gram staining
crystal violet stain
stains the nuclei in animal cells to give contrast
methylene blue stain
negative stain that is not taken up by the cell but provides a contrast between the cell and the background
congo red stain