Remarques intéressantes Article de Friedman : prognostic of initial endodontic therapy Also, intracanal medicaments used in the studies may have been ineffective. Intracanal medicaments are critical for controlling root canal infection (29,56–61), but not all are equally effective. The antimicrobial efficacy of ‘classical’ medicaments, such as camphorated phenol and paramonochlorophenol, iodine potassium iodide and formocresol, is shortlived (62–64), and may be insufficient for in-between-sessions disinfection of canals associated with apical periodontitis (59). These ‘classical’ medicaments have been used in many studies (3, 9–12, 14, 15, 17–19, 24), where they may have compromised the results. The more effective calcium hydroxide. Healing of apical periodontitis is a dynamic process, and sufficient time is required to evaluate its progression and completion (1, 29, 42). Observations after a short follow-up may demonstrate only signs of healing (1, 37, 40, 42) (Fig. 4). Therefore, results of studies with short follow-up periods (Table 1) may be skewed and not reflect the true prognosis (1, 54, 55, 71). Follow-up of at least 1year is required to reveal meaningful changes (34, 42), but extension of the follow-up to 3 or 4years (Fig. 5) may be required to record a stable treatment outcome (1, 17, 29, 42, Because with time, endodontically treated teeth are subject to adverse effects of periodontal and restorative deterioration, extensive follow-up periods are more likely to reveal the influence of those effects on the outcome. Comparing the 4-year and the final follow-up, Strindberg (1) observes a difference in healing rates of 16%. Clearly, then, the poorer prognosis can be ‘blamed’ on the infection, but not necessarily on the overfilling. The goal of initial endodontic therapy is to cure apical periodontitis (80). When radiolucency is still present at follow-up, it is an expression of apical periodontitis the same disease the initial therapy aimed to cure. However, it should be taken into account that, because all endodontically treated teeth remain constantly challenged by intraoral microorganisms, development of apical periodontitis in the future remains a possibility for all teeth, even those that are completely healed at one point after therapy. Therefore, periodic follow-up of endodontically treated teeth is advocated as a viable routine. Persistance de la maladie = le plus souvent due à une infection résiduelle du système canalaire. Mais l’extrusion de matériel étranger et la présence de kystes peuvent mimer une vraie parodontite apicale. Mais cela est peu fréquent. Une parodontite apicale persistante est tout d’abord causée par une infection (première cause !), Les sites microbiens peuvent être différents : 1. 2. Le plus souvent les MO sont présents dans le système canalaire malgré le traitement ou viennent envahir les canaux après traitement (restauration coronaire non étanche). Des Mo spécifiques peuvent s’établir dans les tissus péri-apicaux (actinomyces israelii, Arachnia propionica) 3. MO d’autres espèces à l’extérieur du système canalaire, dans les tissus péri-apicaux, sur la surface radiculaire, dans des lacunes du cément, dans des biofilms microbiens ressemblant à de la plaque ou sur des débris de dentine extériorisés lors du traitement. Retenir que la cause principale est l’infection radiculaire ! Friedman = la parodontite apicale is « universally considered a disease requiring therapy », qu’il y ai ou pas des symptômes. De la même manière une parodontite apicale persistante après traitement ne peut pas être considérée comme un succès seulement parce que la dent est asymptomatique : « it is the same disease, still requiring management ». La guérison est supposée être totale. Donc tout reste de radiotransparence doit être considéré comme une parodontite apicale persistante ou récurrente. Cependant la guérison de lésions péri-apicales de grandes étendues peuvent laisser des cicatrices fibreuses (persistance d’une radiotransparence). Succès clinique = disparition des symptômes et plus grande liberté quant au cliché radiographique Une définition plus stricte impose un succès clinique et radiographie (radiotransparence acceptée seulement si présente autour de l’éjection de matériel). Le problème de la définition la moins contraignante est qu’une parodontite apicale est fréquemment asymptomatique (2, 26 ; 47, 46) Toronto study, phase 1 : The quality of a clinical study is primarily concerned with validity and relevance (3). The quality parameters can be grouped into four categories as follows: (a) Cohort: this should be defined at the inception of the study and clearly described. The pattern of referral and case-selection criteria should be described (4). At the end point of the study, the entire inception cohort should be accounted for (4). The sample size may be required to exceed a certain threshold. (b) Exposure (intervention, treatment): treatment providers and procedures should be clearly described. Procedures considered to be irrelevant or unacceptable may be excluded. (c) Outcome assessment: outcome dimensions (5) should be clearly defined. Measures used to assess these dimensions should be objective and applied consistently by properly calibrated examiners with established reliability. The examiners should be blinded or masked and different from the providers of treatment. Direct visual comparisons of radiographs, e.g. preoperative and at follow-up, should be avoided. The follow-up period should be long enough to capture the completion of the healing process in the majority of the study sample. (d) Data analysis and reporting: potentially confounding prognostic factors should be controlled, or at least observed, and recorded. Being frequently related to traumatic occlusion, food impaction, or periodontal disease, tenderness to percussion was allowed if unaccompanied by any other clinical sign or symptom The fact that state-of-the-art treatment did not dramatically improve the outcome once more highlighted the complexity of treating apical periodontitis. Apparently, prevention or treatment of this disease cannot be improved merely by changing treatment techniques. Because apical periodontitis results from interactions between microorganisms, their environment and the host immune system (19), only use of effective modifiers of any of these three factors mightsignificantly improve the outcome of treatment. Toronto study, phase 2 : The results regarding initial treatment for Phase I (17) revealed that the outcome (81% overall healed rate) was significantly better in teeth treated without the presence of AP. With AP present, the outcome was significantly better for singlerooted than for multirooted teeth. Several factors were associated with a large (_10%), albeit statistically nonsignificant healing rate differential. Two factors were of particular interest: (a) number of treatment sessions in teeth with AP; and (b) treatment technique: flared canal preparation and vertical compaction of warm guttapercha (FPVC) as described by Schilder (18, 19), or modified step-back preparation and lateral compaction of gutta-percha (SBLC) (20 –22). The lack of significance for some of those factors is reflective of insufficient power because of the limited sample size of the Phase I study (17). Based on the Phase I study results (17), it was hypothesized that the healed rate would be significantly higher for: (a) single-rooted than multirooted teeth; (b) treatment using FPVC than SBLC technique; and (c) treatment of teeth presenting with preoperative AP in two or more sessions than in one session. reflect absence (PAI ≤ 2) or presence (PAI ≥3) of AP. Outcome assessment was based on clinical and radiographic measures, and the periapical tissues classified as “healed” (absence of AP, signs, and symptoms other than tenderness to percussion), or as having “disease” (presence of AP, signs, or symptoms). Teeth presenting without clinical signs or symptoms were considered “functional” regardless of the PAI score The bivariate analysis (Table 2) identified only two statistically significant associations with a higher healed rate for treatment without preoperative AP than with AP, and treatment using FPVC than SBLC. Five additional factors were associated with “large” (_10%) healed rate differentials that were not statistically significant. Of 70 teeth treated with preoperative AP, 57 (81%) had healed. Stratified analysis (Table 4) identified only one statistically significant association with a higher healed rate for treatment using FPVC than SBLC. Eight additional factors were associated with large healed rate differentials that were not statistically significant. Thus, the absence of symptoms is insufficient as a measure of healing. Patients should be encouraged to attend followup examinations after endodontic treatment to assess the outcome, even if they are asymptomatic. Nevertheless, absence of symptoms does allow the tooth to remain “functional.” This dimension of outcome should not be overlooked when communicating to patients the benefits associated with endodontic treatment visà-vis tooth extraction and replacement. The healed rate (combined sample, 85%) differed significantly for preoperative AP (absent, 93%; present, 79%), treatment technique (flared preparation and vertical compaction, 90%; stepback preparation and lateral compaction, 80%), gender (females, 90%; males, 79%), number of roots (1– 92%; >2–81%), and root-filling length (adequate, 87%; inadequate, 77%). Logistic regression revealed increased risk of disease for preoperative AP (odds ratio _ 3.3) and technique (odds ratio _ 2.3). This study confirmed AP and highlighted treatment technique as the main predictors of outcome in initial treatment. It is noteworthy that 45% (combined sample) of the teeth having disease at follow-up demonstrated reduced lesions relative to the preoperative size. Although size reduction does not suggest that the lesion has healed, it may be a sign of slowprogressing healing. In a recent study (28), as many as 6% of teeth that had persistent AP 10 yr after treatment have been observed to completely heal 10 to 17 yr later. This finding should be communicated to patients to emphasize the need for extended follow-up as long as complete healing has not been observed. Clearly then, the significant effect of the treatment technique on outcome was specific to teeth with preoperative AP. The FPVC technique, as taught in the Graduate Endodontics Clinic at the University of Toronto, strictly adhered to the original description by Schilder of the cleaning and shaping protocol (19) and vertical compaction of warm guttapercha (18) using Kerr Pulp Canal Sealer (Kerr, Romulus, MI). The main alternative technique taught (SBLC) comprised stepback cleaning and shaping (20), often modified to include extensive apical reaming (21), and lateral compaction of guttapercha (22) with Roth’s 801 sealer Thus, the technique described by Schilder (18, 19), as a complete concept, yielded a better outcome than SBLC. However, because this study was not designed specifically to compare the two treatment techniques, e.g. it was not a randomized, controlled trial, the result can only be considered as suggestive. The outcome differed for single-rooted and multirooted teeth, as previously observed in the Phase I study (17) only for teeth with preoperative AP. Whether reflecting the complexity of eliminating root canal infection in the multirooted teeth or just the use of the tooth as the unit of evaluation (5), this finding suggested a practical application: clinicians can better appraise patients of the prognosis by citing the expected healing rate for the specific tooth type, rather than an average healing rate for single-rooted and multirooted teeth. It should be noted, however, that this finding was contrary to that of Strindberg (6) and Engström et al. (7). Gender has not been found to significantly affect the outcome in the selected studies (11, 17). The potential confounding effect of other factors on this finding was assessed and excluded (tests not shown), and no explanation could be offered for this observation. Indeed, the largest healing rate differential related to root-filling length (15%) was observed among teeth with AP. It has been suggested that this adverse effect could be a result of over-instrumentation and subsequent transportation of contaminated debris periapically (5, 11, 34), rather than the periapical extrusion of the root-filling material per se. Toronto Study phase 3 Logistic regression performed on the combined phases I-III sample identified significant (p _ 0.05) outcome predictors: preoperative AP (OR _ 3.5; CI 1.7-7.2; healed: absent, 93%; present, 80%), number of roots (OR _ 2.2; CI 1.0-4.7; healed: 1 - 92%; _2 - 83%), and intraoperative complications (OR _ 2.2; CI 1.1-4.5; healed: absent, 88%; present, 76%). Treatment technique (OR _2.8; CI 1.3-6.1; healed: Schilder, 89%; alternative, 73%) was suggested as an outcome predictor in teeth with AP, requiring confirmation from randomized controlled trials. Toronto Study phase 4 When pooled with Phases 1–3, 439 of 510 teeth (86%) were healed. Logistic regression identified 2 significant (P_ .05) preoperative outcome predictors: radiolucency (odds ratio [OR], 2.86; confidence interval [CI], 1.56 –5.24; healed: absent, 93%; present, 82%) and number of roots (OR, 2.53; CI, 1.25–5.13; healed: single, 93%; multiple, 84%). In teeth with radiolucency, intraoperative complications (OR, 2.27; CI, 1.05– 4.89; healed: absent, 84%; present, 69%) and root-filling technique (OR, 1.89; CI, 1.01– 3.53; healed: lateral, 77%; vertical, 87%) were additional outcome predictors. A better outcome was suggested for teeth without radiolucency, with single roots, and without mid-treatment complications. The predictive value of root-filling technique in teeth with radiolucency requires validation from randomized controlled trials. Preoperative apical periodontitis was identified as an outcome predictor in all 3 phases (19, 22, 25), as in the majority of previous studies (6, 7, 13, 18, 21, 23, 24). More than 1 root in the treated tooth was identified in the latter 2 phases (22, 25), and occurrence of a mid-treatment complication was identified in the most recent phase (25). The multivariate analysis (Table 4) identified 2 preoperative significant predictors of disease persistence, presence of radiolucency (odds ratio [OR], 2.86), and 2 or more roots (OR, 2.53). Stratified multivariate analysis of the subsample of teeth with