Microinjections:
① Injection lines for site-specific integration:
y sc v nanos-integrase; attP2 (the integrate site is on the 3rd chromosome)
y sc v nanos-integrase; attP40 (the integrate site is on the 2nd chromosome)
② If you provide the DNA (20ug), please use Qiagen Mini Prep Kit to extract plasmid.
③ Vectors for site-specific integration:
Vector name
Integrate site
Cell type
Reference
VALIUM1
attp2,on 3rd
chromosome
Somatic cells
Ni et al., 2008, Nature Methods
VALIUM10
attp2,on 3rd
chromosome
Somatic cells
Ni et al., 2009, genetics.
VALIUM20
attp2,on 3rd
chromosome,or
attp40,on 2nd
chromosome
Somatic and female
germ line cells
Ni et al., 2011, Nature Methods
VALIUM22
attp2,on 3rd
chromosome,or
attp40,on 2nd
chromosome
Mainly for Female
germ line cells
Ni et al., 2011, Nature Methods
These vectors can be used for protein overexpression as well.
For p-element vector injection, please contact Luping Liu,
email: lpliu@mail.tsinghua.edu.cn
Tell: +86-10-62794230
④ Cost:
If you provide the DNA for injection, you will receive the transgenic fly about six weeks later, we
charge 1500￥($240) per transgenetic line.