Cellular Respiration Section 9.3 Homework: 1. In what organelle does cellular respiration take place? 2. What are the inputs to cellular respiration? What are the outputs? 3. What are the three phases of cellular respiration? What happens in each phase? 4. Where in the cell do the three phases take place? 5. Draw and label a mitochondrion. Photosynthesis Review 1. What is the purpose of photosynthesis? 2. Where does it take place? Vocabulary to know: -Cellular Respiration: the process by which mitochondria break down food molecules to produce ATP -Anaerobic: requires no oxygen -Aerobic: requires oxygen -NADH: An energy carrier molecule NAD+: Empty battery NADH: Full battery -FADH2: An energy carrier molecule FAD: Empty battery FADH2: Full battery Energy carrier molecules: ATP NADPH NADH FADH2 -Cellular Respiration: the process by which mitochondria produce ATP from food molecules -Takes place in the mitochondria Mitochondria -Cellular Respiration: the process by which mitochondria produce ATP from food molecules -Takes place in the mitochondria -Outer Membrane -Inner membrane -Matrix: the part enclosed by the inner membrane -Intermembrane space: the part between the inner and outer membrane -Cellular Respiration: the process by which mitochondria produce ATP from food molecules -Takes place in the mitochondria -Molecular formula: C6H12O6 + 6O2 6CO2 + 6H20 + energy Sugar 6 Oxygen 6 Water 6 Carbon Dioxide Photosynthetic equation: 6CO2 + 12H2O + light → C6H12O6 + 6O2 + 6H2O 6 Carbon Dioxide 1 Molecule of Sugar 12 Water 6 Water 6 Oxygen Comparison: Photosynthesis: In: Carbon Dioxide Water Energy (Light) Out: Sugar Oxygen Cellular Respiration: In: Out: Sugar Oxygen Carbon Dioxide Water Energy (ATP) -Cellular Respiration: the process by which mitochondria produce ATP from food molecules -Takes place in the mitochondria -Molecular formula: C6H12O6 + 6O2 6CO2 + 6H20 + energy -3 Stages of Cellular Respiration: Cytoplasm! 1. Glycolysis A process that splits glucose into two molecules of pyruvic acid Mitochrondrial Matrix! Inner mitochondrial membrane 2. Citric Acid Cycle Produces ATP, NADH, and FADH2 that are used in the electron transport chain 3. Electron Transport Chain: produces bulk of ATP Homework: 1. In what organelle does cellular respiration take place? 2. What are the inputs to cellular respiration? What are the outputs? 3. What are the three phases of cellular respiration? What happens in each phase? 4. Where in the cell do the three phases take place? 5. Draw and label a mitochondrion. Classwork: 1. In what organelle does cellular respiration take place? The mitochondria 2. What are the inputs to cellular respiration? What are the outputs? In: Oxygen, sugar. Out: CO2, H2O, energy 3. What are the three phase of photosynthesis? What happens in each phase? Glycolysis (splits sugar into pyruvate), Citric acid cycle (uses Acetyl CoA to make NADH, FADH2, and ATP), ETC (makes a lot of ATP) 4. Where in the cell do the three phases take place? G: Cytoplasm. CAC: matrix. ETC: inner mitochondrial membrane 5. Draw and label a mitochondrian. Terms to know for the cellular respiration vocab quiz: Mitochondrion Cellular respiration Anaerobic Aerobic Matrix Intermembrane space Glycolysis Citric Acid Cycle Where glycolysis takes place Where the citric acid cycle takes place Where the electron transport chain takes place Terms to know for the cellular respiration vocab quiz: -Mitochondrion: the organelle in which cellular respiration takes place -Cellular respiration: the process by which the cell releases