preoperative radiolucency (Table 5) identified 2 significant predictors of disease, midtreatment complications (OR, 2.27) and root-filling by using LC (OR,1.88). Therefore, patients who are considering initial treatment and alternative extraction and replacement should be informed of the 86% chance for the treated tooth to heal completely, an additional 5% chance for incomplete healing, and 95% chance for functional retention 4 – 6 years after initial treatment. Indeed, in a study spanning 27 years of follow-up (32), approximately 6% of teeth appeared healed only during the second or third decade after treatment. The finding of a better healed rate in single-rooted teeth than in multi-rooted teeth (one, 93%; two or more, 84%) was not surprising, considering that the evaluated unit was the tooth, not the individual root. In multi-rooted teeth, the risk for persistence of disease is proportional to the number of roots. Therefore, the results might have been inherent to the method of analysis, but they might also reflect the greater challenge encountered when the anatomically complex, multi-rooted teeth are treated. In any event, clinicians should consider the 11% difference in outcome when projecting the prognosis for single-rooted and multirooted teeth. By their nature, these complications can either promote infection or interfere with its elimination; therefore, iatrogenic complications should be avoided to maximize the outcome of treatment in teeth with apical periodontitis. Vytaute Peciuliene, Jurate Rimkuviene, Rasma Maneliene, Deimante Ivanauskaite : Apical periodontitis in root filled teeth associated withthe quality of root fillings. Stomatologija, Baltic Dental and Maxillofacial Journal, 8:122-6, 2006 Periapical Index (PAI) established by Orstavik et al. based on the histological work of Brynolf allows standardization of the different categories, and thus comparisons between studies [9, 12]. Its reliability was established by further investigations [2, 3, 4]. However, in order to differentiate the normal status from the pathologic, the authors proposed a cut-off at a score of 2, since PAI>2 was considered to be indicative of periapical pathology. Score of 2 corresponds to an image with a localized widening of the ligament and can be associated signs of bone modifications which may be interpreted as an ongoing healing process, an established state of irritation, or an evolution toward a pathological state. Since peak incidence of healing or emerging chronic apical periodontitis is at 1 year, the risks of a questionable image developing a more advanced lesion are increased [1]. Periapical lesions are in general radiographically underestimated, since the cortical bone must have a 30-50% mineral bone loss to be detectable [13]. Moreover, as the PAI system was established for maxillary anterior incisors, where the cortical bone is thin, the risk of underestimation of lesions with a PAI>2 is increased. A comparison of the quality of root canal treatment in two Danish subpopulations examined 1974–75 and 1997–98 The periapical status of root-filled teeth depends on both the quality of the root canal treatment and of the coronal restoration (Ray & Trope 1995, Kirkevang et al . 2000b). Periapical bone was judged sound if the PAI score was 1 or 2 and diseased if the PAI score was 3, 4 or 5 (Table 1) The quality of the lateral seal in the two groups is shown in Table 6. Only about one-quarter of the teeth in group 1 had adequately sealed root canals, whereas in group 2 almost half of the teeth were judged adequately sealed. The difference was highly significant (P < 0.001). In Table 7, the length of the root filling in the two groups is shown. Less than half of the root fillings in group 1 had adequate length, whereas in group 2 more than half of the root fillings were judged adequate (P <0.001). The rate of pulpotomies in group 1 was 5.3%, and in group 2 it was 1.1% (P< 0.001). The periapical status of the two groups is shown in Table 8. More root fillings appeared to be adequate during 1997–98 than during 1974–75, and fewer pulpotomies were found in 1997–98. The quality of the root fillings during 1997–98 was found to be at the same level as in a study of randomly selected individuals living in Aarhus County (Kirkevang et al. 2000b). This supports the assumption that the technical quality of endodontic treatment is less patient dependent. Another explanation could be a change in the treatment strategy of apically infected teeth toward more conservative treatments, where dentists hesitate to extract teeth with periapical lesions, but extend the period of conservative treatment and try to treat/retreat them. This would result in a higher rate of ‘diseased’ or ‘dubious’ teeth in a population. Other studies have found a slight improvement in periapical status over time (Eckerbom et al. 1989, Petersson 1993). The fact that the periapical status of root-filled teeth in the present study had not improved with the better quality treatment indicates that the quality of the root fillings is not the only parameter that influences the periapical status. The quality of the root filling, as depicted by radiography, may be of importance for the outcome of the treatment, but it does not provide any information on microbial contamination that will probably play an important role in the outcome Although the two populations under comparison were not identical with respect to disease frequency, the technical quality of root canal treatment had improved from 1974 to 1997 Endodontic infection: Some biologic and treatment factors associated with outcome Optimal prognosis for endodontic treatment is dependent on the successful elimination of microorganisms from the infected root canals.1-5 To optimize successful outcome, the focus of endodontic treatment is directed at disinfection through chemomechanical debridement and biomechanical preparation, followed by a quality obturation of the root canal system. Finally, it is necessary to preserve the remaining tooth structure by timely placement of an appropriate permanent restoration. Complete disinfection of the infected pulp space is difficult, and there is still no predictable, effective procedure to achieve this to maximize the successful treatment outcome. During disinfection, it is essential that optimal instrumentation be delivered, especially in the most apicalarea of the root canal. Studies on endodontic prognosis11 and root canal anatomy12-14 suggest that the optimal apical terminus is not the same for vital and non vital teeth. After single-tooth rubber dam isolation, the tooth and rubber dam were disinfected with 30% H2O2 and 5% tincture of iodine. 2% iodine potassium iodide was used for additional disinfection as a final rinse at each treatment session in cases of necrotic pulp with a periapical lesion. Before obturation, the canals were irrigated with 70% alcohol and completely dried. the risk of failure increases as the working length decreases (away from the radiographic apex). The interaction between the preoperative periapical diagnosis and the working length level was evaluated (Table VI). This model demonstrates a significant relationship between working length level and periapical diagnosis The effect of preoperative periapical diagnosis, working length, and density. Diseased periapex, decreasing working length (away from the radiographic apex), and fair/poor density all simultaneously increase the risk of failure For necrotic cases, it appears that success is enhanced when the root canal is instrumented and obturated closer to the radiographic apex, thereby effectively removing any remaining infected necrotic tissue. In contrast, for vital cases, the outcome is most favorable when the distance from the apex is maintained short of—but within 2 mm of—the radiographic apex.20 The question arises of whether instrumentation of necrotic cases with periapical disease carried out closer to the radiographic apex would have a success rate be similar to, for example, normal pulp/normal periapex or pulpitis/normal periapex. In other words, could we improve prognosis and equalize it for all diagnostic categories if we correctly determine the optimal level of instrumentation? A review of Table II reveals that the success rates for different diagnostic categories, taking into consideration the pulpal and periapical diagnosis, are significantly different. This table also clearly shows that the optimal level of instrumentation is different for different diagnostic categories. However, necrotic teeth with periapical destruction that are instrumented close to the apex still have a significantly lower success rate than the vital cases with normal periapex. This indicates that there are other forces at work in this complex system, independent of mechanical factors such as apical instrumentation terminus. These factors are most likely associated with the presence or absence of dentin infection and periapical complications.21 It is evident from the data presented in Table II that endodontic outcome is dependent on the preoperative periapical diagnosis, independent of the level of instrumentation or obturation. The favorable outcome, however, is enhanced by the differential level of instrumentation for different periapical conditions. From this we conclude that the endodontic outcome is primarily influenced by preoperative periapical diagnosis For roots/teeth with diseased periapex, the failure rate was approximately 20% higher if the density was fair/poor. In the series of the logistic regression models, we first analyzed the simultaneous effect of 2 factors: preoperative periapical diagnosis and working length level (Table V). This model shows that the risk of failure increases as the working length decreases (away from the radiographic apex). Compared with normal periapex, the risk of failure for teeth/roots with diseased periapex is higher for each level of working length (odds ratio _ 4.8; P _.0001). For every millimeter loss in working length (away from the radiographic apex), the odds of failure increase by approximately 14%. This model implies that, for normal periapex, the working length level does not matter and that any observed changes in failure risk are random. Furthermore, this model shows that the average rate of failure, across all levels of working length, is not affected by the working length in teeth/roots with normal periapex. A decrease in working length only increases the risk of failure in those with diseased periapex. Given the same periapical diagnosis and working length level, teeth/roots with a poor/fair density of obturation have more than double the risk of failure (odds ratio _ 2.18; P _.03). Regardless of density, a decrease in working length is associated with increased failure only in teeth/ roots with diseased periapex. It is now an established fact that teeth/roots with apical periodontitis have, on average, a rate of success that is 10% to 20% less than that in teeth/ roots without apical periodontitis.2,10 The prevalence of apical periodontitis increases with age, and by 50 years of age, 1 out of 2 individuals will have experienced this disease.23 The morbidity associated with apical periodontitis is higher in patients with certain systemic diseases than in the general population.24 Endodontic outcome is a multifactorial phenomenon. Internet Apexification is a method of inducing a calcified barrier at the apex of a nonvital tooth with incomplete root formation. Apexogenesis refers to a vital pulp therapy procedure performed to encourage physiological development and formation of the root end La povidone iodée est un complexe chimique soluble dans l'eau, composé d'iode et de polyvinylpyrrolidone (PVP). La povidone iodée est employée dans la pharmacopée comme antiseptique topique. Le médicament est commercialisé sous le nom Bétadine® en France, Belgique et Suisse. Le diiode I2 dissous dans l'éthanol (« teinture d'iode ») ou dans une solution aqueuse d'iodure de potassium KI (solution de lugol) est également utilisé en pharmacie et en milieu hospitalier comme antiseptique puissant. Il laisse des traces jaunes sombres caractéristiques sur la peau. Il existe également des composés organiques où l'iode est lié, tels que la povidone iodée (Bétadine ou IsoBétadine). La teinture d'iode est, en médecine, un antiseptique puissant composé d'iode dissous dans l’alcool, plus précisément dans l'éthanol, généralement à raison de 10 %. Il existe aussi des mélanges à 2 %, 3 % et 7 %. C'est un élément essentiel d'un kit de survie utilisé pour désinfecter et assainir. Cutting efficiency of 3 different instrument designs used in Reciprocation, Jeffrey Wan, BS,a Brian J. Rasimick, BS,b Barry L. Musikant, DMD,c and Allan S. Deutsch, DMD,c,d South Hackensack, NJ, and New York, NY With advancements in technology, endodontic instruments today come in a variety of designs, each differing in cost, performance, and safety. One important attribute is the cutting efficiency of an instrument. Cutting efficiency can be measured with several different techniques.1-4 One accepted method is to measure by weight the amount of dentin cut by the instrument under a reproducible procedure.5-7 An instrument’s flute design may be a determining factor of its cutting efficiency.8 Two major classes of endodontic instruments are reamers and files. Files are generally machined or twisted tightly to exhibit flutes that run more horizontal to the cutting surface, whereas reamers exhibit flutes directed more vertically. At the same cutting length, files have a greater number of flutes than reamers, which may have an effect on cutting efficiency. Other parameters have been observed by previous studies to affect cutting efficiency, including including surface treatment, cross-sectional area, sterilization, rake angle, tip design, and metallurgical properties.7-14 However, although extensive studies have been conducted on the cutting efficiency of rotary endodontic instruments, there is a need for studies investigating the cutting efficiency of reciprocating systems and flatsided instruments. All 3 instrument types were tested in reciprocal motion using an Endo-Express 4:1 reciprocating handpiece (Essential Dental Systems) operating at 2500 rpm. SafeSiders was found to cut significantly more than K-Files and K-Reamers (P _ .026), whereas K-Files and KReamers were not found to be significantly different (P _ .63). Studies observed a significant difference in cutting efficiency between machined and twisted instruments. 