Reproduction Ch.7 Asexual Reproduction • One parent • The prokaryotic chromosome is a ring of DNA • Binary fission-bacteria – Cell splits in two – Produces two genetically identical daughter cells, exactly like the original – Identical offspring (clones) • Different kinds – Binary fission - bacteria – Budding – yeast, sponges – Fragmentation – sea stars Binary Fission Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. chromosome 1. Attachment of chromosome to a special plasma membrane site indicates that this bacterium is about to divide. cell wall plasma membrane cytoplasm 2. The cell is preparing for binary fission by enlarging its cell wall, plasma membrane, and overall volume. 3. DNA replication has produced two identical chromosomes. Cell wall and plasma membrane begin to grow inward. 4. As the cell elongates, the chromosomes are pulled apart. Cytoplasm is being distributed evenly. 5. New cell wall and plasma membrane has divided the daughter cells. © Dennis Kunkel Microscopy, Inc./Visuals Unlimited SEM 2,345X Sexual Reproduction • Two parents • Sexual Reproduction – Cell splits twice – Produces four daughter cells, genetically different from the parent and each other – meiosis • Increases genetic variation Haploid vs. Diploid HAPLOID • 1n DIPLOID • 2n • One copy of each chromosome • Two copies of each chromosome • Homologous chromosomes-similar in size, shape and genetic content Homologous Chromosomes Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. •Homologous chromosomes-similar in size, shape and genetic content Sister chromatids a. duplication nonsister chromatids duplication kinetochore centromere chromosome homologous pair paternal chromosome b. chromosome maternal chromosome 6 a: © L. Willatt/Photo Researchers, Inc. How does Meiosis work? • Two stages, Meiosis I and Meiosis II • Meiosis I: Homologous chromosomes are separated from each other – Chromosomes replicate first, so each chromosome has a “twin” – Example: this is where the X and Y chromosomes separate from each other and go into different cells • X chromosome is a double X, Y chromosome is a double Y – Two new cells have 23 duplicated chromosomes • Crossing over-during Prophase I – Homologous chromosomes exchange genes while they’re lined up next to each other – You don’t inherit entire chromosomes from your parents—only pieces of them – Creates more variation and diversity within a species – Important for natural selection and evolution 2. Crossing Over • Homologous chromosomes swap genes • Happens during Prophase I • Increases genetic variation even more • Meiosis II – Just like mitosis, but with 23 chromosomes instead of 46 • Remember these are 23 duplicated chromosomes – Starts with 2 cells, which divide into 4 – In males: all 4 cells become sperm – In females: one cell becomes an egg, the other 3 die What’s a gamete? • Female gamete = egg (ovum); produced in ovaries; oogenesis • Male gamete = sperm; produced in testes; spermatogenesis Overview of Meiosis Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. • Diploid parent cell 2n = 4 14 Overview of Meiosis Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. centrioles nucleolus centromere chromosome duplication 2n = 4 2n = 4 MEIOSISI Homologous pairs synapse and then separate. 15 Overview of Meiosis Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. First division centrioles nucleolus centromere Second division Four haploid daughter cells sister chromatids synapsis chromosome duplication 2n = 4 2n = 4 MEIOSISI Homologous pairs synapse and then separate. n=2 n=2 MEIOSISII Sister chromatids separate, becoming daughter chromosomes. 16 Comparison of Mitosis & Meiosis Mitosis Results in 2 daughter cells Daughter cells are diploid Daughter cells are identical to each other and to parent Meiosis Results in 4 daughter cells Daughter cells are haploid Daughter cells are different from each other and from parent Mitosis v. Meiosis Mechanisms for Increasing Genetic Variation 1. Independent Assortment Each of the 23 pair of chromosomes separates into gametes independently (223different gametes can form=8 million possible gamete combinations) 2. Crossing Over • Homologous chromosomes swap genes • Happens during Prophase I • Increases genetic variation even more 3. Random Fertilization For humans - 23 pairs of chromosomes: Fertilization of an egg by a sperm is random # possible outcomes 223 (egg) X 223(sperm)= 64 trillion Determination of Sex •Females: two X chromosomes (XX) •Males: XY •All offspring will receive an X chromosome from the mother and either an X or a Y from the father •The X chromosome is large and contains over 1,000 genes •The Y chromosome is tiny and contains less than 200 genes