energy from food molecules -Anaerobic: requires no oxygen -Aerobic: requires oxygen -Matrix: the space inside the inner membrane of the mitochondrion -Intermembrane space: the space between the inner and outer membranes of the mitochondrion -Glycolysis: (1st stage of cellular respiration) the process of splitting glucose into two molecules of pyruvic acid -Citric acid cycle: (2nd stage of cellular respiration) the process of producing ATP, NADH, and FADH2 which will be used to fuel the electron transport chain (3rd stage of cellular respiration) -Where glycolysis takes place: cytoplasm (outside the mitochondrion) -Where the citric acid cycle takes place: mitochondrial matrix -Where the electron transport chain takes place: the inner membrane Glycolysis/ Cellular Respiration Quiz Questions 1. What is glycolysis? 2. What are the products of glycolysis? 3. Where does glycolysis take place? *4. What is pyruvate decarboxylation? *5. Where does pyruvate decarboxylation take place? 6. What are the products of the citric acid cycle? 7. What are the total products of glycolysis, *pyruvate decarboxylation, and the citric acid cycle? 8. Where do these products go? STEPS OF CELLULAR RESPIRATION: 1. Glycolysis: A process that splits glucose into two molecules of pyruvate -Produces 2 net ATP and 2 NADH Terms to know for the cellular respiration vocab quiz: Mitochondrion: the organelle in which cellular respiration takes place Cellular respiration: the process by which the cell releases energy from food molecules Anaerobic: requires no oxygen Aerobic: requires oxygen Matrix: the space inside the inner membrane of the mitochondrion Intermembrane space: the space between the inner and outer membranes of the mitochondrion Glycolysis: (1st stage of cellular respiration) the process of splitting glucose into two molecules of pyruvic acid Citric acid cycle: (2nd stage of cellular respiration) the process of producing ATP, NADH, and FADH2 which will be used to fuel the electron transport chain (3rd stage of cellular respiration) Where glycolysis takes place: cytoplasm (outside the mitochondrion) Where the citric acid cycle takes place: mitochondrial matrix Where the electron transport chain takes place: the inner membrane Mitochondrion Quiz 5. Label 6. Define 1. 2. 3. Label 4. Define Mitochondrion Quiz 1. Outer membrane 2. Inner membrane 5. Matrix 6. Space inside the inner membrane 3. Intramembrane space 4. Space between inner and outer membranes 1. Glycolysis: A process that splits glucose into two molecules of pyruvate Glucose: 6 carbon molecule Pyruvate: 3 carbon molecule -Glycolysis uses up 2 ATP and generates 4 ATP. Therefore, glycolysis produces 2 net ATP (and 2 NADH) 1. Glycolysis: A process that splits glucose into two molecules of pyruvic acid -Produces 2 net ATP and 2 NADH -Glycolysis is an anaerobic process -Takes place in the cytoplasm 1.5: Pyruvate decarboxylation: Pyruvate is shipped to the intermembrane space where it is converted to a molecule called Acetyl CoA This process produces 2 NADH Mitochondria MATRIX Citric Acid Cycle Pyruvate Acetyl CoA Glycolysis Cytoplasm INTERMEMBRANE SPACE 2. Citric acid cycle: a cycle that adds Acetyl CoA to other molecules to produce 3 NADH, 1 ATP, and 1 FADH2 2. Citric acid cycle: a cycle that adds Acetyl CoA to other molecules to produce 3 NADH, 1 ATP, and 1 FADH2 Acetyl CoA Oxaloacetate 2. Citric acid cycle: a cycle that adds Acetyl CoA to other molecules to produce 3 NADH, 1 ATP, and 1 FADH2 per Acetyl CoA -Note that glycolysis yields 2 pyruvate, and each pyruvate