ERASMUS Intensive Program SYNAPS: Synthesis and Retrosynthesis in the Chemistry of Natural Products July 2011 NATURAL PRODUCT CHEMISTRY Module 2 Retrosynthetic Approaches Towards the Synthesis of Natural Products DEPARTMENT OF CHEMISTRY UNIVERSITY OF CRETE Lifelong Learning Program – Erasmus Hellenic National Agency LLP-Ι.Κ.Υ. INTRODUCTION Based on: S. Warren Organic Synthesis: The Disconnection Approach, Wiley: New York, 1982 • • • • • • • • • • • Chemists synthesize compounds in just about every organic chemistry laboratory in the world. Industrial chemists synthesize pharmaceuticals, polymers (plastics), pesticides, dye stuffs, food colorings and flavorings, perfumes, detergents and disinfectants. Research chemists synthesize natural products whose structure is uncertain, compounds for mechanistic investigations, possible intermediate in chemical and biological processes, thousands of potential drugs used in medical practice, and even compounds which might themselves be useful for organic syntheses. Before and during these syntheses, groups of chemists sitting around blackboards or piles of paper plan the work they are about to undertake. Possible routes are drawn out, criticized, modified again when the behavior of the compounds in the flask turns out to be different from what was expected, until finally success is achieved. The aim of this lecture is to show how this planning is done: to help you learn the disconnection or synthon approach to organic synthesis. This approach is analytical: we start with the molecule we want to make (the target molecule) and break it down by a series of disconnection into possible starting materials. Classifications in Synthetic Methodology Based on “Lecture Notes, Modern Organic Synthesis” by Dale L. Boger at The Scripps Research Institute, TSRI Press, La Jolla, CA, 1999 Classifications in Synthetic Methodology Classifications in Synthetic Methodology Retrosynthesis or Retrosynthetic Analysis ROUTINE FOR DESIGNING A SYNTHESIS • ANALYSIS 1. 2. 3. 4. Define the target molecule Recognize the functional groups in the target molecule Disconnect using as a guide methods corresponding to known reactions Repeat the Retrosynthetic Analysis till you reach available starting materials • SYNTHESIS 1. Write down a synthetic scheme based in the Retrosynthetic Analysis adding reagents and reaction conditions. 2. If the synthesis fails, modify the synthetic scheme based on the failures/successes in the laboratory experiments. SYNTHONS AND REAGENTS • During the retrosynthetic analysis of compound 2 the retrosynthetic cleavage (or disconnection) leads to a nucleophile(-) and an electrophile (+). The correct alternative, based in known chemical transformations, is in this case leading to 3 and 4. O O a MeO MeO 4 3 b O MeO After the right choice has been made, the synthons may be converted retrosynthetically in to the corresponding reagents. H O MeO MeO O MeO SYNT HONS O Cl REAGENTS ONE GROUP DISCONNECTIONS Based on: S. Warren Organic Synthesis: The Disconnection Approach, Wiley: New York, 1982 1.1 Carbonyl derivatives R(C=O)X a. Carbonyl derivatives R(C=O)X Derived from the corresponding carboxylic acids or their derivatives. O O O O > R > R Cl O O R' > R OR' R NR' 2 Example: Retrosynthesis of Popanil, a weed killer used in the rice fields: O NO 2 NH 2 HN O FGI + C-N Cl Cl Cl Cl Cl Cl Cl Cl Cl C-N Synthesis: NO 2 HNO3 H2SO4 Cl H2, Pd/C Cl Cl NH 2 EtCOCl Cl Cl Cl PROPANIL Cl Cl Cl Cl Cl Cl a. Alcohols, Ethers, Alkyl Halides and Sulfides ROR ROH RSR RX RNu RSH RHal RX= RBr or ROTs or ROMs TWO GROUP DISCONNECTIONS 2.1. 1,1 Difunctionalized Compounds 2.1.1. Acetals OMe OMe O + 2 MeOH OMe 2.1.2. Cyanohydrines CN OH + CN O + HCN OH 2.1.3 Amino Acids: Strecker Synthesis H NH 2 R COOH FGI H NH 2 R CN HCN NH 2 + CN O + NH 3 TWO GROUP DISCONNECTIONS 2.2 2.2.1 1,2-Difunctionalized Compounds Alcohols Cl Ph OH Ph PhMgBr O + Example H N H N C-O O NH 2 OH OH OH O O + O O i. Carbonyl Compounds Nu O O O Nu- + Hal R R R Example: 2,4-D O COOH Cl Cl Cl OH OH COOH Cl Cl Synthesis of 2,4-D OH OH Cl 2, Fe Cl Cl O NaOH Cl COOH Cl Cl COOH TWO GROUP DISCONNECTIONS 2.3 2.3.1 1,3-Difunctionalized Compounds beta-Hydroxycarbonyl Compounds (Aldol Condensation) OH O O + CHO H H 2.3.2 O H a, b-Unsaturated Carbonyl Compounds (Aldol and Dehydration) O2N O 2N O2N + CHO CHO OH Bull. Chem. Soc. Jap. 1952, 25, 54 CHO CH3CHO TWO GROUP DISCONNECTIONS 2.3.3. 