TORTORA  FUNKE  CASE
ninth edition
MICROBIOLOGY
an introduction
1
The Microbial
World and You
Instructor : Dr. Ahmad Saleh
Microbes in Our Lives

Microbiology is the study of microorganisms.

The overall theme of the Microbiology course is to study the relationship
between microbes and our lives.

Microorganisms (microbes) are organisms that are too small to be seen
with the unaided eye, and usually require a microscope to be seen.

This relationship involves harmful effects such as diseases and food
spoilage as well as many beneficial effects.

“Germ” refers to a rapidly growing cell.

Microorganisms include:
1.
Bacteria
2.
Fungi (yeasts and molds)
3.
Microscopic Algae
4.
Protozoa
5.
Viruses, Viroids, Prions
(Non-living infectious agents)
Microbes in Our Lives

These small organisms are usually associated with major diseases such as
AIDS, uncomfortable infections, or food spoilage.

However, the majority of microorganisms make crucial contributions to the
to the welfare of the world’s inhabitants by maintaining balance of living
organisms and chemicals in our environment.

Therefore, Microorganisms are essential for life on earth.

They have important beneficial biological functions:
1. Photosynthesis: Marine and freshwater MO (Algae and some bacteria)
capture energy from sunlight and convert it to food, forming the basis
of the food chain in oceans, lakes, and rivers and generates oxygen
which is critical for life on Earth.
2.
Decomposers: Soil microbes break down dead and decaying matter and
recycle chemical elements that can be used by other organisms.
3. Nitrogen Fixation: Some bacteria can take nitrogen from air and
incorporate it into organic compounds in soil, water, and air.
Microbes in Our Lives
4. Digestion: Human and many other animals have microorganisms in their
digestive tract, that are essential for digestion and vitamin synthesis.
a. Cellulose digestion by ruminants (cows, rabbits, etc.)
b. Synthesis of Vitamin K (for blood clotting) and Vitamin B (for
metabolism) in humans.
5. Synthesis of chemical products: MOs have many commercial applications,
such as the synthesis of acetone, organic acids, enzymes, alcohols.
6. Medicine: Many antibiotics and other drugs are naturally synthesized by
microbes.
 Penicillin is made by a mold.
7. Food industry: many important foods and beverages are made with microbes:
vinegar, pickles, alcoholic beverages, green olives, soy sauce, buttermilk,
cheese, yogurt, and bread.
Microbes in Our Lives
8.
Genetic engineering: recombinant microbes produce important
a.
Medical and therapeutic products: human growth hormone, insuline,
blood clotting factor, recombinant vaccines, monoclonal antibodies,…etc.
b.
9.
Commercial products: cellulose, digestive aids, and drain cleaner.
Medical Research: Microbes are well suited for biological and medical
research for several reasons:
a.
Relatively simple and small structures, easy to study
b.
Genetic material is easily manipulated.
c.
Can grow a large number of cells very quickly and at low cost.
d.
Short generation times make them very useful to study genetic
changes.

Though only a minority of MOs are pathogenic (disease-producing), practical
knowledge of microbes is necessary for medicine and related heath sciences.

Ex.: Hospital workers must be able to protect patients from common
microbes that are normally harmless but pose a threat to the sick and
injured.
Knowledge of Microorganisms

Today we understand that MOs are almost everywhere !

Yet not long ago, before the invention of the microscope,
microbes were unknown to scientists and :

Thousands of people died in devastating epidemics, the
causes of which were NOT understood.

Entire families died because vaccinations and antibiotics
were NOT available to fight infections.

Therefore, knowledge of MOs allows humans to
1. Prevent disease occurrence
2. Prevent food spoilage
3. Led to aseptic techniques to prevent contamination in
medicine and in microbiology laboratories.
Naming and Classifying Microorganisms

Linnaeus established the system of scientific nomenclature (naming)
of organisms in 1735.

