SYLLABUS
CHM 366
Spring 2009
Introductory Biochemistry Laboratory
Instructor:
Dr. Leslie Meadows Frost
464 Science
Phone: 696-6774
e-mail: Frost@marshall.edu
e-mail is the best way to contact me.
Office Hours:
I am always happy to assist at any time when I am in my office. Official office hours are: T,R 1-3.
Statement of Course:
Introduction to basic biochemical laboratory techniques including chromatography, electrophoresis,
methods for identification and characterization of biochemical systems, and protein and DNA analysis.
Attendance Policy: Attendance is mandatory for this course. You must be present to perform each lab each week. There are no
make-up labs. If you have a University excuse, then arrangements will be made to compensate for that particular lab.
Textbook:
each lab.
There is no textbook for this course. We will be using handouts that I will give to you at least one week prior to
Schedule:
Date
Jan. 14
Jan 21
Jan 28
Feb 4
Feb 11
Feb 18
Feb 25
March 4
March 11
March 18
March 25
April 1
April 8
April 15
April 22
April 29
Experiment
Check-in, lab safety
Acid-Base Properties of Amino Acids
Determining Protein Concentration
Size Exclusion chromatography
ELISA HIV assay
Comparative proteomics
Comparative proteomics
Comparative proteomics
Enzyme Part I
Enzyme Part II
Spring Break
Peptide Analysis
No Class
DNA Informatics
DNA Fingerprinting
TBA
Reports Due
Acid-Base Report Due
Protein Concentration Report Due
Size Exclusion Report Due
ELISA Report Due
Comparative Proteomics Report I Due
Comparative Proteomics Report II Due
Enzyme Report Due
DNA Fingerprinting Report Due
Grading Scale:
90-100
80-89
70-79
60-69
Below 59
A
B
C
D
F
Lab Reports
Instructor Evaluation
90%
10%
You will be given a grade for each experiment based upon results and written laboratory reports. For most of the experiments, a short
write up will be required that contains the pertinent information about the lab and all results and conclusions. In addition, several of
the labs will have questions that must be answered. We will be writing a few formal lab reports. A handout will be supplied which
will contain the basics for writing a formal lab report.
Notebook:
You will need a hard cover lab notebook for the recording of data and observations.
The key to doing well in this lab is to work hard and take the time to learn the key concepts presented in each lab. This will be an
informal lab with an emphasis on learning the material, not on how well you can write-up a 30 page lab report. I hope everyone will
have fun in the lab and learn some valuable biochemical techniques.
Academic Dishonesty: Students should read the following section from the Marshall catalog which describes academic dishonesty
(http://www.marshall.edu/www/ugradcat/PDF/05_09.pdf). Any student caught cheating in this course will receive 0 points on that
assignment or exam.
“Policy for Students with Disabilities: Marshall University is committed to equal opportunity in education for all students, including
those with physical, learning and psychological disabilities. University policy states that it is the responsibility of students with
disabilities to contact the Office of Disabled Student Services (DSS) in Prichard Hall 117, phone 304 696-2271 to provide
documentation of their disability. Following this, the DSS Coordinator will send a letter to each of the student’s instructors outlining
the academic accommodation he/she will need to ensure equality in classroom experiences, outside assignment, testing and grading.
The instructor and student will meet to discuss how the accommodation(s) requested will be provided. For more information, please
visit http://www.marshall.edu/disabled or contact Disabled Student Services Office at Prichard Hall 11, phone 304-696-2271.”