CATALYST (11.12.2008) Take out your quiz from Monday and trade with the person sitting in front of you. If you are in the front row, take your paper to the back of the classroom. Begin working on your quiz, you have 15 minutes to complete it. Once you have completed your quiz return it to the creator to be graded. Turn in all graded quizes. CATALYST (11.13.2008) “The aim of this research [at the University of Leicester] is to understand how potential jurors perceive and understand various forensic science techniques, in order to determine how their attitudes and expectations may impact on their decision making in a courtroom setting.” “Forensic science is featured quite heavily in television programming and news media coverage, and this raises the public interest in forensic techniques. An important aspect of jury decision making is a juror's prior beliefs and knowledge, and this study aims to investigate how potential jurors' perceptions of forensic science impacts on their ability to evaluate different types of evidence.” How do you think shows like CSI affect a juror’s ability to make judgments in a case? DNA FINGERPRINTING SIMULATION LAB 7.3: OBJECTIVES Assume the role of a forensic scientist in an attempt to solve a violent crime using DNA evidence. Employ agarose electrophoresis to separate digested DNA fragments according to size. Identify the guilty suspect based on the DNA fingerprinting evidence. Understand the principles and practices of DNA technology as applied to the forensic sciences. LAB 7.3: SCENARIO Police were called to the scene of a homicide. Found at the scene was a large amount of blood. Blood typing revealed that not only was the blood of the victim present at the scene, but also blood from another person, assumed to be the guilty party. Blood evidence was collected at the scene. Blood samples from four suspects were also drawn under court order and all samples were found to be of the same blood type as the blood believed to have come from the perpetrator of the crime. LAB 7.3: SCENARIO The police have turned to you and your fellow lab technicians to employ the process of DNA fingerprinting in building a case against one of the suspects. LAB 7.3: PRE-LAB QUESTIONS 1. 2. 3. 4. 5. 6. Summarize the scenario in one sentence. Why were the police unable to use blood type to identify the perpetrator? What type of DNA will be analyzed on the gel? What is electrophoresis? What property does DNA posses that allows us to visualize banding patterns using electrophoresis? How do we get enough DNA from blood samples to analyze using gel electrophoresis? What safety precautions should be taken before beginning this lab? LAB 7.3: PROCEDURES Create a quick Guide for the 9 procedures found in the text on page 186. (This may become homework if you do not finish). Sketch a DNA “gel” in lab notebook to record your results. Label each well with the sample that will be placed there. LAB 7.3: CONCLUSION QUESTIONS 1. 2. 3. Which suspect’s DNA profile matches the crimescene DNA evidence? Justify your answer, referring to the DNA fingerprint and the DNA standard marker as a reference. Explain what other testing could be done using DNA gel electrophoresis to increase the probability that a person's DNA either matches or does not match the evidence? REST OF CLASS PERIOD Finish the Chapter 7 Review at the back of the chapter you will get a study guide for the DNA Fingerprinting Quest tomorrow. If you have time read two of the case studies at the back and summarize them in 2 sentences in your notebook.