Latin was the language traditionally used by scholars.
1.
Scientific nomenclature assigns each organism two names (Binomial):
a.
The genus is the first name and is always capitalized.
b.
The specific epithet (species name) follows and is not
capitalized.
2.
Are italicized or underlined.
3.
The genus is capitalized and the specific epithet is lower case.
4.
Are “Latinized” and used worldwide.
5.
May be descriptive or honor a scientist.
Naming and Classifying Microorganisms
1.
Staphylococcus aureus

Describes the clustered arrangement of the cells (staphylo),
(coccus) indicates spherical shape, and the golden color of the
colonies (aur-).
2.
Escherichia coli

Honors the discoverer, Theodor Escherich, and describes the
bacterium’s habitat–the large intestine or colon.
3.
After the first use, scientific names may be abbreviated with the
first letter of the genus and the specific epithet:

Staphylococcus aureus and Escherichia coli are found in the
human body. S. aureus is on skin and E. coli in the large intestine.
Types of Microorganisms
BACTERIA (Sing. Bacterium)
1. Relatively Simple, single-celled (unicelluar) organisms.
2. Prokaryotic (their genetic material is not enclosed in nuclear membrane)

Prokaryotes include the bacteria and archaea
3. Bacteria appear in one of several shapes:
a. Bacillus (rodlike), b. coccus (spherical),
c. spiral (corkscrew or curved),
d.
some are star-shaped or square.
4. Individual bacteria may form pairs, chains, clusters, or other groupings.
5. Enclosed in cell walls largely composed of peptidoglycan (carbohydrate and
protein complex).
6. Reproduce by binary fission (division into two equal cells)
7. For nutrition, most bacteria use organic chemicals derived from dead or living
organisms.
8. Some bacteria produce their food by photosynthesis, and some can derive
nutrition from inorganic substances.
9. Many bacteria can swim by using flagella (moving appendages).
Types of Microorganisms
ARCHAEA
1.
Consists of prokaryotic cells
2.
If they have cell walls, they lack peptidoglycan
3.
Archaea are not known to cause disease in humans.
4.
Live in extreme environments
5.
Are divided into three main groups:
a.
Methanogens: produce methane as waste product from respiration.
b.
Extreme halophiles: Salt loving, live in extremely salty environments
such as the Great Salt Lake and the Dead Sea.
c.
Extreme thermophiles: Heat loving, live in hot sulfurous water such as
hot springs.
Types of Microorganisms
FUNGI (S. Fungus)
1.
Eukaryotic (have a distinct nucleus containing the cell’s genetic material
surrounded by a nuclear membrane)
2.
Organisms in kingdom Fungi may be Unicellular or multicellular
3.
Multicellular fungi, such as mushroom look like plants, but can not carry out
photosynthesis.
4.
True fungi have cell walls composed of chitin.
5.
The unicellular fungi, yeasts, are oval MOs that are larger than bacteria.
6.
The most typical fungi are molds, composed of visible masses of filaments
(hyphae) called mycelia.
7.
Use organic chemicals for energy, can not carry out photosynthesis.
8.
Fungi can reproduce sexually and asexually
9.
They obtain nutrients by absorbing solutions of organic material from
environment – soil, sea water, fresh water, or animal or plant host.
10. Organisms called slime molds have characteristics of both fungi and
ameobas.
Types of Microorganisms
PROTOZOA (S. Protozoan)
1.
Unicellular, eukaryotes microbes.
2.
Protozoa move by:
a.
Pseudopods: extensions of the cytoplasm like Ameoba.,
b.
Flagella: long appendages for locomotion like Trypanosoma.
c.
Cilia: numerous shorter appendages for locomotion like Paramecium.
3.
Protozoa have a variety of shapes.
4.
Live as free entities or as parasites (organisms
that derive nutrients from living hosts).
5.
Absorb or ingest organic compounds from their
environment)
6.
Protozoa can reproduce sexually and asexually.
Figure 1.1c
Types of Microorganisms
ALGAE (S. Alga)
1.
Photosynthetic eukaryotes
2.
Have wide variety of shapes
3.
Reproduce sexually and asexually.
4.
Unicellular and multicelluar.
5.
The cell walls of many algae, like those of plants,
are composed of cellulose (a carbohydrate).
6.
Algae are aundant in fresh and salt water, in soil, and in association with
plants.
7.
As photosynthesizers, algae need light, water, and carbon dioxide for food
production and growth.
8.
Produce molecular oxygen and organic compounds (carbohydrates) that are
used by other organisms, including animals.
9.
They play an important role in the balance of nature.
Types of Microorganisms
VIRUSES
1.
So small that can be seen only with electron microscope.
2.
Acellular (not cellular).
3.
Structurally very simple, a virus particle contains
a.
a core made only of one type of nucleic acid,
either DNA or RNA.consist of DNA or RNA core
4.
b.
The core is surrounded by a protein coat.
c.
Sometimes the coat is enclosed in a lipid envelope.
Viruses can reproduce only by using the cellular machinery of other
organisms.
5.
Obligatory intracellular parasites (replicate only when they are in a living
host cell)
Multicellular Animal Parasites
1.
Multicellular animal parasites are not strictly MOs.
2.
They are of medical importance.
3.
They are eukaryotic organisms.
4.
Multicellular animals
5.
Parasitic flatworms and round worms are called helminths.
6.
During some stages of their life cycles, helminths are microscopic in size.
Figure 12.28a
Classification of Microorganisms