1,3-Dicarbonyl Compounds (Claisen-Type Reactions) O O O O O + Example O b Ph Ph Ph + EtO O O O Ph O a a O Ph b OEt Ph O Pathway b is a self-condensation of ethyl phenylacetate. O + O TWO GROUP DISCONNECTIONS 2.4 1,4-Difunctionalized Compounds 2.4.1 2.4.1 1,4-Dicarbonyl Compounds (Enolates and a-Halocarbonyls) O O + R' R R' Br R' R O O O Example O O Br + O O O Practically the synthesis involves the Stork enamine methodology. O N O Br O N N H G. Stork et. al. J. Am. Chem. Soc., 1963, 85, 207 H2O O O TWO GROUP DISCONNECTIONS • g-Hydroxy Carbonyl Compounds (Enolates and Epoxides) 2.4.2 O O O + C 6H13 C6H 13 OH OH TWO GROUP DISCONNECTIONS • • 2.5 1,5-Difunctionalized Compounds 2.5.1. 1,5-Dicarbonyl Compounds (Michael Reaction) O O O O + H H Ph Ph There are two alternative disconnections in this case O O R a b a O O + R R' R' b O O + R' R Sometimes the disconnection is easy to choose. Example O COOEt O O O O COOEt O + + EtO OEt TWO GROUP DISCONNECTIONS • • 2.6 1,6-Difunctionalized Compounds 2.6.1. 1,6-Dicarbonyl Compounds (Ozonolysis of Cyclohexenes) O R R R R O Example OMe O OH OMe O OMe O OMe 3. Regioselectivity 3.1 Regioselective Alkylation of Ketones O R1 O R1 R2 R2 Me Me I ? O R1 R2 Me The use of Activating Groups Analysis O O O X Br Synthesis O O - EtO Na EtO-Na + CO 2Et PrBr CO 2Et Br + 1. OH- O CO 2Et 2. H+ 3. Q O 3. Regioselectivity 3.2 Regioselectivity in Michael Additions R1 R2 Nu R1 R2 O Nu- ? O R1 R2 HO Nu Mechanistic Principles 1. Michael products are the thermodynamic products since the more stable C=O bond is preserved and the weaker C=C bond reacts. 2. Direct addition is more easily reversed than the Michael addition. Therefore, the more stable the Nucleophile the more the Michael addition is favored. 3. Kinetically the C=O carbon is the hard site and the beta carbon is the soft one. Therefore, RLI, NH2-, RO-, H-, attack on the carbonyl and RMgBr, R3N, RS-, and stable carbanions tend to give the Michael type products. 3. Regioselectivity in Michael Reactions Analysis O O O Synthesis O O 1. BuLi Br 2. Cu(I)Cl )2CuLi 4. Chemoselectivity 4.1 The Problems: 1. Relative reactivity of two different Functional Groups NH 2 H N Ac2O HO HO O 2. Reaction of one of two identical Functional Groups OH HO OMe base Me I HO 3. Reaction of a group that may react again. S2- + RBr RS- + RBr RSR 4. Chemoselectivity 4.2 The solution to the problems: Guideline 1: When the two groups are of unequal reactivity, the more reactive can be made to react alone. Paracetamol: Analysis H N HO NH 2 C-N O HO NO2 C-N FGI HO HO 4. Chemoselectivity Guideline 2: When a functional group may react twice, the reaction is successful only when the first product is less reactive that the starting material. Analysis O O Cl O OH Cl Cl Synthesis O OH Cl O Cl O O Cl O O The reaction does not proceed to a second step since the intermediate is stabilized by the resonance effect 4. Chemoselectivity Guideline 3: The above cases could be solved by the use of protective groups. H2N O O R COOH O + O Cl Again, the intermediate is stabilized by the resonance effect NO 2 HNO 3 90% NaHS MeOH H2SO4 NO 2 NH 2 NO 2 NO 2 R N H COOH 4. Chemoselectivity Guideline 4: One of the two identical groups may react if the first product is less reactive than the starting material. NO 2 HNO 3 90% NaHS MeOH H2SO4 NO 2 NH 2 NO 2 NO 2 4. Chemoselectivity Guideline 5: One of the two identical groups may react with one equivalent of reagent using the statistical effect. Na/xylene HO OH HO O- EtBr HO OE t I d on ’t th in k so!!! 4. Chemoselectivity Guideline 6: Use a derivative of the two identical groups which can react only once. O COOH AcOAc Me OH COOMe SOCl 2 COOMe O COOH COOH COCl O Guideline 7: When the two groups are almost but not quite identical, avoid attempts to react only one of them. 4. Chemoselectivity Guideline 7: When the two groups are almost but not quite identical, avoid attempts to react only one of them.