MICROBIOLOGY BIOL260 Fall 2014 Instructors: Traci Kinkel, Ph.D. Suzanne Schlador Office Hours: Traci- by appointment Suzanne-MW 3:00-3:30pm and by appointment Course Hours: Saturdays 8:30-12:20pm and online Required Texts: Office: Traci AS1622 Suzanne IB2428A Office phone number: (206)-934-4579 (Suzanne) Email: Traci.kinkel@seattlecolleges.edu Suzanne.schlador@seattlecolleges.edu Microbiology, A Human Perspective, 7th ed., E. Nester, D. Anderson, C. Evans Roberts, and M. Nester, McGraw Hill Publishers, 2012. Microbiology Experiments: A Health Science Perspective, 7th ed., J. Kleyn, M. Bicknell, and A. Oller. McGraw Hill Publishers, 2012. Optional: A Photographic Atlas for the Microbiology Laboratory, 4th ed., Michael Leboffe and Burton Pierce. Morton Publishers 2011. Course Materials: We will be using Canvas to post lecture material, study guides, and other worksheets/activities for the course. Once you are registered for the course you will be able to access the course materials at this website: https://canvas.northseattle.edu. To log in to the course site for the first time: User ID = your full 9 digit Student ID # Password = the first six letters of your last name or your entire last name if it is fewer than six letters and repeat until you reach six letters Course Prerequisites: Two college level biology and chemistry lab courses or one of each with a grade of 2.0 or better. If you are completing this course as a pre-requisite for a nursing or allied health program, you should be taking this class after completing the human anatomy and physiology courses and all chemistry courses. This course transfers as upper division credits to the University of Washington. It is taught at a level that requires some prior familiarity with biology and organic chemistry. It will be difficult for you to do well if you do not already have an understanding of basic cell biology. Knowledge of basic chemistry is also necessary. Grading The final grade in the course will be based on the following items Lecture Exams Online Lecture Quizzes 40% 10% Lab Quizzes Lab Practical exam/Unknown Lab Lab Reports/Homework(Pre-class) Pre-labs 15% 20% 10% 5% General Course Layout and Logistics: This is a hybrid course, meaning that you will be expected to participate in both our online and in-person activities throughout the quarter. The website listed above will take you to the online course content. Note that parts of this website will ask you for a user name and password. Please notify me immediately if you have problems accessing any of the course content! In addition to the time spent thinking about microbiology on your own time and in our online community, we will also meet once each week on Saturdays to cover both lecture and lab content for the course. These in-person class sessions will consist of the following: 1. Solidifying our understanding of the week’s content through short lectures 2. Addressing any questions, open discussion on lecture/course content 3. Assessing your learning with lecture exams 4. Working in groups on in-class activities or lab exercises Please reference the lab schedule for a list of the labs we will be working on each week as well as when the lab quizzes and final lab exam (also known as the lab practicum) are scheduled. Lecture Exams: The exams may be composed of multiple choice, matching, and short answer questions. Please bring a Scantron and a #2 pencil for each exam. There will be no make-up exams unless special arrangements are made in advance. Online Lecture Quizzes: You are expected to complete an online quiz each week to help you assess your understanding of the week’s lecture material by 10 pm on Wednesdays. The quizzes will be openbook and timed, you will have 45 minutes for the quiz. On our course website you will find “reading guides” to help you focus on the key ideas from the chapters for the week. You should review these “reading guides” as well as read the material in the chapter before taking the online quiz. We cannot reopen a quiz for you, but will drop your lowest score for the quarter. Laboratory Practical Exam: The laboratory practical exam is a comprehensive exam based on experiments done in class. Anything done in the lab may show up on the exam. You may not use your notes or lab manual for the exam. It is a “practical” style exam which may include such things as identification of specimens under the microscope, identification of specific biochemical tests, recognition of bacteria on different types of agar medium. Unknown Lab: This final lab exercise is an opportunity to use your microbiology skills to identify the organisms present in a sample. Each student will work with their own sample and be responsible for identifying the genus and species of the organisms in the sample. Lab Reports: The lab reports will consist of the exercises found in the laboratory workbook and will include all data, drawings and completion of questions on the report pages of the exercise. They are due the lab period following completion of the experiment. Lab Quizzes: Quizzes are given the first 15 minutes of lab class and cover content from the lab exercises. There will be no make-up quizzes given due to tardiness or absence. Homework: The homework assignments for this course will consist of Pre-class assignments and other in-class activities. The focus of these assignments will be to clarify lecture concepts or apply