Before the existence of microbes was known, all organisms were
grouped into either the animal kingdom or the plant kingdom.

In 1978, Carl Woese, devised a system of classification based on the
cellular organization of organisms.

It groups all organisms in three domains as follows:
1. Bacteria (cell walls contain a protein-carbohydrate complex called
peptidoglycan)
2. Archaea (cell walls, if present, lack peptidoglycan)
3. Eukarya, which includes the following kingdoms:
a. Protists (slime molds, protozoa, and algae)
b. Fungi (unicellular yeasts, multicellular molds, and mushrooms)
c. Plants (includes mosses, ferns, conifers, and flowering plants)
d. Animals (includes sponges, worms, insects, and vertebrates).
A Brief History of Microbiology

The science of Microbiology dates back only two hundred years.

However, microorganisms have been around for thousands of years.

Ancestors of bacteria were the first living cells to appear on Earth.

The first microbes (animalcules) were observed in 1673 by Leeuwenhoek.

In 1665, Robert Hooke reported that living things were composed of little
boxes or cells, with the help of a relatively crude
microscope.

In 1858, Rudolf Virchow said cells arise from
preexisting cells.

Cell theory: All living things are composed of cells
and come from preexisting cells.

1673-1723: Antoni van Leeuwenhoek described live
microorganisms (animalcules) that he observed in
teeth scrapings, rain water.
The Debate Over Spontaneous Generation
 After van Leeuwenhoek discovered the “invisible” world of
microorganisms, the scientific community of that time became
interested in the origins of these tiny living things.
 Not much more than 100 years ago, many scientists and philosophers
believed that some forms of life could arise spontaneously from
nonliving matter, they called this the hypothesis of spontaneous
generation.
 Therefore, people commonly believed that toads, snakes, and mice
could be born of moist soil; that flies could emerge from manure;
and that maggots, the larvae of flies, could arise from decaying
corpses.
 According to spontaneous generation, a “vital force” forms life.
 The alternative hypothesis, that the living organisms arise from
preexisting life, is called biogenesis.
Evidence PRO and CON
Redi’s Experiments
A.
In 1668: A strong opponent of SG, Francisco Redi set out to demonstrate
that maggots did not arise spontaneously from decaying meat.
1.
Redi filled two jars with decaying meat.
2.
The first was left unsealed; the flies thaid their eggs on the meat, and
the eggs developed into larvae.
3.
The second jar was sealed and, because the flies couldnot lay their eggs
on the meat, no maggots appeared.

Redi’s antagonists were not convinced; they claimed that fresh air was needed
for spontaneous generation.


Redi set up a second experiment, in which
1.
a jar was covered with a fine net instead of being sealed.
2.
No larvae appeared in the gauze-covered jar, even though air was present.
3.
Maggots appeared only when flies were allowed to leave eggs on the meat.
Redi’s results blowed the belief that large forms of life could arise from
nonlife.
Evidence Pro and Con
Needham’s and Spallanzani’s Exp.

