Advanced molecular genetics
224
Instructor
e.Mail me to set an appointment
Nohakhalifa@hotmail.com
Office, Botany Department
Room #239 in the first floor
Course contents
The history of DNA discoveryIntroduction to classic genetics Introduction to molecular genetics - DNA structure and function
- What is the gene?
- Replication, transcription and translation
processes in prokaryotes and eukaryotes.
- RNA and protein
- Gene expression
- Molecular genetic techniques
-Genetic engineering and DNA cloning
Course requirements
Weekly in class quizzes
Take home assignments ( a assignemnts)
Midterm exam ( for lecture and lab)
Final exam ( for lecture and lab)
Oral presentation
Attendance
Lab session
-Biostatistics
Data presentation in your lab report( making graphs and tables)-
Lab session
-DNA
Isolate DNA and determine its concentration.
- Analysis of DNA using gel electrophoresis.
- DNA amplification using PCR techniques
- DNA sequencing ( classic and modern methods)
-Restriction enzymes and restriction map.
Lab session
Protein
- Isolation and analysis
- Electrophoresis and concentration
determination
- Western technique
Lab session
Genetic engineering
- DNA cloning and plasmid design
- Transformation and competent cell
preparation
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Lab session
-Oral presentations
Introduction of some concepts
Micro and macro molecules
Micro-molecules:
Glucose, amino acids ….etc.
Macro-molecules
DNA, RNA and proteins
Isolation of macromolecules
Break the cell open
This process is called
cell lysis
Cells must be placed
in a buffer solution
during the lysis
process. why?
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Isolation of macromolecules
Hierarchy & Protein Function
Protein Folding II
Regulation of Protein Function by
Degradation
electrophoresis
SDS-PAGE
Introduction to genetics
What is the genetic material
S and R bacterial cells
Streprococcus pneumonia
Hershy –Chase “blender”
experiment using T2
bacteriophage
Bacteriophage
How genes are transmitted from
parents to offspring?
Classical or mendelian genetics
Gregory Mendel experiment on Garden
peas 1856-1863
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Classical genetics depends on:
Phenotype: 
color , size , texture…etc.
Genotype: 
The genetic make up of the
organism.
Inheritance of seed texture
Morphological phenotype
W is dominant over the w
Inheritance of seed texture
Molecular phenotype
Mendel first experiment
Inheritance of Genetic trait(s)
Mendel first experiment
reciprocal cross
Results of Mendel first experiment
Explanation of 3 dominant :1
recessive inheritance
Monohybrid cross
Independent assortment
Segregation
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The principal of the
3:1 inheritance
Phenotype:
Genotype: