Review
Lab Exercises 8-11
Exercise 8
What is the purpose of this exercise?
What is a capsule?
What is the capsule made of?
What is the importance of the capsule?
Describe the staining procedure for
staining the capsule
What bacteria we use for the capsule
stain procedure?
Exercise 8 cont.
What is a flagella?
What is taxis?
Why do we stain the flagella?
What bacteria we observed the flagella?
What is an endospore?
Why do bacteria produce spores?
Describe the composition of the
endospore
Exercise 8 cont.
When do endospores dissolve?
Describe the procedure to stain endospores
What bacteria we use for the endospore
stain procedure?
What is mycolic acid?
What antibiotics are used against TB?
Describe the procedure for acid fast stain
What bacterias we use for this staining
procedure?
Exercise 9
What is the purpose of the exercise?
Explain the major steps involved in obtaining
pure cultures from a mixed population
Explain the difference between selective and
differential media
Describe the appearance of the following
bacteria in EMB
E. coli, Enterobacter, Klebsiella, Proteus, Salmonella,
Shigella
Explain the color changes on EMB
Exercise 9 cont.
Describe the growth of the following bacterias
on TSA
M. luteus, S. epiderdimis, E. coli, E. aerogenes
Describe the growth of the following bacterias
on EMB
M. luteus, S. epiderdimis, E. coli, E. aerogenes
Describe the growth of the following bacterias
on CNA
M. luteus, S. epiderdimis, E. coli, E. aerogenes
Exercise 10
What is the purpose of this exercise?
Explain the composition of the blood
agar?
Differentiate between beta, alpha, and
gamma hemolysis on blood agar
Describe the composition and use of
Mannitol Salt Agar (MSA)
Explain the catalase test
Exercise 10 cont.
Explain the coagulase test
Explain the bacitracin or Taxo A disc test
Describe the composition and use of the
Bile Esculin Agar
Explain the 6.5% NaCl testing
Explain the Optochin or Taxo P disc test
Exercise 10 cont.
Describe the growth on blood Agar of:
S. aureus, S. epidermidis, E. faecalis, S. bovis
Describe the growth on MSA of:
St. aureus, S. epidermidis, E. coli
Describe the coagulase test on S. aureus
and S. epidermidis
Describe the growth of E. faecalis and S.
bovis on Bile Esculin Agar
Exercise 10 cont.
Describe the growth of S. bovis and E.
faecalis on 6.5% NaCl
Describe the growth of S. pyogenes with
Taxo A
Describe the growth of S. pneumoniae
with Taxo P
Exercise 11
What is the purpose of the exercise?
How do you differentiate Staphylococcus
and Streptococcus.
How do you know if you have S.
pyogenes in your throat?