However, many scientists still believed that small organisms such as van
Leeuwenhoek’s “animalcules” were simple enough to be generated from
nonliving material.
B.
In 1745: John Needham performed an experiment which seemed to
strengthen the SG of MOs.
1.
He heated nutrient fluids (chicken broth)
2.
Poured them into covered flasks
3.
The cooled solution were soon teeming with microorganisms.
4.
Needham claimed that microbes developed spontaneously from the
fluids.
C.
20 years later, Lazzaro Spallanzani, suggested that MOs from the air
probably had entered Needham’s solutions after they were boiled.

Spallanzani showed that nutrients fluids heated after being sealed in a
flask did not develop microbial growth.

Needham responded by claiming the “vital force” was destroyed by heat
and kept out of the flasks by the seals.
Evidence Pro and Con

The “ vital force” principle was strengthened when Anton Lavoisier showed the
importance of oxygen to life.

Therefore, Spallanzani’s observations were criticized on the grounds that
there was not enough oxygen in the sealed flasks to support microbial growth.
D.
In 1858, Rudolw Virchow challenged SG with the concept of Biogenesis, the
claim that living cells can arise only from preexisting living cells.
E.
In 1861: Louis Pasteur demonstrated that microorganisms are present in the
air and can contaminate sterile solutions, but air itself does not create
microbes.
1.
He filled several short-necked flasks with beef broth and boiled them.
2.
Some were left open and allowed to cool.
3.
In a few days, these flasks were found to be contaminated with microbes.
4.
The sealed after-boiling flasks were free of microorganisms.
5.
Pasteur reasoned that microbes in the air were the agents responsible for
contaminating nonliving matter.
The Theory of Biogenesis
1.
Pasteur next placed broth in open-ended long-necked flasks and bent the
necks into S-shaped curves.
2.
The contents of these flasks were then boiled and cooled.
3.
The broth of in the flasks did not decay and showed no signs of life.
4.
Pasteur’s S-shaped neck allowed air to pass into the flask, but trapped the
airborne MOs that might contaminate the broth.
Figure 1.3
Pasteur’s Findings
1. Pasteur showed that MOs can be present in nonliving matter- on
solids, in liquids, and in the air.
2. He demonstrated that microbial life can be destroyed by heat and
devised methods to block access of airborne MOs to nutrients.
3. These discoveries forms the basis of aseptic techniques
(techniques that prevent contamination by unwanted MOs.), which
are now the standard practice in laboratory and many medical
procedures.
4. Pasteur’s work provided evidence that MOs can not originate from
mystical forces preset in nonliving materials.
5. Scientists now believe that a form of spontaneous generation
probably did occur on primitive Earth when life first began.
6. Pasteur showed that microbes are responsible for fermentation.
The Golden Age of Microbiology

The period from 1857-1914, has been named the Golden Age of
Microbiology.

During this period, rapid advances headed by Pasteur and Robert
Koch, led to the establishment of microbiology as a science.

Beginning with Pasteur’s work, discoveries included
1.
The agents of many diseases.
2.
The role of immunity in the prevention and cure of diseases.
3.
The relationship between microbes and disease.
4.
Antimicrobial drugs
5.
Improved the techniques for microscopy and culturing
microorganisms.
6.
Development of vaccines and surgical techniques.
7.
Studying the chemical activities of microorganisms.
Fermentation and Pasteurization

At that time, many scientists believed that air converted the sugars in
beverages into alcohols.

Pasteur found instead that microbes called yeasts convert the sugars to
alcohols in the absence of air in a process called fermentation.

Fermentation is the conversion of sugar to alcohol to make beer and wine.

Souring and spoilage are caused by different MOs called bacteria.

In the presence of air, bacteria change the alcohol in the beverage into
vinegar (acetic acid).

Pasteur’s solution to the spoilage problem was to heat the beer and wine
just enough to kill most of the bacteria that caused the spoilage in a
process called pasteurization.

Pasteurization is now commonly used to reduce spoilage and kill potentially
harmful bacteria in milk as well as in some alcoholic drinks.

Showing the connection between spoilage of food and MOs was a major step
towards establishing the relationship between disease and microbes.
The Germ Theory of Disease

Until relatively recently, the fact that many kinds of diseases are related
to MOs was unknown.

Before the time of Pasteur, effective treatments for many diseases were
discovered by trial and error, but the causes of the diseases were unknown.

The realization that yeasts play a crucial role in fermentation was the first
link between the activity of a MO and physical and chemical changes in
organic materials.