15,16 SafeSiders demonstrated a greater cutting efficiencythan either K-Files or K-Reamers. This may be attributable to the smaller cross-section of the SafeSider as a result of the integration of a flat side. According to Camps and Pertot,17 a smaller cross section creates more space between the instrument and the canal walls. This extra space allows for more debris collection, which facilitates easier removal. Larger cross sections may not provide enough space for debris to be displaced. As such, the debris impedes the instrument from cutting more dentin. The flat-sided feature may also increase cutting efficiency by introducing another cutting edge. However, this conclusion can neither be confirmed nor denied by this study. All instruments were operated under reciprocal cutting, whereby the cutting flutes oscillate back and forth about an axis that is parallel to the direction of cutting.Under this system, cutting efficiency improves as the angle between the cutting edge and the long axis of the instrument decreases.18 This would seem to favor reamers over files, as reamers exhibit flutes that are more vertical than those found in files. The results of this study do not support this supposition. Studies have reported smear layer thicknesses between 0.9 and 3.0 _m.23,24 The amount of dentin contained in the smear layer is less than 1.5% of the amount of dentin removed during instrumentation. The results of this study conclude that SafeSiders stainless steel 40/0.02 instruments exhibit a greater cutting efficiency on dentin than K-Files and K-Reamers of the same size and taper. K-Files and K-Reamers display a similar cutting efficiency. Cross-sectional area may be a determining factor on cutting efficiency. Pitch length does not seem to have an effect on cutting efficiency. Assessment of apically extruded debris produced by the singlefile ProTaper F2 technique under reciprocating movement Gustavo De-Deus, DDS, MS, PhD,a Maria Claudia Brandão, DDS, MS,b Bianca Barino, DDS, MS,b Karina Di Giorgi, DDS, MS,b Rivail Antonio Sergio Fidel, DDS, MS, PhD,c and Aderval Severino Luna, PhD,d Rio de Janeiro, Brazil No significant difference was found in the amount of the debris extruded between the conventional sequence of the ProTaper Universal NiTi files and the single-file ProTaper F2 technique (P _ .05). In contrast, the hand instrumentation group extruded significantly more debris than both NiTi groups (P _ .05). Conclusions. The present results yielded favorable input for the F2 single-file technique in terms of apically extruded debris, inasmuch as it is the most simple and cost-effective instrumentation approach. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;110:390-394) The use of the singlefile NiTi technique to prepare the whole root canal is very advantageous, because the learning curve can be considerably reduced with the reduction of the endodontic armamentarium. Moreover, the single-file NiTi technique tends to be more cost-effective than the conventional multifile NiTi rotary systems. The amount of material extruded from the apical foramen is one of the main concerns related to an instrumentation technique. Dentin debris, pulp tissue remnants, microorganisms, and intracanal irrigants may be extruded from the apical foramen during canal instrumentation. Extrusion ofthese elements may cause undesired consequences,such as induction of inflammation and postoperativepain and delay of periapical healing. 6,7 Although allinstrumentation techniques apically extrude someamount of debris, 8 there are notable differences among the techniques. It is worthwhile to note that,while apical extrusion of dentinal debris and irrigantshas been observed with the use of all presently known root canal preparation techniques and instruments, less dentinal debris extrusion was associated with the use of motor-driven rotary instruments.9,10 Because rotary instruments can differ greatly in their design, type of blades, use, number of files, and kinematics, different amounts of apically extruded debris can be found between the systems. 11 Conventional crowndown hand-file instrumentation was used as a reference. The null hypothesis tested was that there are no differences in the amount of debris extruded apically between the 2 ProTaper techniques. The apical third was prepared with Flexofile (Dentsply/Maillefer) sizes 50, 45, 40, 35, 30, and 25 at working length (WL) using the balanced force movement.14 Based on the statistical results, no significant difference was found in the amount of the debris extruded between the conventional sequence of the ProTaper Universal NiTi files and the single-file ProTaper F2 technique (P _ .05). The hand instrumentation group extruded significantly more debris than both of the other NiTi groups (P _ .05). The present study showed no significant difference in the amount of debris extruded between the conventional sequence of the ProTaper Universal NiTi files and the single-file ProTaper F2 technique. Therefore, the null hypothesis was plainly accepted. The improved apical control of debris extrusion promoted by both NiTi techniques is in line with earlier reports.9,10,16 Among several hand-instrumentation kinematics, the balanced force technique is regarded to promote less apical extrusion of debris.17 Therefore, the balanced force technique was chosen to be used as the reference for comparison in the present study In the present experimental design, 2 different variables were present in the NiTi groups: the number of files used and the movement kinematics. Therefore, it is not possible to isolate the influence of each variable on the present result. The experimental design used in this study is appropriate, because the purpose was not to determine the relationship between the number of files or the movement kinematics with the amount of debris extruded apically. However, it can be speculated that the canal preparation of singleroot teeth tends to extrude less debris, because the cleaning and shaping procedures are easier and more predictable. From a clinical point of view, the present results are favorable for the single-file F2 technique, inasmuch as it is the most simple and cost-effective instrumentation approach. However, apical control of extruded debris is just 1 aspect that an instrumentation technique needs to have tested. Other factors of the root canal preparation with the single-file F2 technique still require solid laboratory- based tests before the indication of large and longitudinal clinical studies; researching the involvement of the risk zone in the mesial root of mandibular molars, apical transportation, debridement ability, and fracture susceptibility all represent further priorities of research for the F2 single-file technique. The reciprocating movement is a clockwise (CW) and counterclockwise (CCW) movement. The ATR Vision (ATR; Pistoia, Italy) motor allows programming for reciprocating movement at four-tenths of a circle CW and two-tenths of a circle CCW. The F2 file was driven at 400 rpm with a 16:1 reduction ratio contraangle handpiece Lifespan of One Nickel-Titanium Rotary File with Reciprocating Motion in Curved Root Canals Sung-Yeop You, DDS, Kwang-Shik Bae, DDS, PhD, Seung-Ho Baek, DDS, PhD,Kee-Yeon Kum, DDS, PhD, Won-Jun Shon, DDS, PhD, and WooCheol Lee, DDS, PhD But : entre autre = to compare the timerequired for its preparation of a curved root canal using both reciprocating and continuous motion. Méthode = Each canal was prepared with reciprocating motion until the ProTaper F2 single file (Dentsply Maillefer, Ballaigues, Switzerland) reached the working length. One file was used until it was fractured. The access cavities were made with #330 burs, and the working lengths were determined as follows. A #10 K-file was inserted into the root canal until the tip of the file was flush with the apical foramen. From that point, 1 mm was subtracted, and that length was defined as the working length of the root canal. After the working length was determined, a glide path was produced using a #15 K-file. Reciprocating motion (clockwise 140_ and counterclockwise 45_) using the Tecnika digital motor (ATR, Pistoia, Italy) until a ProTaper F2 single file (Dentsply Maillefer, Ballaigues, Switzerland) reached the working length. One F2 file was used until it was fractured or deformed, after which it was replaced with a new file. All the root canals were prepared by one single operator. The fractured files were examined under the scanning electron microscope (SEM) (Hitachi S-4700; Hitachi, Tokyo, Japan) at magnifications of _30; _200; _1,000; _2,000; and _5,000 for disclosing the fracture mechanism. Another 60 canals from 30 of the extracted maxillary and mandibular molars were divided randomly into two groups: the continuous rotation motion (CM) group and the reciprocating motion (RM) group. The canals in the CM group (n = 30) were prepared using continuous rotation following the sequence of ProTaper S1->SX->S1->S2->F1- >F2, whereas the canals in the RM group (n = 30) were prepared with reciprocating motion with the sole use of the ProTaper F2. The average lifespan of one F2 file was 10.60 _ 4.35 canals with the longest lifespan of 21 canals. The total time for canal preparation was 46.42 _ 18.12 seconds and 21.15 _ 6.70 seconds in the CM and RM groups, respectively. There was a statistically significant difference between the groups (p < 0.01). Within the limitation of this study, one F2 file can be safely used to the working length of curved canals at least six times under reciprocating motion. Reciprocating preparation with only one F2 file was much faster than root canal instrumentation with continuous rotation. (J Endod 2010;36:1991–1994) The total root canal preparation time was measured until the F2 file finally reached the working length, excluding the time it took to change files and irrigate the canals. The average lifespan of one F2 file was 10.60_4.35 canals. The longest lifespan of a single F2 file was 21 canals, and the shortest one was 6 canals (Table 1). The total time for canal preparation in CM group was 46.42 _ 18.12 seconds, and the total time for canal preparation in RM group was 21.15 _ 6.70 seconds. There was a statistically significant difference between the two groups (p < 0.01). O ne of the goals of endodontic treatment is thorough cleaning and shaping of the root canal system by removing all the infected pulp tissue, bacteria, and their byproducts (1, 2). The NiTi rotary file has induced a significant progress in endodontic treatment because of its flexibility and high cutting efficiency (3–5). With the instrument placed in the center of the root canal, it has become possible to produce a more rounded and tapered funnel-shaped canal while reducing the incidence of procedural accidents such as transportation and ledge formation (6, 7). Furthermore, NiTi files prepare canals easily and rapidly with minimal straightening (8). Despite these advantages, the biggest problems of NiTi files are their high cost and unexpected instrument fracture (4, 9). The high cost of NiTi files has forced many clinicians to reuse them, which, in turn, leads to a higher incidence of instrument fracture. A recent retrospective study (10) showed that most file fractures occurred in curved root canals, especially in the mandibular molars. In this regard, the employment of reciprocating motion instead of the conventional continuous rotation method has been suggested to be advantageous in the preparation of curved canals with the use of one single NiTi file (14). However, it was a single case report of one operator, and there were no wellcontrolled comparisons with any existing methods. Reciprocating motion (clockwise 140_ and counterclockwise 45_) using the Tecnika digital motor (ATR, Pistoia, Italy) until a ProTaper F2 single file (Dentsply Maillefer, Ballaigues, Switzerland) When using a series of NiTi files, certain files bear more stress than others. For this reason, it is not possible to measure the lifespan of a certain file that reaches the full working length with completion of the root canal preparation. So far, apart from the recommendations to discard the NiTi file when it undergoes deformation or when the operator becomes instinctively insecure about the state of the file, there have been no reports on the fracture incidence of certain types of NiTi files. The data showed that the longest lifespan of single ProTaper F2 files with reciprocating motion was 21 canals with a mean lifespan of 10.60 canals. In this regard, Shen et al (16) reported on the mean lifespan of ProTaper files from two clinics. To our surprise, the result from one clinic showed that an average of 16.88 canals were prepared with one set of ProTaper files. However, it should be taken into consideration that the teeth included in their study were not limited to only molars. The data from other clinic in their same study revealed that only 2.83 molar cases were safely instrumented with sequential use of 6 ProTaper files. These results showed dramatic differences between uses in curved canals and in straight canals as well as the short lifespan of files in curved canals In this study, the minimum extent of ProTaper F2 usage before fracture was after six canal preparations, and this result seems to be consistent with Peters et al (17) who suggested that ProTaper files should be thrown away after being used in four to five constricted canals. According to another recent study (18), the lifespan of the ProTaper files was found to be 10 canals when they were used in sequence under continuous rotation, whereas these ProTaper files could be used in up to 13 canals without fracture when reciprocating motion was employed. Among the files used in their experiment, F2 files were shown to be reused in up to 10 canals without fracture under reciprocating motion, and this result was similar to our study in which the mean lifespan of the ProTaper F2 was 10.60 canals. When comparing the number of files needed in canal preparation, Varela-Patino et al (18) showed that a total of 23 ProTaper file series were needed to prepare 60 canals with reciprocating motion, and a total of 30 ProTaper file series were needed to prepare 60 canals under continuous rotation motion. In this study, only 11 F2 files were used to prepare 120 canals, and this was a big reduction in the number of files necessary to instrument to the working length. Furthermore, this is in contrast to one’s expectations that when using only one master apical size rotary file for the preparation of the entire root canal system, the stress exerted on this NiTI file should easily fracture it (19). These results can be explained by two reasons. First, the torsional stress was reduced by using reciprocating motion. Reciprocating motion prevents the taper lock phenomenon by unsymmetrical repeating of the clockwise and counterclockwise rotations (14, 18). As a result, it reduces torsional fracture, allowing us to understand how reciprocating motion shows better results compared with continuous rotation. The other reason is that the file used in this study was a ProTaper file. ProTaper files are known to resist torsional fracture by uniformly distributing the stress exerted on it (20). However, further research is needed to evaluate not only the stress distribution in other types of files under reciprocating motion but also the cleaning and shaping ability of a single file with reciprocating motion. Considering the efficiency of reciprocating motion, the total time taken for canal preparation in the RM group was half of that in the CM group. We noted a drastic reduction in the time needed for the preparation of curved canals to the working length with only one master apical size file under the reciprocating motion. This suggests that the reciprocating motion is superior not only in its mean lifespan but also in its cutting efficiency, which is ultimately expected to reduce the operator’s fatigue. Considering the efficiency of reciprocating motion, the total time taken for canal preparation in the RM group was half of that in the CM group. We noted a drastic reduction in the time needed for the preparation of curved canals to the working length with only one master apical size file under the reciprocating motion. This suggests that the reciprocating motion is superior not only in its mean lifespan but also in its cutting efficiency, which is ultimately expected to reduce the operator’s fatigue. According to a report by Sattapan et al (21), fracture of NiTi files occurs in one of two ways: flexural and torsional failure. Flexural fracture occurs because of repeated compression and tension in curved canal. Torsional fracture occurs when binding occurs at a part of the file other than the tip. In the clinical situation, both torsional stress and cyclic fatigue are exerted on files within the root canal, and these two forces influence each other. In fact, NiTi files exposed to torsional stress are prone to fracture at a lower cyclic fatigue (22), and torsional resistance decreases in used files (23). Therefore, these two aspects of file fracture can be simultaneously observed when the cross-sectional area of the fractured file was examined under the SEM (24). The problem in choosing NiTi files that have a high torsional resistance in most cases is that the cyclic fatigue value and the torsional resistance value are inversely proportional (4,25). In this regard, Ullmann and Peters (25) reported that larger instruments that have been subjected to some cyclic fatigue should be used with great care or discarded. The other method for preventing file fracture is to reduce torsional stress in the process of canal preparation. For this purpose, preflaring and the crown-down preparation have been suggested (9). These methods have been known to not only decrease the occurring torsional stress but also shifts the area on which the stress is exerted on (from the tip to the body of the file), further reducing any torsional stress. As it was reported by other studies (14, 18) and reconfirmed in this study, reciprocating motion reduces torsional stress by preventing binding of the file, thereby raising expectations that files could be used longer without fracture. In conclusion, within the limitation of this study, one F2 file can be safely used to the working length of curved canals at least 6 times under reciprocating motion without any help of other ProTaper files such as SX, S1, S2, and F1. Reciprocating preparation with only one F2 file was much faster than root canal instrumentation with continuous rotation. CLINICAL ARTICLE Canal preparation using only one Ni-Ti rotary instrument: preliminary observations G. Yared 102-83 Dawson Road, Guelph, ON N1H 1 B1, Canada Méthode = In this novel technique, the canal is negotiated to the working length with a size 08 hand file. Then, the canal preparation is completed with an F2 ProTaper instrument used in a reciprocating movement. In larger canals, the use of additional hand files may be required to complete the apical enlargement. The advantages of the technique include a reduced number of instruments, lower cost, a reduced instrument fatigue and the elimination of possible prion cross-contamination associated with the single use of endodontic instruments. Effective cleaning and shaping of the root canal system is essential for achieving the biological and mechanical objectives of root canal treatment. The objectives are to remove all the pulp tissue, bacteria and their by-products whilst providing adequate canal shape to fill the canal (Schilder 1974). However, nickel-titanium (Ni-Ti) instruments offer many advantages over conventional files. They are flexible (Walia et al. 1988), have increased cutting efficiency (Kazemi et al. 1996) and have improved time efficiency (Ferraz et al. 2001). Furthermore, Ni-Ti instruments maintain the original canal shape during preparation and have a reduced tendency to transport the apical foramen (Kuhn et al. 1997, Reddy & Hicks 1998, Ferraz et al. 2001, Pettiette et al. 2001). With all these apparent advantages, the use of Ni-Ti rotary systems has increased considerably since their introduction. However, their cost and instrument fracture (Alapati et al. 2003, 2004, Berutti et al. 2004) are notable disadvantages. The clinician is faced with two major concerns when considering the use of Ni-Ti rotary instruments: (i) possibility of instrument fracture associated with increased instrument fatigue caused by the repeated use and (ii) the possibility of cross-contamination associated with the inability to adequately clean and sterilize endodontic instruments (Spongiform Encephalopathy Advisory Committee 2006). A recent study found prions in human pulp tissue (Schneider et al. 2007). Tooth structure and organic debris were observed on the surface of Ni-Ti rotary instruments, and appeared to adhere in the surface cracks despite meticulous ultrasonic cleaning and decontamination (Alapati et al. 2003, 2004, Sonntag & Peters 2007). Therefore, the single use of endodontic instruments was recommended to reduce instrument fatigue and possible cross-contamination. However, the single use of endodontic instruments and, mainly the more expensive Ni-Ti rotary instruments, may become an economical burden on the endodontist and the general dentist especially as the available techniques involve the use of at least three to four Ni-Ti rotary instruments. Consequently, the introduction of canal preparation techniques which would reduce the number of instruments required to achieve the mechanical and biological objectives would be beneficial. This novel technique will reduce the number of Ni-Ti rotary instruments required for canal preparation, simplify the armamentarium and would be more cost-effective compared with other Ni-Ti rotary techniques with regards to the single use of Ni-Ti rotary instruments. F2 ProTaper Ni-Ti rotary instrument (Tulsa Dentsply, Tulsa, OK, USA). Instrumentation F2 An F2 ProTaper Ni-Ti rotary instrument is used for the canal preparation in a clockwise (CW) and counterclockwise (CCW) movement. The F2 is used in conjunction with a 16 : 1 reduction ratio contra-angle connected to an ATR Vision (ATR, Pistoia, Italy) motor which allows the reciprocating movement. The CW and the CCW rotations are set on the motor at four-tenth and two-tenth of a circle. The rotational speed is set at 400 rpm. The F2 instrument is used in the canal with a slow pecking motion and an extremely light apical pressure until resistance is encountered (i.e. until more pressure is needed to make the F2 advance further in the canal). The instrument is then pulled out of the canal, cleaned with a gauze to remove the debris filling the flutes, and reinserted and employed in the same manner. This step is repeated until the F2 reaches the working length. No further enlargement would be required for narrow and/or curved canals. For larger canals, hand files can be used after the F2 reaches the working length to complete the apical enlargement. The canal preparation is accomplished with continuous canal irrigation using a 2.5% solution of sodium hypochlorite and a final rinse with EDTA 17% followed by NaOCl. Voir la suite de la préparation dans ‘larticle Theoretically, the CW and CCW movement would reduce the incidence of torsional fracture by taper-lock. However, this issue needs to be investigated. One other important aspect of the F2 instrument is the variable taper. This feature would provide an increased flexibility for this larger instrument which can be used in this technique to prepare severely curved canals (Figs 1–3). An instrument with a fixed 0.08 mm mm)1 taper would be too rigid to be used in curved canals. The degrees of CW and CCW rotations were determined from the torsional fatigue profile of the F2 ProTaper instrument (unpublished results; Thompson 2006). These values were less than the degree of rotation at which the F2 instrument would fracture if bound in dentine (Thompson 2006). The CW rotation was greater than the CCW rotation. When the instrument is rotated CW, it will screw in the canal. When rotated CCW, the instrument will unscrew out of the canal. As the CW rotation is greater than the CCW rotation, the end result is a screwing in effect and an advancement of the instrument in the canal. Consequently, only very light apical pressure should be applied on the instrument as its advancement would be almost automatic At the present time, the ATR Vision motor (ATR, Pistoia, Italy) was the only one available in North America to allow the setting of the specific values of the CW and CCW rotations. In the standard Ni-Ti techniques using continuous rotation, it is crucial to enlarge the canal to at least a size 15 file prior to the use of rotary instruments (Peters et al. 2003, Berutti et al. 2004, Patino et al. 2005). If not, although the instrument would still advance in the canal, it would bind in dentine; instrument fracture may then occur due to taper-lock. In the present technique, the size 08 hand file ensures the presence of a patent glide path. The impression was that further enlargement with hand files may not be necessary even for the preparation of severely curved canals and Several authors (Stabholz et al. 1995, Ibarrola et al. 1999) recommended preflaring of the canal prior to its negotiation with hand files to the working length to minimize the incidence of procedural errors. In the present technique, the canal can be negotiated passively to resistance with a hand file size 8, and then the F2 is used to enlarge the canal to the length reached by the hand file. This step would be repeated until the hand file and the F2 reach the working length. Similar to the standard Ni-Ti rotary instrumentation techniques, one limitation for the application of the present technique is the presence of a sharp (non-gradual) canal curvature. In such a case, the instrumentation with the F2 would be carried to a level coronal to the curvature; the preparation of the apical part would then be completed with hand files. A novel canal preparation technique with only one Ni-Ti rotary instrument used in a clockwise and counterclockwise movement is described. This technique would offer two major advantages: (i) the single use of endodontic instruments would become more costeffective and (ii) the elimination of possible prion cross-contamination and a reduced instrument fatigue associated with the single use of endodontic hand and rotary reamers and files. Root Canal Preparation of Maxillary Molars With the Self-adjusting File: A Micro-computed Tomography Study Ove A. Peters, DMD, MS, PhD,* and Frank Paque´, Dr med dent† Introduction: The aim of this study was to describe the canal shaping properties of a novel nickel-titanium instrument, the self-adjusting file (SAF), in maxillary molars. Canals were shaped with the SAF, which was operated with continuous irrigation in a handpiece that provided an in-and-out vibrating movement. Changes in canal volumes, surface areas, and crosssectional geometry were compared with preoperative values. Canal transportation and the fraction of unprepared canal surface area were also determined. Results: Preoperatively, mean canal volumes were 2.88 _ 1.32, 1.50 _ 0.99, and 4.30 _ 1.89 mm3 for mesiobuccal (MB), distobuccal (DB), and palatal (P) canals, respectively; these values were statistically similar to earlier studies with the same protocol. Volumes and surface areas increased significantly in MB, DB, and P canals; mean canal transportation scores in the apical and middle root canal thirds ranged between 31 and 89 mm. Mean unprepared surfaces were 25.8% _ 12.4%, 22.1% _12.0%, and 25.2% _ 11.3% in MB, DB, and P canals, respectively (P > .05) when assessed at high resolution. Conclusions: By using SAF instruments in vitro, canals in maxillary molars were homogenously and circumferentially prepared with little canal transportation. (J Endod 2011;37:53–57) Cleaning and shaping of root canals successfully require the presence of irrigation solutions that can only be applied to the apical root canal third after enlargement with instruments (1–4). Nickeltitanium (NiTi) rotary instruments have become an important adjunct for root canal shaping, and outcomes with these instruments are fairly predictable (5). However, rotary instruments perform comparably poorly in long-oval canals such as distal canals in lower molars, specifically because they do not mechanically prepare 60% or more canal surface under these conditions (6). Very recently a new concept, the so-called self-adjusting file (SAF), has emerged that might allow uniform dentin removal along the perimeter of oval canals. Root canal preparation with this file has been quantitatively described only in anterior teeth (7) but not in molar root canals. The effects of root canal shaping were assessed, besides other approaches, from double-exposure radiographs (8), from cross sections by using the Bramante technique (9), and more recently by using micro–computed tomography (MCT) data (10). The latter technique allows