Developing a dye using
Caesalpinia sappan (Pathangi)
wood extract
BT 3064 (Experimental Plant Biotechnology)
Department of Plant Sciences
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BT 3064 (Experimental Plant Biotechnology)
course module
Level III Plant Biotechnology (Special Degree)
Group work of 6 students
• K.P.D. Tharaka
• W.A.K.T.M. Anandawansha
• P.N.A. Dayananda
• P.V.D. Dilani
• U.W.R. Sampath
• D.I.M. Yahampath
Supervised by,
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Dr. Sudeera Ranwala
Dr. Pradeepika Saputhanthri
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What are natural dyes
Dyes derived from natural sources
Dyes are coloured, ionizing and aromatic
organic compounds which show an
affinity towards the substrate to which it
is being applied
( Nemcova et al, 2014)
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Why use natural dyes?
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Provide an environment friendly dye for
fabrics and garments
Do not contain chemicals harmful to health
and more reliable than the chemical
synthetic dyes
Reducing production costs and eliminating
the huge expenses of chemical imports
Biodegradable and do not cause disposal
problems
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Pathangi (Caesalpinia sappan) as a
natural dye
• The heartwood of the plant is used as
dyeing agent
• The main dye component in the heartwood
is brazilin
• Brazilin is a weakly coloured
product
• Easily oxidises by air and light
to give the deep red pigment
brazilein.
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Our tasks : Aims and objectives
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To improve the extraction protocol by testing
the effectiveness of different solvents.
To investigate the effect of incorporating
Kora-Kaha (Memecyclon umbellatum ) leaves
in the extraction procedure.
To find out most appropriate dye amount and
mordanting method for effective dyeing of
fabrics.
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Our tasks : Aims and objectives cont..
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To check the effectiveness of the dye, after
application through certain techniques such
as wash fastness, rub fastness, and photo
degradation effect.
To check for microbial contaminations after
application of dye to a cotton fabric.
To develop Pathangi crude as a paint for
cellulose papers and to obtain several
different colours using different mordants.
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Each student conducted standard
methodologies
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Student 1- Extraction of the dye from the wood of
Pathangi using different extraction
methods
Student 2- Investigate the effect of incorporating
Kora-Kaha leaves in the extraction
procedure
Student 3- Determination of photo degradation
effect
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Student 4- Mordanting of clothes, dyeing of
mordant clothes and solubility tests for
the crude extracts
Student 5- Determine the growth of microbes after
been applied to clothes
Student 6- Development of the crude extract
(concentrated) as paint
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Results
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Extraction of the dye using different solvents.
- Best solvent- methanol
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Preparation of the crude of Pathangi
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Determination of photo degradation.
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Liquid extractions
After applied to clothes
Colour degradation
highest- water extraction
Lowest- methanol extraction
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Absorbance
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water
methanol
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0
200
300
400
500
600
700
wavelength (nm)
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Mordanting of clothes
Best mordant- a mixture of
alum (Potassium Aluminium Sulphate) &
cream of tartar (Potassium hydrogen tartrate)
Colour fainting has been minimized using mordants
Non mordent clothes
Mordent clothes
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Discolouration and visible signs of microbial
growth
No colonies or discolouration patches on dyed
clothes
Development of the Pathangi crude as paint.
Different colours were obtained using different
mordants
Mordant used
Colour of the crude.
Alum
Maroon/deep red
CuSO4
Dark Brown
FeSO4
Brown
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What we have gained..??
Group Work
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How to work as a team
How to face challenges
Work within given time duration
How to deal with others
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Acknowledgement
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Our supervisors, Dr.S.Ranwala and Dr.P.S.Saputhanthri,
senior lecturers, Department of Plant Sciences
All other lecturers, demonstrators & non academic
staff of Department of Plant Sciences
Biodiversity secretariat - Wickramasinghepura, Athul
Kotte
Our dear parents
All of our colleagues
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Thank You
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