This discovery alerted scientists that MOs might have similar relationships
with plants and animals- specially, that MOs might cause diseases.

This idea was known as the germ theory of disease.

Many people did not accept this theory at that time, because for centuries
disease was believed to be punishment for individual’s crimes and misdeeds.

Most people in Pasteur’s time found it inconceivable that “invisible” microbes
could travel through the air to infect plants and animals, or remain on
clothing and bedding to be transmitted from one person to another.
The Germ Theory of Disease

1835: Agostino Bassi showed that a silkworm disease was caused by a
fungus.

1865: Pasteur found that another recent silkworm disease was caused by a
protozoan.

1840s: Ignaz Semmelwise advocated hand washing to prevent transmission
of childbirth fever from one obstetrical patient to another.

1860s: Joseph Lister used a chemical disinfectant (phenol) to prevent
surgical wound infections after looking at Pasteur’s work showing microbes
are in the air, can spoil food, and cause animal diseases.

1876: Robert Koch proved for the first time that a bacterium causes
anthrax and provided the experimental steps, Koch’s postulates, to prove
that a specific microbe causes a specific disease.
Vaccination

1796: Edward Jenner found a way to protect people from smallpox almost
70 years before Koch established that microorganism causes anthrax.

He inoculated a healthy 8-years-old volunteer with cowpox virus. The person
was then protected from cowpox and smallpox.

The process was called Vaccination, derived from Latine word vacca for cow.

The protection from disease provided by vaccination or by recovery from
the disease itself is called immunity.

In about 1880, Pasteur discovered why vaccination work by working on
cholera vaccination.

Pasteur used the term vaccine for cultures of avirulent microorganisms used
for preventive inoculation.

Some vaccines are still produced from avirulent microbial strains, others
are made from killed virulent microbes, from isolated components of
virulent MOs, or by genetic engineering techniques.
The Birth of Modern Chemotherapy

Treatment of disease by using chemical substances is called chemotherapy.

Chemotherapeutic agents prepared from chemicals in the laboratory are
called synthetic drugs.

Chemotherapeutic agents produced naturally by bacteria and fungi to act
against other MOs are called antibiotics.

The success of chemotherapy is based on the fact that some chemicals are
more poisonous to MOs than to the hosts infected by the microbes.

Quinine from tree bark was long used to treat malaria.

1910: Paul Ehrlich developed the first synthetic drug, Salvarsan, to treat
syphilis. (the magic bullet!)

1930s: Several other synthetic drugs derived from dyes that could destroy
MOs were developed.

Sulfonamides (sulfa drugs) were synthesized at about the same time.
The Birth of Modern Chemotherapy

1928: Alexander Fleming discovered the first antibiotic.
 On a contaminated plate, around the mold (Penicillium) was a clear area
where bacterial growth had been inhibited.
 He observed that the Penicillium mold made an antibiotic, penicillin, that
killed S. aureus.

1940s: Penicillin was tested clinically and mass produced.

Since then, thousands of antibiotics have been discovered.

Antibiotics and other chemotherapeutic drug faces many problem:
 Toxicity to humans in practical use, specially
antiviral drugs (why ?)
 The emergence and spread of new varieties
of MOs that are resistant to antibiotics.
(due to bacterial enzymes that inactivate antibiotics,
or prevention of Abt. From entering the microbe.)
Figure 1.5
Modern Developments in Microbiology
Branches of Microbiology

Bacteriology is the study of bacteria.
 Began with the van Leeuwenhoek’s first examination of tooth scrapings.
 New pathogenic bacteria are still discovered regularly.
 Many bacteriologists, look at the roles of bacteria in food and
environment.

Mycology is the study of fungi.
 Includes medical, agricultural, and ecological branches.
 Fungal infections accounting for 10% of hospital acquired infections.

Parasitology is the study of protozoa and parasitic worms.

Recent advances in genomics, the study of all of an organism’s genes, have
provided new tools for classifying microorganisms.

Previously these MOs were classified according to a limited number of
visible characteristics.
Modern Developments in Microbiology
Branches of Microbiology

Immunology is the study of immunity.

Vaccines and interferons are being investigated to prevent and cure viral
diseases.