nondestructive quantitative analyses of variables such as volume, surface areas, cross-sectional shape, taper, and the fraction of affected surface (11). Earlier studies had indicated that differences in canal anatomy between palatal (P), mesiobuccal (MB), and distobuccal (DB) canals would play a significant role for shaping outcomes (12). More ribbonshaped or flat canals such as the MB canal would have more unprepared canal area; moreover, on average, smaller more curved MB canals would have greater canal transportation than P canals. On the basis of the fact that the SAF is capable of addressing non-round canal cross sections, we hypothesized that various canals in maxillary molars can be prepared to similar outcomes with respect to canal transportation and amount of prepared surface. Studies based on MCT done in our laboratory during the last decade provided data on preparation effects for hand and rotary instruments in maxillary molars (10, 12–14). Therefore, the aim of this study was to describe the canal shaping properties of the SAF in maxillary molars. From teeth that had been extracted for reasons unrelated to the current study, 20 human maxillary molars were collected and stored in 0.1% thymol solution at 4_C until further use. Teeth had mature apices and were free of fractures and artificial alterations. They were mounted on scanning electron microscopy stubs and then scanned in a desktop MCT unit at an isotropic resolution of 20 mm(mCT 40; Scanco Medical, Bru¨ ttisellen, Switzerland) by using previously established methods (10, 15). Care was taken to specifically select teeth that did not have a distinct fourth canal orifice so as to include a buccolingually flat mesiobuccal canal, as judged from a preoperative MCT scan in low resolution. Teeth were then accessed by using high-speed diamond burs, and patency of the coronal canal was confirmed. Coronal flaring was accomplished with #2 Gates Glidden burs (Dentsply Maillefer, Ballaigues, Switzerland) placed to 2–3 mm below the cemento-enamel junction. Subsequently, canal lengths and patency were determined with size 10 Kfiles (Dentsply Maillefer) and radiographs; working lengths (WLs) were set 1 mm shorter than the radiographic apex. Each canal was then probed with #20 K-file. If it reached the WL, no further preparation was done. If the canal was narrower than that, it was prepared until #20 K-file could freely reach the WL to provide a glide path. A special irrigation device (VATEA; ReDent Nova) was connected to the irrigation hub on the file and provided flow of the irrigant (3% NaOCl) at a flow rate of 5 mL/min. Mean initial canal volumes in the apical 4 mm were 0.69, 0.31, and 0.91 mm 3 in MB, DB, and P canals, respectively (P < .01). Canal cross sections were rounder in DB and P canals compared with MB canals (P < .01, Table 1). Both preoperative volumes and SMI scores were statistically similar compared with samples of maxillary molars used in earlier studies (10, 12–14). Overall, canal preparation of root canals in maxillary molars with the SAF resulted in adequate canal shapes with no major shaping errors. In particular, no SAF fractured during the course of the study. On the basis of superimposed red-green coded surface areas (Fig. 1A), overall shapes were satisfactory, with similar amounts of dentin removed around the perimeter in most cross sections (Fig. 1A) and overall fully prepared canal surface areas. Preparing with the SAF for 4 minutes resulted in mean dentin removal ranging from 2.00–2.87 mm3; this represented significant volume changes compared with preoperative data (P < .01). Differences in volume increase were small but significantly different when comparing the 3 canal types investigated (Table 1). Increases in SMI were only significant for MB canals; 8 of 60 canals had SMI increases of 1 or more, all of which were MB canals. Slice-by-slice observation indicated that rounding of MB canals occurred mostly in the coronal third. Mechanically untreated canal areas, calculated by using superimposed MCT data sets (Fig. 1), were 25.8% _ 12.4%, 22.1% _ 12.0%,and 25.2% _ 11.3% for MB, DB, and P canals, respectively (Table 2); untreated canal areas were not statistically different when comparing the 3 canal types (P > .05). When restricted to the apical 4 mm, uninstrumented canal areas ranged from 28.8% in DB canals to 47.4% in P canals. When canal models were reformatted to 34-mm resolution, overall uninstrumented areas were 38.5% (Table 2). Mean canal transportation ranged from 31–149 mm and was larger in the coronal third compared with the apical and middle canal thirds (P < .01, Table 3). Canal transportation was lowest in the palatal canal. Differences between all canal types with respect to canal transportation at the middle and apical levels were significant; however, the individual canal transportation values exceeded 100 mm only in 15 of 120 cases at those 2 levels. No differences were registered when surface areas or canal transportation were recalculated on the basis of 34 mm compared with 20 mm. Dentin removal with the SAF is most effective during the first 2 minutes of use (18). However, additional time might be needed to ensure a full canal wall preparation in some cases; for example, 4 and possibly 5 minutes of activation were required in anterior teeth (7). Preparation with the SAF did not result in obvious preparation errors such as perforation and ledging, with canal transportation values typically below 100 mm for the middle and apical canal sections. The slightly larger canal transportation in the coronal section, particularly in MB canals, could have been possibly caused by Gates Glidden drills in an attempt to facilitate straight-line access. Overall, canal transportation is likely a cumulated effect of coronal flaring, glide path preparation, and the action of the SAF. Similarly, adding instruments for further apical enlargement tends to increase canal transportation, as shown in an MCT-based pilot study with sequential scanning (19). An earlier study with the same experimental setup had shown overall canal transportation scores of 123.7, 89.8, and 97.7 mm for the coronal, middle, and apical thirds, respectively, after preparation with NiTi rotary instruments or K-files (10). These scores and also those described for MB, DB, and P canals shaped with ProTaper (12) and FlexMaster (13) indicate larger canal transportation for rotary instruments than for the SAF in maxillary molar canals. In fact, our earlier study on the effect of SAF preparation on maxillary incisors (7) indicated that 5 minutes of shaping with the 2.0-mm SAF resulted in 91.4% treated surface, but only 56.6% surface had more than 100 mm dentin shaved off. The present study, on the basis of 20-mmresolution, demonstrates overall unaffected canal area of 25.2%. However, a recalculation to 34- mm resolution results in overall 38.5% unaffected area study on canals prepared to apical sizes #40 (MB, DB) and #45 (P) (10) indicated similar amounts of overall unprepared surface as in the present study (38.1%). However, rotary preparation of flat MB canals in maxillary molars in earlier studies (12, 13) resulted in 43.0% and 47.4% mean unaffected areas, respectively, which is higher than the scores in the present study. Taken together, cross sections from various slices (Fig. 1) and low scores for unaffected canal surface, in particular for flat canals, suggest that canal preparation with the SAF does indeed result in homogenous preparation and circumferential removal of a layer of hard tissue. In the present study, there were no significant differences in respect to affected canal surface among the canal types. Nevertheless, when the same SAF size is used (eg, 1.5 mm) for multiple canals in the same tooth, it might be prudent to increase preparation time for larger canal diameters. This will compensate for lesser forces of the cutting SAF elements against canal walls (18) in larger canals such as the palatal or the distal canal in molars. Alternatively, it might be advisable to instrument large canals before any smaller canals, on the basis of the tactile feedback during confirmation of the glide path. Rotary NiTi root canal files have been linked to 3%–5% incidence of intracanal breakage (23); although a retained instrument fragment per se might not significantly alter healing outcomes of periapical lesions, it is preferable to have no impediment to disinfection inside canals. In the present study we did not observe any SAF breakage with retained fragments Eradication of microorganisms, a critical step for endodontic outcomes (24), is the result of a combination of mechanical preparation (25) and irrigation (26). Irrigation alone is not always effective (27), and mechanical action of instruments on canal walls, including removal of infected dentin, might be needed. The preparation of the most apical canal section remains a challenge. In the present study, mechanical preparation with the SAF resulted in limited prepared surface. Hence, sufficient deposition of disinfecting irrigation solutions remains important. Antibacterial efficacy of canal surface preparation was not directly determined in the present study. Mechanical preparation per se might affect bacterial biofilms (28) rather than only microorganisms in their planktonic state. In fact, a recent scanning electron microscopic study suggested that preparation with the SAF leaves very clean dentin walls, probably as a result of concurrent irrigation possible with this system (6). the SAF and in particular its potential to debride canal walls better will lead to improved clinical outcomes, but clinical studies are underway to address this question. Another important clinical question is how best to obturate canals prepared with the SAF; initial data (29) suggest that lateral compaction resulted in better obturation quality after SAF preparation compared with rotary instrumentation. In conclusion, by using SAF instruments in vitro, canals in maxillary molars were homogenously and circumferentially prepared with little canal transportation or other procedural errors. Single-file F2 Protaper Technique in Oval-shaped Canals Gustavo DeDeus, DDS, MS, PhD,* Bianca Barino, DDS, MS,* Renata Quintella Zamolyi, MD, MS,† Erick Souza, DDS, MS, PhD,‡ Albino Fonseca, Ju´nior, MD, MS, Sandra Fidel, DDS, MS, PhD,§ and Rivail Antonio Sergio Fidel, DDS, MS, PhD§ Introduction: The aim of this study was to determine whether the debridement quality of the singlefile F2 Pro-Taper instrumentation technique is comparable to a full conventional ProTaper sequence in both round and oval-shaped root canals. The single-file F2 ProTaper technique displayed similar PRPT to the full range of ProTaper instruments in round canals. However, the debridement quality of the single-file F2 ProTaper technique was suboptimal in oval canals. Proper cleaning and shaping of the whole root canal space have been recognized as a real challenge, particularly in curved, narrow, or oval-shaped canals (1–6). By using the reliable micro-tomography method, Peters et al (6) reported that nickeltitanium (NiTi) instruments left about 35% of untouched root dentin surfaces. This deficient mechanical preparation could offer an opportunity for remaining microorganisms to recolonize the filled canal space, resulting in endodontic failure. In 2008, a new preparation technique using only the F2 ProTaper instrument in a reciprocating movement was published (7). The concept of using a single NiTi instrument to prepare the entire root canal is interesting, because the learning curve is considerably reduced as a result of technique simplification. Moreover, the use of a single NiTi instrument is more cost-effective than the conventional multi-file NiTi rotary systems. Although the first clinical impressions of the single-file F2 ProTaper technique appear promising (7), other important parameters still need to be properly assessed by both laboratory and clinical studies. Root canal debridement, for instance, is considered an important goal to be achieved after mechanical instrumentation of the root canal and is frequently measured in terms of cleaning efficacy (2–5, 8). In fact, the technique proposed by Yared (7) has never been assessed in terms of debridement quality. In the present study, we sought to evaluate the amount of residual pulp tissue after the use of either the full range of ProTaper instruments or the single-file F2 Pro-Taper technique in both round and oval canals. Two null hypotheses were tested: (1) there is no significant difference in debridement quality promoted by conventional full sequence of the ProTaper Universal NiTi files and the single-file F2 ProTaper technique in circular-shaped canals; (2) there is no significant difference in debridement quality promoted by conventional full sequence of the ProTaper Universal NiTi files and the single-file F2 ProTaper technique in oval-shaped canals With the purpose of collecting just vital mandibular incisor teeth, After each extraction, teeth were immediately placed into a vial containing 10 mL of buffered 10% formalin labelled with a random 4-digit alphanumeric code corresponding to 1 of the 4 experimental groups. For 6 months (August 2009–January 2010) following the abovementioned selection process, 98 mandibular incisor teeth were collected. Radiographs were taken in buccolingual and mesiodistal directions to select only teeth with a single root canal as well as to categorize them as oval or circularshaped canals. The space corresponding to the root canal lumen was measured 5 mm from the apex; when the mesiodistal diameter was 2.5 times larger than the buccolingual diameter, the canals were classified as oval-shaped; for round-shaped canals, the mesiodistal diameter had to be similar to the buccolingual diameter. All teeth presenting isthmus, lateral, or 2 canals had been eliminated. Furthermore, only root canals with an initial apical size equivalent to size 10 K-file were selected. As a result, just 54 incisor teeth fit the selection criteria. From the selected sample, 24 teeth were classified as circular-shaped and 24 as oval-shaped root canals. The 6 remaining teeth were used as histologic controls. A common silicone impression material was used to simulate the bony socket site. Tooth length was standardized to 18 mm, and the root canal patency was confirmed by inserting a size 10 instrument. The working length was established at the apical foramen. The use of different instrumentation techniques in both ovalshaped and