is converted to an Acetyl CoA. -Therefore, for each molecule of glucose, the Citric Acid Cycle produces 6 NADH, 2 ATP, and 2 FADH2 2. Citric acid cycle: a cycle that adds Acetyl CoA to other molecules to produce 3 NADH, 1 ATP, and 1 FADH2 per Acetyl CoA -Note that glycolysis yields 2 pyruvate, and each pyruvate is converted to an Acetyl CoA. -Therefore, for each molecule of glucose, the Citric Acid Cycle produces 6 NADH, 2 ATP, and 2 FADH2 Pyruvate C C C Acetyl CoA C C 3 NADH 1 ATP 1 FADH2 Glucose C C C C C C Pyruvate C C C Acetyl CoA C C 3 NADH 1 ATP 1 FADH2 Products so far: Citric Acid Cycle Glycolysis 2 NADH 2 net ATP Pyruvate Acetyl CoA 2 NADH TOTAL: 10 NADH 4 ATP 2 FADH2 Become the inputs for the electron transport chain 6 NADH 2 ATP 2 FADH2 Glycolysis/ Cellular Respiration Quiz Questions 1. What is glycolysis? 2. What are the products of glycolysis? 3. Where does glycolysis take place? 4. What is pyruvate decarboxylation? 5. Where does pyruvate decarboxylation take place? 6. What are the products of the citric acid cycle? 7. What are the total products of glycolysis, pyruvate decarboxylation, and the citric acid cycle? 8. Where do these products go? 1. What is glycolysis? A process that splits glucose into two molecules of pyruvic acid 2. What are the products of glycolysis? 2 NADH, 2 ATP (2 pyruvates) 3. Where does glycolysis take place? cytoplasm 4. What is pyruvate decarboxylation? Pyruvate -> Acetyl CoA 5. Where does this conversion take place? Intermembrane space 6. What are the products of the citric acid cycle? 6 NADH, 2 ATP, 2 FADH2 7. What are the total products of glycolysis, pyruvate decarboxylation, and the citric acid cycle? 10 NADH, 4 ATP, 2 FADH2 8. Where do these products go? They will be used in the electron transport chain Products so far: Citric Acid Cycle Glycolysis 2 NADH 2 net ATP Pyruvate Acetyl CoA 2 NADH TOTAL: 10 NADH 4 ATP 2 FADH2 Become the inputs for the electron transport chain 6 NADH 2 ATP 2 FADH2 3. Electron Transport Chain: provides bulk of the ATP (38) -In the ETC, each NADH produces 3 ATP. Each FADH2 produces 2 ATP. 3. Electron Transport Chain: provides bulk of the ATP (38) -In the ETC, each NADH produces 3 ATP. Each FADH2 produces 2 ATPS. GLYCOLYSIS/C.A. CYCLE: becomes 10 NADH 4 ATP 2 FADH2 ATP PRODUCED IN THE ETC 30 ATP 4 ATP 4 ATP Total ATP produced by cellular respiration: 38 ATP H+ Intermembrane Space H+ H+ Electron Transport Chain H+ H+ H+ H+ H+ H+ Matrix H+ H+ H+ H+ Intermembrane Space H+ H+ H+ H+ H+ E- E- H+ NADH FADH2 NAD+ FAD H+ H+ Matrix H+ H+ H+ H+ Intermembrane Space H+ H+ H+ H+ H+ E- E- H+ NADH FADH2 NAD+ FAD H+ H+ Matrix H+ H+ H+ H+ Intermembrane Space H+ H+ H+ H+ H+ EE- H+ NADH FADH2 NAD+ FAD H+ H+ Matrix H+ H+ H+ H+ Intermembrane Space H+ H+ H+ H+ H+ EE- H+ NADH FADH2 H+ H+ NAD+ FAD H+ Matrix H+ H+ H+ Intermembrane Space H+ H+ H+ H+ H+ H+ E- E- NADH FADH2 NAD+ FAD H+ Matrix H+ H+ H+ H+ H+ Intermembrane Space H+ H+ H+ H+ H+ H+ H+ H+ E- E- NADH FADH2 NAD+ FAD H and O2 H+ Matrix H+ H+ H2O H+ Intermembrane Space H+ H+ H+ H+ H+ H+ H+ H+ E- E- NADH FADH2 NAD+ FAD H and O2 H+ Matrix H+ H+ H2O Mitochondria MATRIX Pyruvate Acetyl CoA Glycolysis INTERMEMBRANE SPACE Mitochondria MATRIX H+ H+ H+ H+ + H+ H H+ ETC INTERMEMBRANE SPACE Mitochondria H+ H+ H+ H+ MATRIX + H+ H H+ INTERMEMBRANE SPACE Mitochondria H+ H+ MATRIX + H H+ H+ H+ H+ INTERMEMBRANE SPACE Mitochondria H+ H+ MATRIX ATP Synthase + H H+ H+ H+ H+ INTERMEMBRANE SPACE Mitochondria H+ H+ MATRIX + H H+ H+ H+ H+ INTERMEMBRANE SPACE Mitochondria H+ H+ MATRIX H+ H++ H H+ H+ INTERMEMBRANE SPACE Mitochondria MATRIX H+ H+ H+ H+ H+ H H+ + ATP INTERMEMBRANE SPACE Mitochondria MATRIX H+ H+ H+ H H+ + ATP H+ H+ ATP ATP ATP ATP ATP INTERMEMBRANE SPACE Mitochondria MATRIX H+ H+ H+ H+ H+ H H+ + (38 ATP) ATP ATP ATP ATP ATP ATP INTERMEMBRANE SPACE ETC Quiz Questions 1. What provides the electrons for the electron transport chain? 