Vmax (s)/ km + (s)
What is the equation for Michaelis-Menten?
reaction rate will increase
What happens if you increase the concentration of the substrate?
Vmax
represents the maximum rate achieved by the system
Km
is the substrate concentration at which the reaction rate if half of Vmax
double reciprocal; lineweaver-burk
Michaelis-Menton equation can be rearranged to _________ plot and this will give us a linear equation called ____________
increasing substrate concentration; decreasing product concentration
Inhibiting an enzyme blocks the degradation of its substrate by ___________ and blocks the formation of its product ____________
chemotherapy
-the use of drugs to combat foreign organisms and aberrant cells
by targeting enzyme that is vital for bacteria, but not humans
How do antibiotics work?
Ideal inhibitor
-totally specific for one target enzyme; rarely occurs
selective toxicity; due to higher metabolic needs
Chemotherapy kills tumor cells by virtue of _______
Irreversible
Inhibition of an enzyme that involves a covalent bond.
reversible
inhibition of enzyme activity that is typically noncovalent
competing with substrate at active site
The most common method reversible enzyme inhibitors use to inhibit enzymes is ____________
b
Smaller Ki tells us that the inhibitor is
a) ineffective at binding
b) more effective at binding
c) utilizing a covalent bond
d) irreversible
it will decrease & lead to less product
If I increase the concentration of inhibitor, what will happen to the [ES] complex?
IC50
-the inhibitor concentration that produces 50% enzyme inhibition or produce a 50% decrease in enzyme activity
more potent
Smaller IC50 value tells us what?
competitive
In _________ inhibition, the inhibitor competes with the substrate for the same binding site
c
In competitive inhibiton, the inhibitor binds to :
a) ES complex
b) Substrate only
c) Free enzyme only
d) all of the above
apparent Km; Vmax
Competitive inhibitors alter the ________ not the _________
c
In noncompetitive inhibition, the inhibitor binds to:
a) free enzyme only
b) ES substrate only
c) Free enzyme & the ES complex
d) none of the above
vmax; km
Noncompetitive inhibitor alters the ______ not the ________
A
In uncompetitive inhibition, the inhibitor binds to:
a) ES complex only
b) Free enzyme
c) Free enzyme & the ES complex
d) substrate only
B
Uncompetitive inhibitors decrease:
a) V max only
b) Vmax & Km
c) Km only
d) keeps them the same
noncompetitive
This graph represents:
uncompetitive
This graph represents:
competitive
This graph represents