circular-shaped root canals resulted in 4 experimental groups with 12 specimens each. Teeth for both experimental and control groups were randomly assigned with the aid of a computer algorithm (http://www.random.org). For both groups, 1 mL of 5.25% NaOCl solution was used between each instrument. To standardize the final irrigation volume used in the experimental groups, the volume of NaOCl was changed according to the number of instruments used in each technique. Thus, a total volume of 18 mL of NaOCl per treatment was used. The smear layer was removed with 3 mL of 17% ethylenediaminetetraacetic acid for 3 minutes. Three milliliters of bi-distilled water was used for 3 minutes as a final flush. Twenty-four teeth (12 circular-shaped and 12 oval-shaped canals) were prepared with the ProTaper Universal instruments (Dentsply Maillefer, Ballaigues, Switzerland) driven at 300 rpm and 2 N/cm of torque (XSmart; Dentsply-Maillefer) under rotary motion (group 1). The sequence was the following: (1) S1 file (1/3 of the working length [WL]); (2) SX file (1/2 of the WL); (3) S2 file (2/3 of the WL); and (4) F1 and (5) F2 files (full WL). Therefore, all the canals in this group were instrumented with 5 NiTi instruments. The other 24 teeth (12 circular-shaped and 12 oval-shaped canals) were prepared by the single-file F2 ProTaper technique (7); rotational speed was set at 400 rpm, and the F2 instrument was driven with ATR Teknica electric micromotor (Pistoia, Tuscany, Italy) under reciprocating movement (group 2). The specimens were visualized in Axioplan 2 Imaging fully motorized light microscope (Carl Zeiss Vision, Hallbergmoos, Germany). Image analysis and processing were completed by using the Axion Vision image 4.5 Zeiss system (Zeiss) to trace the outline of the area of interest. In this way, the crosssectional areas of each root canal and remaining pulp tissue were measured (mm2). Furthermore, the percentages of remaining pulp tissue area (PRPT) were calculated for each root canal section. Univariate analysis of variance demonstrated that both root canal shape and instrumentation technique significantly influenced the PRPT (P < .05). Much more tissue was removed in round canals than in oval ones in both techniques (P < .05). The shape of the canal significantly influenced the result of the technique, which can be seen by the significance in the interaction between canal shape and technique (P < .05). Group 1 removed significantly more pulp tissue than group 2 in oval canals (P < .05), whereas there was no difference between techniques in round canals (P > .05). The level of cross section did not influence the PRPT (P = .325). The descriptive data are displayed in Table 1. The present results show no differences in the debridement quality of circular-shaped canals prepared by the conventional full sequence of ProTaper Universal NiTi files and the single-file F2 ProTaper technique. Therefore, the first null hypothesis was accepted. On the other hand, the oval-shaped canals prepared by conventional ProTaper full sequence displayed significant improvement in debridement quality than those prepared with the single-file F2 ProTaper technique. Thus, the second null hypothesis tested was rejected. Recently, Taha et al (1) reported that the wide variation among different teeth prepared by the same technique appears to result from variations in root canal anatomy rather than from the instrument or technique itself. The results of the present study support this statement because the shape of the root canal significantly influenced the debridement quality. Thus, the complex anatomy of the root canal space must be restated as the critical challenge in infection control, especially considering the welldemonstrated limitations of the current root canal preparation techniques. The suboptimal results achieved by oval-shaped canals prepared by the single-file F2 ProTaper technique might have a 2-fold basis: (1) the reduced number of files and (2) the reciprocating movement kinetics. Because these 2 variables were present in this study, it is not possible to determine the influence of each one on the final result. Probably both variables are combining to result in the suboptimal performance by the single-file F2 ProTaper technique in oval canals. Infact, an additional appropriate experimental design is required to isolate the influence of the reciprocating movement on the debridement quality as well as the influence of the reduced number of instruments. The reduction in the number of instruments is a clear attempt to decrease the learning curve, also resulting in a cost-effective approach. However, the poorest debridement quality in oval-shaped canals can be considered as a side effect provoked by the simplistic approach of the single-file F2 ProTaper technique. The debridement quality of the root canal space has been reported to be more dependent on chemical than mechanical preparation once an adequate taper is obtained (9). However, single-file F2 ProTaper technique displayed significantly lower debridement ability in the present study, even with teeth presenting a similar taper preparation. This re-stresses the fact that even though the root canal space is radiographically properly enlarged, suitable debridement is not guaranteed. The poor debridement quality in oval-shaped canals reported here is not a novelty. In effect, ovalshaped canals have been representing a real challenge to clean, shape, and fill (9–12). By using the reliable micro–computed tomography method, Paque´ et al (12) observed more than half of unprepared dentinal walls (ranging from 59.6%–79.9%), regardless of the instrumentation technique used in oval-shaped root canals. The poor debridement quality found in the present study can be correlated to findings reported by Paque´ et al. The cross section images of the present study restate that rotary NiTi instruments tend to create round preparations in oval-shaped canals, leaving unprepared lingual and buccal extensions with remaining pulp tissue and the untouched dentin inner layer (1, 12) (Fig. 1B– F). This indicates that rotary NiTi systems are currently unable to produce optimal preparation of oval-shaped canals as a result of the large difference between instrument design and root canal geometry. In this context, entirely groundbreaking approaches for root canal instrumentation are required to optimize the debridement quality of oval-shaped canals. Therefore, innovative instruments such as the recently introduced self-adjusting file system (13–15) deserve indepth investigations. Remaining pulp tissue is the outcome measure used in the present experimental model to compare the cleaning efficacy of different shaping methods. The prospective in vivo selection of vital teeth by using a meticulous and well-controlled approach for diagnosing pulp vitality enabled effective standard of control for selecting teeth with tissue contents (16); which was confirmed by the positive histologic controls. This is an essential methodological step to assure the credibility for a comparative study with histologic assessment (16, 17). In summary, the present study demonstrated the influence of the root canal shape on the debridement quality, regardless of the preparation technique used. Single-file F2 ProTaper technique displayed the poorer debridement quality in oval-shaped canals. The present results lay emphasis on the debridement limitations of the current chemical-mechanical preparation methods as well as on the needed pursuit for more efficient instrumentation protocols. Root Canal Anatomy Preservation of WaveOne Reciprocating Files with or without Glide Pat Elio Berutti, MD, DDS,* Davide Salvatore Paolino, MS, PhD,† Giorgio Chiandussi, MS, PhD, Mario Alovisi, DDS,* Giuseppe Cantatore, MD, DDS,‡ Arnaldo Castellucci, MD, DDS, and Damiano Pasqualini, DDS* Introduction: This study evaluated the influence of glide path on canal curvature and axis modification after instrumentation with WaveOne Primary reciprocating files. Canal modifications seem to be significantly reduced when previous glide path is performed by using the new WaveOne nickel-titanium single-file system. N ickel-titanium (NiTi) rotary instruments were introduced to improve root canal preparation (1). In clinical practice these instruments are associated with an increased risk of fracture, mainly because of bending normal stresses (failure by fatigue) and torsional shear stresses (failure by torque) (2–4). Failure by torque might occur in case of torsional shear stresses exceeding the elastic limit of the alloy, producing plastic deformation and eventually fracture (4). The clinician and the type of instrumentation are fundamental to prevent torsional shear stresses. Various aspects might contribute to increase these stresses, such as excessive pressure on the handpiece (5), a wide area of contact between the canal walls and the cutting edge of the instrument (6, 7), or if the canal section is smaller than the dimension of the nonactive or noncutting tip of the instrument (6, 7); the latter case might lead to a taper lock, especially with regularly tapered instruments (8). The risk of taper lock might be reduced by performing coronal enlargement (9, 10) and creating a glide path before using NiTi rotary instrumentation (11, 12), both manual and mechanical (13). Thus, the root canal diameter should be bigger than or at least the same size as the tip of the first rotary instrument used (11–13). Canal curvature is considered one of the major risk factors for instrument failure caused by bending cyclic fatigue (1); stresses due to bending cannot be significantly influenced by the clinician No macroscopic deformations or fractures of any instrument occurred during the experiment. The new WaveOne NiTi primary reciprocating file, if used after a previous glide path, produced less modification in canal curvature compared with the Wave- One alone, as actually suggested by the clinical procedure flowchart (17). WaveOne NiTi files appear to maintain the original canal anatomy, and the presence of a glide path of the canal further improves their performance. Coronal enlargement (9) and preliminary creation of a glide path are fundamental for safer use of NiTi rotary instrumentation (11, 12). Furthermore, the preflaring of root canals would increase the accuracy of root canal length measurements with electronic apex locators (20). Reciprocating motion was proposed to increase canal centering ability as well as to reduce the risk of root canal deformity (21–23) the new M-Wire variant NiTi alloy instruments demonstrated better resistance on cyclic fatigue when compared with the same instrument design produced from stock 508 nitinol, preserving similar torsional properties (16). Canal scouting and preflaring are the first phases of canal instrumentation during which the clinician might more frequently find procedural difficulties (25). The use of a small-size hand K-file followed by a more flexible and less tapered NiTi rotary PathFile might be a less invasive and safer method to provide a glide path that better maintains the original canal anatomy, compared with manual preflaring performed with stainless steel K-file (13). Moreover, preflaring tends to minimize procedural errors such as transportation and ledge formation. Indeed, preflaring permits to maintain a pathway to the fullWL, avoiding excessive instrument binding in the canal (11, 12). In this study, it was observed that fewer pecking motions were needed to reach full WL with WaveOne single files, when previously glide path was performed. It might be hypothesized that this could reduce the risk of excessive undesired instrument brushing on the canal walls and subsequent root canal transportation (26–28). Furthermore, canal aberrations might lead to inadequate shaping and filling, affecting negatively the disinfection and the long-termprognosis of the root canal therapy (1, 29). Outer apical transportation and irregular foramen widening might lead to poor sealing efficiency with a high rate of extrusion of debris and postoperative discomfort (30–32). In this study the absence of a previous glide path affected the performance of WaveOne NiTi files, which evidenced greater alteration of the canal curvature, compared with the performance of WaveOne files with previous glide path. Effect of Canal Length and Curvature on Working Length Alteration with WaveOne Reciprocating Files Elio Berutti, MD, DDS,* Giorgio Chiandussi, MS, PhD,† Davide Salvatore Paolino, MS, PhD,† Nicola Scotti, DDS,* Giuseppe Cantatore, MD,‡ Arnaldo Castellucci, MD, DDS,§ and Damiano Pasqualini, DDS* This study evaluated the working length (WL) modification after instrumentation with WaveOne Primary (Dentsply Maillefer, Ballaigues, Switzerland) reciprocating files and the incidence of overinstrumentation in relation to the initial WL. A significant decrease in the canal length after instrumentation (95% confidence interval ranging from _0.34 mm to _0.26 mm) was detected. Conclusions: Checking the WL before preparation of the apical third of the root canal is recommended when using the new WaveOne NiTi single-file system. It has been shown that root canal instrumentation leads to changes in working length (WL) by straightening of the canal during the course of the treatment (17, 18). Although NiTi rotary instrumentation may lead to a smaller WL change during shaping procedures (19) compared with stainless-steel instrumentation, the appropriate determination of WL throughout the entire treatment is still a key factor for successful endodontic therapy. Davies et al (18) suggested that verifying the WL after early coronal flaring and late coronal flaring before definitive instrumentation of the apical segment of the root is the most appropriate action The present study evidenced that a significant decrease in the WL may occur after instrumentation with the new reciprocating single file WaveOne Primary, mainly because of the straightening of the root canal curves. These results are in agreement with the findings of other investigations on WL reduction after root canal shaping with stainless-steel hand files (21), stainless-steel hand files plus Gates Glidden burs (18), and NiTi rotary instruments (18, 22). However, it has been shown that NiTi rotary instrumentation may lead to a considerable, yet smaller, amount of curve straightening and to a WL decrease (18, 22). This is probably caused by the superior ability of NiTi instrumentation to remain centered into the root canal compared