Vaccines are now available for numerous diseases, including measles, rubella
(German measles), mumps, chickenpox, pneumococcal pneumonia, tetanus,
tuberculosis, whooping coughs, polio, and hepatitis B.

Smallpox was eradicated due to effective vaccination and polio is expected to.

Interferons, substances produced by the body’s own
immune system, inhibit the replication of viruses and
are used to treat viral diseases and cancer.

The use of immunology to identify and classify some
bacteria according to serotypes (variants within
a species) based on certain components in the cell
walls of the bacteria, was proposed by Rebecca
Lancefield in 1933.
Figure 1.4 (3 of 3)
Modern Developments in Microbiology
Branches of Microbiology
 Virology is the study of viruses.
 In 1892, Dimitri Iwanowski reported that the organism that
caused mosaic disease of tobacco was so small that is passed the
bacterial filters.
 In 1935, Wendell Stanely demonstrated that the organism ,
called tobacco mosaic virus (TMV), was different from other
microbes, so simple, and composed of only nucleic acid core and
protein core.
 In 1940s, the development of electron microscope enabled the
scientists to observe the structure and activity of viruses in
detail.
Modern Developments in Microbiology
Branches of Microbiology

Recombinant DNA Technology:
 In the 1960s, Paul Berg inserted animal DNA into bacterial DNA and the
bacteria produced an animal protein.
 Recombinant DNA is DNA made from two different sources.
 Recombinant DNA technology, or genetic engineering, involves microbial
genetics and molecular biology.

Using microbes
 Beadle and Tatum showed that genes encode a cell’s enzymes (1942).
 Avery, MacLeod, and McCarty showed that DNA was the hereditary
material (1944).
 Lederberg and Tatum discovered that genetic material could be
transferred from one bacterium to another by conjugation (1946).
 Watson and Crick proposed a model for the structure of DNA (1953).
 Jacob and Monod discovered the role of mRNA in protein synthesis (1961).
Microbes and Human Welfare

Only minority of all MOs are pathogenic.

Microbes that cause food spoilage are also a minority.

The vast majority of microbes benefit humans, other animals, and plants in
many ways.
 RECYCLING VITAL ELEMENTS

In 1880s, Beijerinck and Winogradsky showed how bacteria help recycle vital
elements between the soil and the atmosphere.

Microbial ecology: the study of the relationship between microorganisms and
their environment.

Microorganisms recycle carbon, nitrogen, sulfur, oxygen, and phosphorus into
forms that can be used by plants and animals.

Bacteria and fungi, return CO2 to the atmosphere when decomposing organic
wastes and dead plants and animals.

Algae, cyanobacteria, and plants use CO2 to produce carbohydrates.
Microbes and Human Welfare

SEWAGE TREATMENT: Using microbes to recycle water.
 Recycling water and prevent the pollution of rivers and oceans
 Bacteria degrade organic matter in sewage (99% water), producing such
by-products as carbon dioxide, nitrates, phosphates, sulfates, ammonia,
hydrogen sulfide, and methane.

BIOREMEDIATION: Using microbes to clean up pollutants.

In 1988, microbes began used to clean up pollutants and
toxic wastes produced by various industrial processes.

Bacteria degrade or detoxify pollutants such as oil and
mercury.

In addition, bacterial enzymes are used in drain
cleaners to remove clogs

Such bioremedial microbes are Pseudomonas and
Bacillus, their enzymes used in household detergents.
UN 2.1
Microbes and Human Welfare

INSECT BEST CONTROL BY MOs
 Insect pest control is important for both agriculture and the prevention
of human diseases.
 Bacillus thuringiensis infections are fatal for many insects but harmless
to other animals, including humans, and to plants.
 The bacteria produce protein crystals that are toxic to the digestive
systems of the insects.
 The toxin gene has been inserted into some plants to make them insect
resistant.
 Microbes that are pathogenic to insects are alternatives to chemical
pesticides in preventing insect damage to agricultural crops, disease
transmission, and avoid harming the environment.
Microbes and Human Welfare

MODERN BIOTECHNOLOGY AND RECOMBINANT DNA TECHNOLOGY

Biotechnology, the use of microbes to produce foods and chemicals, is
centuries old.

