Cell Structure Chapter 6 IB Biology Prokaryotes vs. Eukaryotes • • • • • Prokaryotes: Prokaryotes have no nucleus or membrane bound organelles Most have a cell wall Many have flagella Very primitive Origin: 3.5 BYA Ex- Bacteria Eukaryotic Cells • “Eu” = true “Karyo” = kernal (nucleus) • “YOU are a Eukaryote” • Have a nucleus • Have membrane-bound organelles. • First appeared in fossil records 1.5 bya • All members of the following kingdoms have this type of cell: Protocista, Fungi, Plantae, Animalia. Prokaryotes/Eukaryotes** Prokaryotes Eukaryotes No nucleus Nucleus “Naked” DNA DNA associated with proteins DNA in cytoplasm DNA in an envelope (nucleus) No mitochondria Mitochondria 70s ribosomes 80s ribosomes No organelles organelles Reproduce by binary fission Reproduce by mitosis Ex.- Bacteria Ex.- all other cells Note: s = subunits Prokaryotic Cell Bacterium: Prokaryotic Cells Escherichia coli What do all cells have in common? •Cell membrane •Cytoplasm/cytosol •Genetic material •Ribosomes Cell Membrane • A selectively permeable phospholipid bilayer that forms a barrier between the cell and its environment Cytoplasm/cytosol •Liquid substance that all chemical reactions of life take place in Has dissolved ions, macromolecules • Genetic MaterialChromosomes • DNA is found as either organized chromosomes (eukaryotes) or single, loosely packed chromosome (prokaryotes) Ribosomes • Two-piece protein• based organelle that makes proteins in both major types of cell Varies structurally in prokaryotes (70S)and eukaryotes (80S). Generalized Animal Cell Generalized Plant Cell Differences between cells Plants Lack: Animals Lack: 1. Lysosomes 2. Centrioles 3. Flagella or Cilia 1. Chloroplasts 2. Vacuoles 3. Cell Walls 4. Plasmodesmata Eukaryotic Cell Organelles: Nucleus • Contains cell’s genetic • • • material in form of chromosomes Controls most of cell activities Site of transcription (1st step in protein synthesis) Has a double-membrane Eukaryotic Cell Organelles: Nucleolus • Smaller, dark body • inside nucleus Responsible for ribosome synthesis Eukaryotic Cell Organelles: ER • ER =Endoplasmic reticulum • Part of endomembrane • • system Smooth ER: no ribosomes, synthesizes lipids, detoxifies substances Rough ER: studded with ribosomes, transports proteins inside cell Rough ER makes membrane and proteins • The rough ER manufactures membranes • Ribosomes on its surface produce proteins Transport vesicle buds off 4 Ribosome Sugar chain 1 Polypeptide 3 Secretory (glyco-) protein inside transport vesicle Glycoprotein 2 ROUGH ER Eukaryotic Cell Organelles: Golgi Apparatus (not body) • Part of endomembrane system • The Golgi apparatus consists of • stacks of membranous sacs These receive and modify ER products, then send them on to other organelles or to the cell membrane Packages and exports cell chemicals such as proteins, carbohydrates and lipids Eukaryotic Cell Organelles: Mitochondria • Converts glucose into ATP during cell respiration • Is thought to have been primitive • • • prokaryote because: Has own genome (mtDNA) Directs own division Highly folded inner membrane Found in cells that are motile and contractible Has a double membrane, with inner membrane called the cristae. MITOCHONDRION Outer membrane Inner membrane Cristae Matrix Eukaryotic Cell Organelles: Chloroplast • Found in plant cells and some protists • Contains chlorophyll • Responsible for converting carbon dioxide and water into glucose during photosynthesis Eukaryotic Cell Organelles: The Cytoskeleton -Fibrous proteins (actin & tubulin) -Roles: Support, cell motility, biochemical regulation, organelle movement -Microtubules: •thickest (25 nm) •tubulin protein (hollow rods) •maintains shape, support, transport, chromosome separation -Microfilaments: •thinnest ( 7 nm) •actin protein filaments; •motility, cell division, shape -Intermediate filaments: • middle diameter (8-12 nm) •keratin; •shape, nucleus anchorage The Cytoskeleton Actin subunit Tubulin subunit Fibrous subunits 25 nm 7 nm MICROFILAMENT Figure 4.17B 10 nm INTERMEDIATE FILAMENT MICROTUBULE Basic jobs of each Microtubules Microfilaments Intermediate -give the cell rigidity -provide anchors for organelles -act as tracks for organelle movement -made of actin -enable cells to change shape and move -reinforce the cell -anchor certain organelles Eukaryotic Cell Organelles: Lysosome • Vesicle that comes off of Golgi body containing hydrolytic digestive enzymes Responsible for digestion of old, worn out cell parts and cells Also digests nutrients **Found extensively in Macrophages (WBC’s) • • Eukaryotic Cell Organelles: Centriole • Found only in animal cells • Composed of • microtubules in triplet arrangement Organizes microtubules that attach to chromosomes during cell division Eukaryotic Cell Organelles: Vacuole • Small ones in animal cells • Large ones found in plant • cells, stores water to maintain turgor pressure Can accumulate wastes, pigments in plant cells Eukaryotic Cell Organelles: Peroxisomes • Single membrane • Oxidative organelle ***strips e-’s (H’s) from substances Produce hydrogen peroxide (H2O2) in cells Metabolism of fatty acids; detoxification of alcohol (liver) Hydrogen peroxide then converted to water • • • Eukaryotic Cell Organelles: Cell Wall • Found only in plants • Made of cellulose • Non-living • Has small pores called plasmodesmata, which allows materials in and out. Eukaryotic Cell Organelles: Cilia/Flagella • Cilia: • short hairlike projections used in cellular movement Flagella: whiplike projection used in cellular movement Structure in prokaryotes is slightly different ECM: Extracellular Matrix** • Extracellular matrix (ECM) composed of (located on outside of cell): -Proteins & Carbodydrate -Specifically: -glycoproteins -glycolipids -integrins -fibronectins -collagen (50% of all protein in the body) Extracellular Matrix (ECM) - Pg. 118-120 Animal cells secrete or produce: Glycoproteins: •proteins covalently bonded to carbohydrate Collagen (50% of protein in human body •embedded in proteoglycan (another glycoprotein-95% carbohydrate) Fibronectins bind to receptor proteins in plasma membrane called integrins (cell communication?) Functions: support, adhesion, movement** ECM’s in Plants** Cell Walls are an ECM. -maintain shape -prevents excessive water uptake, and -holds the plant up against the force of gravity. Samples of Various Types of Cells • Protists may have contractile vacuoles – These pump out excess water Nucleus Contractile vacuoles Figure 4.13B • Cell, stained for mitochondria, actin, and nucleus Figure 4.1x • Prokaryotic cells, Bacillus polymyxa Figure 4.4x1 • Prokaryotic cell, E. coli Figure 4.4x2 • Pili on a prokaryotic cell Figure 4.4x3 • Prokaryotic flagella Figure 4.4x4 • Prokaryotic and eukaryotic cells compared Figure 4.4x5 • Paramecium, an animal cell Figure 4.5Ax • Plant cells Figure 4.5Bx1 • Chloroplasts in plant cells Figure 4.5Bx2 • Nuclei (yellow) and actin (red) Figure 4.6x Red Blood Cells