with stainless steel instrumentation (1, 18, 23). Modifications of the WL during instrumentation are influenced by several important factors. The original canal anatomy (ie, the canal curvature [17]) and the coronal flaring of root canals (24) were found to be the primary determining factors. In this study, the canal length and the canal curvature were correlated to the WL decrease. As expected, the interaction of both factors as well as the canal curvature by itself significantly influenced WL reduction; a severe curvature is more likely to lead to a higher decrease in the WL. On the contrary, straight canals, such as the ones used in the control group, did not undergo significant WL modifications after instrumentation. In case of severe curvature, flaring the coronal portion of the root canal should be the procedure of choice in order to facilitate the placement of files into the apical segment (24) and prevent excessive flexural stress to the Ni-Ti instruments (25, 26). It has been shown that, in case of severe curvature, the coronal flaring always leads to a WL decrease independently from the root canal preparation technique (27). However, WL reduction is higher after immediate coronal flaring compared with late coronal flaring before apical preparation (18). NiTi rotary files showed a mean WL decrease of_0.22 mmafter canal preparation, but a less pronounced WL reduction (_0.14 mm) was evident after late canal flaring. Therefore, it is highly recommended to check the WL at least twice during root canal shaping; an initial check is suggested during canal scouting. Canal scouting is usually performed with #10 K-file when the patency of the root canal is explored. Afterwards, a glide path is created, either manual or mechanical (11–13), to prevent NiTi rotary file tip blockage. A second check of the WL is suggested after canal flaring, before preparation of the apical portion of the root canal, because the WL is expected to undergo significant changes. This recommendation is particularly important when using the new WaveOne single-file reciprocating system, which is designed to reach complete shaping with only 1 instrument used to the full WL. In this study, the decrease of the canal length after instrumentation ranged from _0.34 mm to _0.26 mm (95% confidence interval). Furthermore, 24 of 32 WaveOne Primary files projected beyond the experimental apical foramen. The large incidence (75%) of overinstrumentation was simply caused by the fact that instrumentation was accomplished only according to the initial WL. Overinstrumentation with NiTi rotary files of augmented taper beyond the apical foramen may lead to apical transportation (28) and overfilling (29) with defective apical seal control, especially in severely curved canals. This type of intraoperative complication is a significant predictor of outcome of the initial endodontic treatment, especially in teeth with preoperative radiolucency (30). Furthermore, overinstrumentation may also cause complications such as a greater incidence of postoperative pain (31). The findings of this study confirm the importance of checking the WL when canal preparation has reached the limit between medium and apical third of the root canal before instrumentation of the apical third. Radiographic estimates have been shown to be inaccurate in determining the correct WL in many clinical situations, probably because of anatomic variability, and to be associated with the tendency to underestimation (32). Electronic apex locators showed to be precise and reliable in more than 90% of cases (33) and are superior in reducing overestimation of the root canal length (34). Their accuracy is improved by the flaring of the canal before WL determination (35). In conclusion, within the limits of this study, checking the WL before preparation of the apical third of the root canal is a highly recommended strategy when the new WaveOne NiTi single-file system is used. Cyclic fatigue of Reciproc and WaveOne reciprocating instruments G. Plotino, N. M. Grande, L. Testarelli & G. Gambarini Department of Endodontics, ‘Sapienza’ University of Rome, Rome, Italy To evaluate the cyclic fatigue resistance of Reciproc_ and WaveOne_ instruments in simulated root canals. Reciproc_ instruments were associated with a significantly higher cyclic fatigue resistance than WaveOne _ instruments. Reciproc_ and WaveOne_ instruments have been designed specifically for use in reciprocation. Both instruments have a lefthanded angulation of the blades, which means they cut in the counterclockwise (CCW) direction. The values of clockwise (CW) and CCW rotations are different. A large rotating angle in the cutting direction (CCW) determines the instrument advances in the canal and engages dentine to cut it, whereas a smaller angle in the opposite direction (CW) allows the file to be immediately disengaged and safely progress along the canal path, whilst reducing the effect of a screwing effect and file breakage. These angles are specific for the different instruments and they were determined using the torsional properties of the instruments. The instruments have the same nominal size, tip size 25 with 0.08 taper. Taper is constant in the apical 3 mm of the instruments but reduces in the middle and coronal portion of the working part of the instrument. 2006). The influence of the cross-sectional design of a NiTi instrument on its cyclic fatigue resistance is controversial and has been the subject of a number of recent investigations (Turpin et al. 2001, Biz & Figueiredo 2004, Diemer & Calas 2004, Chow et al. 2005). However, how and why the design of the instrument could influence their behaviour under cyclic fatigue stress remains unclear. In fact, some studies found that the fatigue life of various instruments did not seem to be affected by the instrument design, suggesting that the cross-sectional area or shape of the instrument is not the main determinant of fatigue life (Melo et al. 2002, Cheung & Darvell 2007). Yet, other studies on cyclic fatigue suggested that a different cross-sectional design appeared to be an important determinant of cyclic fatigue resistance of different files (Haikel et al. 1999, Grande et al. 2006, Tripi et al. 2006, Ray et al. 2007). However, in a previous study, Grande et al. (2006) demonstrated that the metal mass at the point of maximum stress influenced the lifespan of NiTi rotary instruments during a cyclic fatigue test. The authors compared the cyclic fatigue resistance of Mtwo_ instruments, with a lower cross-sectional metal mass, and ProTaper_ instruments, with a larger crosssectional metal mass, and reported that the bigger the metal mass, the lower the fatigue resistance. These results are consistent with those of the present study, which showed that Reciproc_ instruments, with a design similar to Mtwo_, are more resistant than WaveOne_ which has a cross sectional design similar to ProTaper_. It must be exp however, single use means that the same instrument can be used in 3–4 root canals, which could be complex and tortuous. Therefore, single use reduces but not eliminates the risk of accumulation of metal fatigue and failure. Hence, it may be concluded that testing cyclic fatigue of reciprocating instruments is as valuable as testing cyclic fatigue of rotary .instruments. Reciproc_ instruments resisted cyclic fatigue significantly more than WaveOne_ instruments; these differences could be related to the different cross-sectional design and/or the different reciprocating movement of the two instruments. Canal Shaping with WaveOne Primary Reciprocating Files and ProTaper System: A Comparative Study Elio Berutti, MD, DDS,* Giorgio Chiandussi, MS, PhD,† Davide Salvatore Paolino, MS, PhD, Nicola Scotti, DDS,* Giuseppe Cantatore, MD,‡ Arnaldo Castellucci, MD, DDS, and Damiano Pasqualini, DDS* This study compared the canal curvature and axis modification after instrumentation with Wave- One Primary reciprocating files (Dentsply Maillefer, Ballaigues, Switzerland) and nickel-titanium (NiTi) rotary ProTaper (Dentsply Maillefer). Canal modifications are reduced when the new WaveOne NiTi single-file system is used. (J Endod 2012;-:1–5 Furthermore, shaping tends to preserve the integrity and location of the canal and apical anatomy in preparation for an adequate filling (2, 5, 6). The avoidance of both iatrogenic damage to the root canal structure and further irritation of the periradicular tissue is demanding for all the newest instrumentation techniques (2, 7). Maintaining the original canal shape using a less invasive approach is associated with better endodontic outcomes (1). Previous studies have shown that canal transportation leads to inappropriate dentine removal, with a high risk of straightening the original canal curvature and forming ledges in the dentine wall (8, 9). Nickel titanium (NiTi) rotary instruments have shown efficiency in achieving optimal root canal shaping (1, 10), with less straightening and better centered preparations of curved root canals (2). The superelasticity of NiTi rotary files may allow less lateral forces to be exerted against the canal walls, especially in severely curved canals, reducing the risk of canal aberrations and better maintaining the original canal shape (1, 11) However, in clinical practice, these instruments may be subjected to fracture, mainly because of flexural (fatigue fracture) and torsional (shear failure) stresses (12–14). Torsional stresses may be increased with a wide area of contact between the canal walls and the cutting edge of the instrument (3, 15). To reduce such stresses, the ProTaper rotary design combines multiple progressive tapers, adequately maintaining the original canal curvature (1, 16, 17). Canal curvature is suspected to be the predominant risk factor for instrument failure because of flexural stresses and cyclic fatigue (1–3). The WaveOne Primary file has the same tip size and taper features as the ProTaper F2 but a variable section and reverse cutting blades. E NDODONTIC CANAL PREPARATION WAVEONE SINGLE-FILE TECHNIQUE by Clifford J. Ruddle,DDS The mechanical objectives for endodontic canal preparation were brilliantly outlined almost 40 years ago. 1 When properly performed, these mechanical objectives promote the biological objectives for shaping canals, 3D cleaning, and filling root canal systems (Figure 1).2 However, recent advances for endodontic canal preparation have focused on the concept “less is more.”3 This On the other hand, reciprocation, defined as any repetitive back-and-forth motion, has been clinically utilized to drive stainless steel files since 1958 Fortunately, these risks have been virtually eliminated due to continuous improvement in file designs, NiTi alloy, and emphasis on sequential glide path management (GPM).4 Compared to reciprocation, continuous rotation utilizing well-designed active NiTi files requires less inward pressure and improves hauling capacity augering debris out of a canal.5 Serendipitously, in about 1998, Dr. Ben Johnson and Professor Pierre Machtou co-discovered the unmistakable advantages of reciprocating NiTi files utilizing unequal bidirectional movements. Subsequently, in the late 1990s, Machtou and his endodontic residents extensively analyzed this novel unequal reciprocating movement using the entire series of not-yet-tomarket ProTaper files. Starting with the end in mind, Dr. Ghassan Yared, a former student of Professor Machtou, performed exhaustive work to identify the precise unequalCW/CCW angles that would enable a single reciprocating 25/08 ProTaper file to optimally shape virtually any canal. 7 Although this specific reciprocation technique stimulated considerable interest, this file was never designed to be used in this manner. Yet, Yared’s work rekindled interest to take this single-file concept closer to its full potential. WAVEONE In 2008, a team of 8 international clinicians including Drs. Ben Johnson, Sergio Kuttler, Pierre Machtou, Wilhelm Pertot, Julian Webber, John West, Ghassan Yared, and myself, in collaboration with Dentsply International, began the serious work of developing both a new reciprocating file and motor for shaping canals. In 2011, following 4 years of R&D, both WaveOne (Dentsply Tulsa Dental Specialties and Dentsply Maillefer) and Reciproc (VDW) were internationally launched as single-file shaping techniques. This paper will focus on WaveOne, as this single-file reciprocating shaping technique utilizing unequal CW/CCW angles is over 4 times safer and almost 3 timesis the system I helped develop and with which I am most familiar faster than using multiple rotary files to achieve the same final shape. 8,9 DESIGN Strategically, only 1 file is generally utilized to fully shape virtually any given canal. However, there are 3 WaveOne files available to effectively address a wide range of endodontic anatomy commonly encountered in everyday practice (Figure 2). The 3 WaveOne instruments are termed Small (yellow 21/06), Primary (red 25/08), and Large (black 40/08). The Small 21/06 file has a fixed taper of 6% over its active portion. The Primary 25/08 and the large 40/08 WaveOne files have fixed tapers of 8% from D1-D3, whereas from D4-D16, they have a unique progressively decreasing percentage tapered design. This design serves to improve flexibility and conserve remaining dentin in the coronal two-thirds of the finished preparation. Another unique design feature of the WaveOne files is they have a reverse helix and 2 distinct cross-sections along the length of their active portions (Figure 3). From D1-D8, the WaveOne files have a modified convex triangular cross-section, whereas from D9-D16, these files have a convex triangular cross-section. The design of the 2 WaveOne cross-sections is further enhanced by a changing pitch and helical angle along their active portions. The WaveOne files have noncutting modified guiding tips, which enable these files to safely progress through virtually any secured canal. Together, these design features enhance safety and efficiency when shaping canals that have a confirmed, smooth, and reproducible glide path. between martensite and austenite and produce a more clinically optimal metal than traditional NiTi itself. Studies have shown that M-wire technology significantly improves the resistance to cyclic fatigue by almost 400% compared to commercially available 25/04 NiTi files.10 The good news is that reducing cyclic fatigue serves to clinically decrease the potential for broken instruments There are 3 critical distinctions with this novel, unequal bidirectional movement. One, compared to continuous rotation, there is a significant improvement in safety, as the CCW engaging angle has been designed to be smaller than the elastic metallurgical limit of the file. Two, opposed to all other reciprocating systems that utilize equal bidirectional angles, the WaveOne system utilizes an engaging angle that is 5 times the disengaging angle. Fortuitously, after three engaging/disengaging cutting cycles, the WaveOne file will have rotated 360º, or turned one CCW circle. This unique reciprocating movement enables the file to more readily advance toward the desired working length.7 Three, compared to an equal bidirectional movement, an unequal bidirectional movement strategically enhances auguring debris out of the canal.11 Auguring debris in a coronal direction promotes the biological objectives for preparing canals, 3D disinfection, and filling root canal systems. From our collective experiences, our group can report that the primary 25/08 file will produce an optimal final shape in almost 90% of all canals, regardless of their length, diameter, and curvature. However, in longer, narrower, and more curved canals, even when the 10 file is loose at length, the Primary 25/08 WaveOne file will more predictably advance to the terminus of the canal when the glide path is expanded. The Large 40/08 WaveOne file is used to complete the shape in larger diameter canals that are typically straighter. Examples include certain maxillary incisors, single-canal bicuspids, and larger diameter canals within maxillary and mandibular molar teeth. Recall, the usual WaveOne protocol is to initiate shaping procedures using the primary 25/08 file. However, after carrying the Primary 25/08 file to the working length, gauging procedures may confirm that the foramen is bigger than 0.25 mm. In these instances, the clinician will require the 40/08 WaveOne file to fully shape and finish these larger canal systems. With experience, the clinician will learn to recognize these larger and more straightforward canals and is encouraged to initiate canal preparation procedures utilizing only the 40/08 WaveOne file. In summary, there are 3 WaveOne files. Following access and GPM procedures, the Primary 25/08 WaveOne file will generally progress to the desired working length in three or more passes. As previously mentioned, infrequently but on occasion, the clinician may require a second WaveOne file to complete a predictably successful final shape. SHAPING TECHNIQUE The WaveOne single-file shaping technique is beautifully safe and simplistic when attention in focused on the access preparation and GPM. As is required for any shaping technique, straightline access to each orifice is emphasized. Attention is directed to flaring, flattening, and finishing the internal axial walls.13 Importantly, the orifice(s) should be preenlarged and all internal triangles of dentin eliminated. Perhaps the greatest challenge performing endodontic treatment is to find, follow, and predictably secure any given canal At the end of the day, a small-sized hand file is used to either confirm existing space is available or, alternatively, to create sufficient space so mechanical files can safely follow a secured canal.13 To clarify, a canal is secured when it has a confirmed, smooth, and reproducible glide path. With an estimated working length and in the presence of a viscous chelator, insert a 10 file into the orifice and determine if the file will easily move toward the terminus of the canal. In shorter, wider, and straighter canals, a 10 file can usually be readily carried to the desired working length. A loose 10 file confirms sufficient existing space is available to immediately initiate mechanical shaping procedures utilizing the Primary 25/08 WaveOne file. However, in longer, narrower, and more curved canals, oftentimes the 10 file cannot be initially and safely worked to length. In these instances, there is generally no need to select and use size 06 and/or 08 hand files in an effort to immediately reach the terminus of the canal. Simply work the size 10 hand file, within any region of the canal, until it is completely loose (Figure 5). The advantages of a sequential glide path have been previously elucidated.4,1 When GPM procedures have been completed, the access cavity is voluminously flushed with a 6% solution of NaOCl. Shaping can commence, starting with the Primary 25/08 WaveOne file (Figure 6a). Gentle apically directed pressure will typically allow this instrument to run 2, 3, or 4 mm inward. After every few millimeters of advancement, or if the Primary 25/08 WaveOne file will not easily progress, remove this file and clean and inspect its flutes. Upon removing any mechanical shaping file from any canal, it is wise to irrigate, recapitulate with a 10 file, then re-irrigate. Strategically, recapitulating with the 10 file moves debris into solution, con- A brushing motion may be utilized to eliminate interferences, remove internal triangles of dentin, or to enhance shaping results in canals which exhibit an irregular cross-section. In one or more passes, continue with the Primary 25/08 file through the body of the canal. Removing canyons of restrictive dentin from the coronal twothirds of a canal creates a more direct path to its apical one-third, improving accuracy when determining a precise working length. Especially in longer, narrower, and more curved canals, the apical one-third of virtually any canal can be more predictably secured when pre-enlargement procedures have been performed first. A pre-enlarged canal improves the ability to more readily direct and slide a precurved small-sized hand file to the full working length. In the endodontic vernacular, “get behind the handle, gently slide the file, and find the foramen”. Regardless of the glide path sequence, once the apical one-third has been fully negotiated, establish working length, confirm patency, and verify there is a smooth reproducible glide path. The glide path is secured when a 10 file is loose at length and can “slip and slide” and “slide and glide” over the apical one-third of the canal.13 When the canal is secured, the Primary 25/08 WaveOne file can generally be carried to the full working length in one or more passes (Figure 7). When this Primary file will not readily advance in a secured canal, then the Small 21/06 WaveOne file may be utilized. This file will typically reach the desired working length in one or more passes. The Small 21/06 file may be the only shaping file taken to the full working length, especially in more apically or abruptly curved canals. However, with the anatomy in mind, to encourage 3D disinfection and filling root canal systems, more shape may be indicated. In these instances, the 25/08 file will generally advance through any region of a canal where the shape has been previously expanded utilizing the small 21/06 bridge file. Once the Primary 25/08 WaveOne file readily moves to the working length, it is removed. The finished shape is confirmed when the apical flutes of this file are loaded with dentin. Alternatively, the size of the foramen can be gauged with a size 25/02 hand file (Figure 8). When the size 25 hand file is snug at length, the shape is done. If the size 25/02 hand file is loose at length, it simply means the foramen is larger than 0.25 mm. In this instance, gauge the foramen with a size 30/02 hand file. If the size 30 hand file is snug at length, the shape is done. If the size 30 hand file is loose at length, proceed to the Large 40/08 WaveOne file to more optimally prepare and finish these larger canals. Upon reaching the working length, remove the 40/08 WaveOne file and inspect its apical cutting flutes. If the flutes are loaded with dentine mud, there is visual confirmation that this file has cut its shape in the apical one-third. Alternatively, the terminal size of the preparation can be gauged using a size 40/02 hand file. When the size 40/02 hand file is snug at length, the shape is done and the foramen is confirmed to be 0.40 mm. When the 40/02 hand file is loose at length, it simply means the foramen is larger than 0.40 mm. In these instances, other methods may be utilized to finish these larger, typically less curved, and more straightforward canals. There are differing opinions regarding the optimal size and taper to prepare the apical one-third of any given canal. Importantly, clinicians should not be trying to mechanically prepare “round” foramens; rather, clinicians should be trying to “clean” foramens. Cleaning is readily accomplished in a preparation that has a sufficiently tapered resistance form. Well-shaped canals promote the exchange of irrigants into all aspects of the root canal system.15 Evidence is available that clearly shows a 40/06 preparation is no cleaner than a 20/10 preparation.16 Articles have been accepted for publication that demonstrate the WaveOne shapes preserve and maximize remaining dentin, accurately follow and shape the original pathway of the canal, and maintain the position of the foramen (Figure 9).17 The WaveOne shapes of 25/08 and 40/08, in combination with active irrigation, have been shown to produce consistently clean root canal systems that any dentist can effectively fill in three dimensions (Figure 10).15 Zehnder : Root Canal Irrigants. JOE — Volume 32, Number 5, May 2006 pages 389-398 A favorable outcome of root canal treatment is defined as the reduction of a radiographic lesion and absence of clinical symptoms of the affected tooth after a minimal observation period of 1 yr (1). Evaluation of the Effect of Endodontic Irrigation Solutions on the Microhardness and the Roughness of Root Canal Dentin Hale Ari, DDS, PhD, Ali Erdemir, DDS, PhD, and Sema Belli, DDS, PhD Pecora et al. (23), Chng et al. (24), and Lewinstein et al. (7) reported that high concentration of hydrogen peroxide caused the great decrease in dentin microhardness. The significant alteration in dentin hardness after irrigation treatment indicates direct effects of these chemical solutions on the components of dentin structure (25, 26). Hydrogen peroxide affects the inorganic parts of dentin through acidic demineralization and attacks the organic-rich intertubular dentin by collagen denaturation action (7). Organic-dissolving properties of sodium hypochlorite on the collagen component of dentin (25, 26) explains how the alternated irrigation with these two solutions effect the hardness of dentin. Although 3% H2O2 had no effect on surface roughness of root canal dentin, a significant decrease on surface microhardness was shown in this study. This result probably may be related to the material’s low concentration. Zhender, 2005 Teeth were stored in 0.1% thymol solution at 5°C. Canals were instrumented using Gates Glidden drills followed by ProFile instruments (Dentsply Maillefer, Ballaigues, Switzerland) crown-down, so that finally a #45.04 instrument reached working length, which was set 1 mm short of the anatomical apex. During instrumentation, canals were irrigated with a 1% NaOCl solution. Subsequently, canals were rinsed with copious amounts of deionized water. To assess the impact of NaOCl on the complexing capacity of the sequestrant solutions under investigation, these were either mixed with H2O or 1% NaOCl at a 1:1 ratio. The instrumented teeth were randomly divided into 10 groups (n _ 6, each). In an experimental series, one tooth per group was irrigated for 1 min with 5 ml of the complexing agent/H2O or complexing agent/NaOCl mixture using an ISO-size 35 irrigating needle 1 mm short of working length (Hawe Neos, Bioggio, Switzerland). Distilled H2O and 0.5% NaOCl served as controls After experimental irrigation, teeth were copiously rinsed with deionized water and longitudinally fractured. After critical point drying, one fractured half of each root was mounted on a stub and goldsputtered (SCD 030, Balzers Union, Balzers, Liechtenstein). Prepared root canal surfaces were observed in a scanning electron microscope (AMRAY 1810, Bedford, MA), and five photomicrographs were taken at 500_ magnification from typical areas of the coronal, the middle, and the apical thirds of the prepared root canal. On each of these photomicrographs, the presence or absence of smear layer was semiquantitatively estimated by two blinded observers as follows: 0, 90 to 100% of dentinal tubules visible; 1, 50 to 90%; 2, 10 to 50%; 3, 1 to 10%; and 4, 0% of dentinal tubules visible. Median scores per tooth were used for further calculations and comparisons between different irrigation regimes. These were performed using Kruskal-Wallis ANOVA followed by Mann-Whitney U test. To assess the inter-dependence between calcium concentrations in eluates and smear layer scores in respective teeth, Spearman’s rank correlation coefficient was calculated. For statistical analyses, p _ 0.05 was considered to indicate a significant difference. Sectioning the Roots The anatomical crowns were removed with a separating disk. A shallow longitudinal groove was cut with a diamond separating disk on the buccal and lingual surfaces of the root. The roots were then split with a surgical chisel and mallet, resulting in a mesial and distal half for each canal. All intact halves were used for evaluation. Measurement of Surface Debris The root sections were mounted with the exposed canal system facing up on a calibrated grid. Photomicrograph ektachrome transparencies were taken of the specimens using a stereomicroscope at ×20 magnification. The transparencies were then coded and randomized by an investigator not associated with tooth instrumentation or the photomicrography. The photomicrographs were projected onto a screen to 24 times the original size to produce a 2 × 3 foot image. A transparent mylar grid was placed over the projected images. Each square in the grid represented a 200 ~m × 200 /,m subdivision of the root. An evaluator, who was unaware of which specimen or group was being scored, counted the total number of squares covering the apical 6 mm of canal space and the number of squares that contained debris (Fig. 1). Particles or chips of any structure on the surface of the root canal were judged as debris. A debris percentage was calculated for each specimen by dividing the number of debris containing squares by the total number of squares. A mean debris score was then calculated for each group. Differences among the treatment groups were determined using a one-way analysis of variance and Bonferonni post-hoc tests (p < 0.05).