Genetic engineering is a new technique for biotechnology. Through genetic
engineering, bacteria and fungi can produce a variety of proteins including
vaccines and enzymes.

Recombinant DNA techniques have been used to produce a number of
natural proteins, vaccines, and enzymes.

The very exciting and important outcome of recombinant DNA techniques is
Gene Therapy: inserting a missing gene or replacing a defective one in
human cells by using a harmless virus to carry the missing or new gene into
certain host cells.

Genetically modified bacteria are used to protect crops from insects, from
freezing, and to improve the appearance, flavor, and shelf life of fruits and
vegetables. (more: Drought resistance and temperature tolerance)
Microbes and Human Disease
NORMAL MICROBIOTA

We all live in a world filled with microbes, and we all have a variety of
microorganisms on and in our bodies.

Microbes normally present in and on the human body are called normal
microbiota, or flora.

Bacteria were once classified as plants giving rise to use of the term
flora for microbes.


This term has been replaced by microbiota.
The normal microbiota not only harmless, but also benefit us.
1.
Some protect us against disease by preventing the over-growth of
harmful microbes.
2.

Others produce useful substances such as vitamine K and B.
Unfortunately, under some circumstances normal microbiota can make us
sick or infect people we contact.
Microbes and Human Disease
INFECTIOUS DISEASES

An infectious disease is one in which pathogens invade a susceptible host,
such as a human or animal.

The pathogen carries out at least part of its life cycle inside the host, and
disease frequently results.

When a pathogen overcomes the host’s resistance, disease results.

Many mistakenly believed that infectious diseases were under control

a.
Malaria would be eradicated by killing mosquitoes by DDT.
b.
A vaccine would prevent diphtheria.
c.
Improved sanitation measures would help prevent cholera transmission.
Recent outbreaks of such infectious diseases indicates that not only they
are not disappearing, but seem to be reemerging and increasing.

In addition, a number of new diseases -Emerging infectious diseases (EID)have cropped up in recent years
Microbes and Human Disease
EMERGING INFECTIOUS DISEASES

Emerging infectious diseases (EID): are diseases that are new or changing and
are increasing or have the potential to increase in incidence in the near future.

Some factors that have contributed to the emergence of EIDs:
a.
Evolutionary changes in existing organisms.
b. The spread of known diseases to new geographic regions or populations by
modern transportation.
c.
1.
Increased human exposure to new, unusual infectious agents.
West Nile encephalitis

Caused by West Nile virus

First diagnosed in the West Nile region of Uganda in 1937

Appeared in New York City in 1999
2. Bovine spongiform encephalopathy
a.
Caused by prion
b. Also causes Creutzfeldt-Jakob disease (CJD)
c.
New variant CJD in humans is related to cattle feed from infected sheep.
Emerging Infectious Diseases
3. Escherichia coli O57:H7
a. Toxin-producing strain of E. coli
b. First seen in 1982
c. Leading cause of diarrhea worldwide
4. Ebola hemorrhagic fever
a. Caused by Ebola virus
b. Causes fever, hemorrhaging, and blood clotting
c. First identified near Ebola River, Congo
d. Outbreaks every few years.
5. Invasive group A Streptococcus
a. Rapidly growing bacteria that cause extensive tissue damage
b. Increased incidence since 1995
6. Avian influenza A (H5N1)
a. Caused by Influenza A virus (H5N1)
b. Primarily in waterfowl and poultry
c. Sustained human-to-human transmission has not occurred yet
Emerging Infectious Diseases
7. Severe acute respiratory syndrome (SARS)
a. SARS-associated Coronavirus
b. Occurred in 2002-2003
c. Person-to-person transmission
8. Cryptosporidiosis
a. Caused by Cryptosporidium protozoa
b. First reported in 1976
c. Causes 30% of diarrheal illness in developing countries
d. In the United States, transmitted via water
9. Acquired immunodeficiency syndrome (AIDS)
a. Caused by Human immunodeficiency virus (HIV)
b. First identified in 1981
c. Worldwide epidemic infecting 44 million people; 14,000 new infections daily
d. Sexually transmitted disease affecting males and females
e. In the United States, HIV/AIDS cases: 30% are female and 75% are
African American