lecture concepts to real-life situations. The pre-class assignments will be available through CONNECT and are designed to help you review the lecture material after reading the chapter. We expect that you will read the chapter(s) assigned for the week and complete the pre-class assignment found at our class site in Connect BEFORE class on Saturday. Pre-lab assignments: Each week you will be expected to complete pre-lab questions for each lab exercise assigned for the week. The pre-lab questions are found in the lab books at the beginning of each exercise. You will be expected to answer all the pre-lab questions and submit at the beginning of class on Saturday. Chemical Sensitivities: Due to the increasing numbers of individuals developing chemical sensitivities and the increasing awareness of such conditions, everyone who attends this class is asked to refrain from wearing any fragrance or perfume. The greatest feasible efforts will also be taken to ensure a fresh air environment free of not only the above mentioned fragrances but also potentially harmful substances such as carbon monoxide, formaldehyde, carpet odor, organic solvents, etc. Individuals who are unsure of the importance of this policy should see the Associate Dean for additional information. Student Services: Students with disabilities who believe that they may need accommodations in this class are encouraged to contact me as soon as possible to ensure that such accommodations are implemented in a timely fashion. Attendance and Commitment: Students should attend every class session. It is the student's responsibility to obtain lecture notes, handouts, or other materials in case of an absence. Since this is a specialized lab class requiring live organisms you will not be able to make-up any missed labs. A student who stops attending the class without an official withdrawal will be assigned a grade based on the work completed up to that point. This is a course that will require a great deal of individual effort by each student. You should expect to spend an average of 12-14 hours/week studying for this class. Electronic Devices: Out of respect for your instructor and fellow students, please turn off cell phones and pagers before class. No electronic devices may be used during exams. Your final grade will be based on the following NSCC scale: 4.0-3.5 A/A- 90-100% 3.4-2.9 B+/B 80-89.9% 2.8-2.2 2.1-1.5 B-/C+ C/C+ 70-79.9% 60-69.9% 1.4-1.0 D+/D 50-59.9% 0.9-0.0 D-/F Below 50% Academic dishonesty will not be tolerated, and will result in a ZERO for the affected exam, quiz, or assignment. A second offense will result in a withdrawal from the class for the remainder of the quarter. BIOL 260 LECTURE SCHEDULE DATE LECTURE TOPIC CHAPTER 9/27 (Sat) Online Quiz due Wed. 10pm Q1 Due Wed. 10/01 Microbial World 1 Cell Structure 3 __________________________________________________________________________________ 10/04 (Sat) Microbial Metabolism 6 Q2 Due Wed. 10/08 Microbial Growth 4 __________________________________________________________________________________ 10/11 (Sat) Food Microbiology 31 Q3 Due Wed. 10/15 __________________________________________________________________________________ 10/18 (Sat) Lecture Exam #1 Q4 Due Wed. 10/22 Antimicrobial Drugs 20 Microbial Control 5 __________________________________________________________________________________ 10/25 (Sat) Microbial Genetics 7, 8 Q4 Due Wed. 10/29 __________________________________________________________________________________ 11/01 (Sat) Viruses 13 Q5 Due Wed. 11/05 __________________________________________________________________________________ 11/08 (Sat) Genetic Transfer 8.6-8.9 Q6 Due Wed. 11/12 __________________________________________________________________________________ 11/15 (Sat) Lecture Exam #2 Q7 Due Wed. 11/19 Innate Immune Response 14 __________________________________________________________________________________ 11/22 (Sat) Adaptive Immune Response 15 Q8 Due Wed. 11/26 __________________________________________________________________________________ 11/29 (Sat) Host-Microbe Interaction 16 Q9 Due Wed. 12/03 __________________________________________________________________________________ 12/06 (Sat) Lab Practicum Exam Lecture Exam #3 __________________________________________________________________________________ BIOL260-MICROBIOLOGY LAB SCHEDULE Fall 2014 Sat. Class Date 9/27 10/04 Laboratory Exercise Ex. 3-Microscope Ex. 5-Simple Staining Ex. 1- Ubiquity of organisms Results-Ex. 1 Date 11/08 11/15 Laboratory Exercise Quiz #3 (Labs 12, 15, 14, 22) Results-Ex. 25, part 2 Ex. 23-Respiratory Organisms Ex. 24-results Results-Ex. 25, part 3 Results-Ex. 23 Ex. 6-Differential and Special Stains Ex. 2-Pure Culture Ex. 7-Definied, Undefined Ex. 22-Normal Skin Biota 10/11 10/18 Lab Quiz #1 (Labs 3, 5, 1, 6) Results-Ex. 2 Results-Ex. 7 Results-Ex. 22, part 2 Ex. 8-Quantitation Ex. 9-Aerobic/Anaerobic Ex. 12-UV lab Results-Ex. 8 Results-Ex. 9 Results- Ex. 22, part 3 Results-Ex. 12 Results-Ex. 22, part 3 11/22 Quiz #4 (Labs 17, 23, 24) Results-Ex. 25 Unknowns, part 4 Ex. 30-Epidemiololgy 11/29 Results-Ex. 30 Results-Ex. 25 12/06 Lab Practicum Exam Ex. 14-Antibiotics Ex. 15- Antiseptics/Disinfectants 10/25 Lab Quiz #2 (Labs 2, 7, 8, 9) Results-Ex. 14 Results-Ex. 15 Results-Ex. 22, part 4 Ex. 24-Gram negative rods 11/01 Results-Ex. 22 Results-Ex. 24 Ex. 17-Transformation Ex. 25-Unknowns, part 1