2. How many ATPs does one molecule of NADH make? 3. How many ATPs does one molecule of FADH2 make? 4. What is the total number of ATPs produce by cellular respiration? 5. Why is it important for the cell to make ATP? 1. What supplies the electrons for the electron transport chain? NADH, FADH2 2. How many ATPs does one molecule of NADH make? 3 3. How many ATPs does one molecule of FADH2 make? 2 4. What is the total number of ATPs produce by cellular respiration? 38 5. What does the cell do with ATP? Use it for cell functions Cellular Respiration Quiz Thursday What is the point of cellular respiration? Where does cellular respiration take place? Draw and label a mitochondrion. What are the three phases of cellular respiration? What happens in each phase of cellular respiration? What are the inputs and outputs of cellular respiration? Where do animal cells get sugar for cellular respiration? Where do plant cells get sugar for cellular respiration? Cellular Respiration Quiz 1. Draw and label a mitochondrion. Include the following terms: inner membrane, outer membrane, christae, matrix, intramembrane space 2. What are the three phases of cellular respiration? 3. What happens in each phase of cellular respiration? 4. What are the inputs and outputs of cellular respiration? Molecular version of CR https://www.youtube.com/watch?v=-Gb2EzF_XqA Bozeman photosynthesis/cellular respiration http://www.youtube.com/watch?v=0IJMRsTcwcg&feature=related Photosynthesis and Cellular Respiration Review 1. Where do plant cells get their food? 2. What is the point of photosynthesis? 3. Where does photosynthesis take place? 4. Draw and label a chloroplast. What are the two phases of photosynthesis? What happens in each phase of photosynthesis? What are the inputs and outputs of photosynthesis? What is the point of cellular respiration? Where does cellular respiration take place? Draw and label a mitochondrion. What are the three phases of cellular respiration? What happens in each phase of cellular respiration? What are the inputs and outputs of cellular respiration? Where do animal cells get sugar for cellular respiration? Where do plant cells get sugar for cellular respiration? Is cellular respiration aerobic or anaerobic? Lactic Acid Fermentation Lab Quiz 1. What caused the burning sensation in the subject’s muscles? 2. How do we know that the subject’s muscles were using fermentation? 3. Why did the subject’s muscles rely on fermentation instead of cellular respiration? 4. What are the advantages of cellular respiration? 5. What are the advantages of fermentation? Lactic Acid Fermentation Lab Quiz 1. What caused the burning sensation in the subject’s muscles? The build up of lactic acid 2. How do we know that the subject’s muscles were using fermentation? There was lactic acid present in the subject’s muscles 3. Why did the subject’s muscles rely on fermentation instead of cellular respiration? The subject’s muscles were using oxygen at a faster rate than what his/her body could provide, so cells had to use anaerobic respiration to provide ATP 4. What are the advantages of cellular respiration? Makes more ATP 5. What are the advantages of fermentation? Doesn’t need oxygen to make ATP