Treatments that stimulate the E.coli. to
take up foreign plasmids include:
0%
e
0%
ab
ov
nd
of
t
he
2a
1a
nd
I
3
0%
2
0%
Al
l
w
it h
sh
oc
k
0%
io
n
He
at
ba
t
In
cu
Ca
CL
2
tre
at
m
en
t
0%
EC
OR
1. CaCL2 treatment
2. Heat shock
3. Incubation with
ECORI
4. 1 and 2
5. 2 and 3
6. All of the above
If transformation with the Pglo plasmid is successful,
growth should occur on all plates except:
+DNA/lb/amp/ara
+DNA/lb/amp
-DNA/lb/amp
-DNA/lb
lb
0%
-D
NA
/
m
lb
/a
-D
NA
/
+D
NA
/
lb
/a
m
a
p/
ar
m
lb
/a
0%
p
0%
p
0%
+D
NA
/
1.
2.
3.
4.
In the green fluorescent colonies, what
is fluorescing?
The Pglo plasmid
The GFP
Arabinose
Uv light
Pg
l
ig
ht
0%
Uv
l
os
e
0%
bi
n
Th
e
GF
P
0%
Ar
a
o
pl
as
m
id
0%
Th
e
1.
2.
3.
4.
What is the puropose of the arabinose
in the Pglo transformation lab?
am
p
0%
nd
3a
re
sis
...
ge
ne
n
0%
4
0%
GF
P
th
e
n
It
t
ur
ns
o
li t
ur
ns
o
.co
It
t
eE
th
el
pd
ak
e
co
m
.. .
co
li.
E.
he
It
h
et
ak
It
m
0%
u.
..
0%
th
e
1. It make the E. coli.
competent
2. It helpd the E.coli
take up the plasmid
3. It turns on the GFP
gene
4. It turns on the amp
resistance gene
5. 3 and 4
In what region of the lambda virus
genome is gene H found?
At
ta
ch
m
en
t/
0%
is
on
ol
re
gi
n.
..
0%
lys
0%
Co
nt
r
Ta
il
in
te
gr
a
pr
ot
ei
n
s
te
in
pr
o
He
ad
0%
s
0%
tio
1. Head proteins
2. Tail proteins
3. Attachment/integrat
ion/excision
4. Control region
5. lysis
What is ECORI used for:
M
0%
0%
go
n
th
e
H
ge
ne
gD
NA
pe
te
nt
0%
Tu
rn
in
ak
in
gE
.co
li
co
m
M
ak
in
gg
el
s
0%
Di
ge
st
in
1. Making gels
2. Making E.coli
competent
3. Digesting DNA
4. Turning on the H
gene
A gene found on the PUC-18 plasmid
not found on the Pglo plasmid:
Bla
Lac Z
ECORI
2 and 3
0%
2a
nd
3
RI
0%
EC
O
Z
0%
La
c
a
0%
Bl
1.
2.
3.
4.
How many DNA fragments are produced why
lambda DNA is digested with ECORI?
0%
0%
7
0%
4
0%
2
0%
6
1
2
4
6
7
1
1.
2.
3.
4.
5.
After colonies grow on the Xgal/amp agar plate,
what do you do next with the colonies?
Tr
an
th
em
th
I
sh
oc
k
EC
OR
He
at
0%
0%
w
sfe
it h
rt
Ca
he
Cl
m
2
to
a
tu
be
. ..
0%
em
0%
Tr
ea
t
Tr
ea
t
th
em
w
it h
sfo
rm
th
em
0%
Tr
an
1. Transform them
2. Treat them with
ECORI
3. Heat shock them
4. Treat them with
CaCl2
5. Transfer them to a
tube of broth
Lysozyme is used in:
0%
ak
in
gr
Pl
a
0%
nt
PU
...
ec
om
sm
id
bi
na
is o
la
io
n
t io
n
0%
nf
or
m
at
Tr
as
ro
p
ho
re
sis
0%
M
Electrophoresis
Trasnformation
Plasmid isolation
Making
recombinant PUC18 plasmids
Ele
ct
1.
2.
3.
4.
What is agarose used for?
0%
ur
ni
n
go
n
th
e
H
ge
ne
DN
A
es
t in
g
li c
E.
co
in
g
ak
0%
4.
T
om
pe
t
in
g
M
ak
M
0%
en
t
ge
ls
0%
Di
g
1. Making gels
2. Making E.coli
competent
3. Digesting DNA
4. 4. Turning on the H
gene
What does electrophoresis sample
buffer contain?
ECO RI
CaCL2
Tracking dye
Glycerol
2 and 3
3 and 4
Tr
ac
3a
nd
4
0%
3
0%
nd
er
ol
0%
2a
kin
gd
ye
0%
Gl
yc
0%
Ca
CL
2
RI
0%
EC
O
1.
2.
3.
4.
5.
6.
The wells of the electrophoresis are
placed nearest:
1. The negative
terminal of the
chamber
2. The positive
terminal of the
chamber
0%
ve
po
sit
i
Th
e
Th
e
ne
ga
tiv
e
te
rm
te
rm
in
al
in
al
of
t..
of
...
0%
There are 4 DNA markers that we applied to our gel (784, 1120,
2010 and 3621). Which of these will migrate the furthest away
from the wells during electrophoresis?
784
1120
2010
3621
0%
36
21
0%
20
10
0%
11
20
0%
78
4
1.
2.
3.
4.
In which ECORI fragment of lambda
DNA do we expect to find the H gene?
3530
4878
5804
5643
7421
21226
0%
21
22
6
0%
74
21
0%
56
43
0%
58
04
0%
48
78
0%
35
30
1.
2.
3.
4.
5.
6.
Which colonies contain the plasmids that have a PUC18 plasmid with lambda DNA inserted into them ?
1. Blue colonies
2. White colonies
3. Green fluorescent
colonies
on
ie
s
0%
te
es
ce
nt
co
l
co
lo
ni
es
0%
Gr
ee
n
flu
or
W
hi
Bl
ue
co
l
on
ie
s
0%
In order to determine which lambda DNA fragment was
inserted into cells from white colonies, which
technique(s) did you do in lab?
n
RI
EC
O
0%
3a
nd
4
0%
re
c..
.
of
ho
re
sis
di
Ele
ct
is o
la
id
sm
Pl
a
0%
ge
st
io
...
an
tP
bi
n
m
Pr
ep
ar
e
re
co
0%
t io
n
0%
ro
p
1. Prepare recombinant
PUC-18 /lambda DNA
plasmids
2. Plasmid isolation
3. Electrophoresis
4. ECO RI digestion of
recombinant PUC-18
/lambda DNA
plasmids
5. 3 and 4
Which of the following is the lambda
genomic library?
hi
t..
.
w
of
a
be
p
tu
m
br
o
th
/a
Xg
al
0%
te
...
ag
ar
ro
p
el
ec
t
Th
e
in
ed
st
a
Th
e
0%
pl
a
an
.. .
m
bi
n
fr
ec
o
eo
tu
b
Th
e
0%
ho
re
s..
0%
Th
e
1. The tube of
recombinant PUC18/lambda DNA
plasmids
2. The stained
electrophoresis gel
3. The Xgal/amp agar plate
with white colonies
4. The broth tube of a
white colony from the
X-gal/amp agar plate.