Osteon/Haversian system (comprises most of compact bone)
Bone Preparation: Decalcified Calcium
Calcium salts are removed through treatment of the tissue
Then bone is cut and stained normally
Trichromeis usually used-bone will stain blue, other tissues pink to red.
Ground
Bone is sawed into thin sections and then ground down until it can be observed using light microscopy.
No Staining.