Units of Concentration A solution is a homogeneous mixture of one substance (the solute) dissolved in another substance (the solvent). Concentration is a ratio of the amount of solute to the amount of solvent. Units of Concentration Percent volume % volume = volume solute (ml) volume solution (ml) x 100 Percent mass % mass = mass solute (g) mass solution (g) x 100 Solution = solvent + solute Units of Concentration Example 1: What is the percent by volume concentration of a solution in which 75.0 ml of ethanol is diluted to a volume of 250.0 ml? 75.0 ml x 100 250.0 ml = 30.0% Units of Concentration Example 2: What volume of acetic acid is present in a bottle containing 350.0 ml of a solution which measures 5.00% concentration? x 350.0 ml = 0.05 x = 17.5 ml Units of Concentration Example 3: Find the percent by mass in which 41.0 g of NaCl is dissolved in 331 grams of water. 41 g 372 g x 100 = 11.0% Units of Concentration Molarity (M) is the most common unit of concentration Molarity is an expression of moles/Liter of the solute. Units of Concentration A mole is the SI unit of number of particles and can be used as an expression of the molecular weight of a substance. The formula weight of an element is expressed as grams/mole Units of Concentration The molar mass of a compound can be calculated by adding the molar mass of the individual elements. 22.99 + 35.45 = 58.44 g/mol Making Solutions You just calculated the molar mass of sodium chloride to be 58.44 g/mol. To determine how to make a stock solution of sodium chloride, use the formula: g = M x L x molar mass Making Solutions How many grams of NaCl would you need to prepare 200.0 mL of a 5 M solution? g = M x L x molar mass g = (5mol/L) (0.2L) (58.44g/mol) g = 58.44 g Diluting Solutions Often once you have made a stock solution, you need to dilute it to a working concentration. To determine how to dilute the stock solution, use the formula: C1V1 = C2V2 C1 C2 V1 V2 – concentration of stock - concentration of diluted solution – volume needed of stock – final volume of dilution Diluting Solutions Example 5: How many milliliters of a 5 M stock solution of NaCl are needed to prepare 100 ml of a 0.4 M solution? C1 V1 = C2 V2 (5) V1 = (0.4)(100) V1= 8 ml Diluting Solutions Serial Dilutions are dilutions made in series (for example, if you needed to make solutions that were 2M, 1M, 0.5M, and 0.25 M) The formula for serial dilutions is: Dilution Factor = (V1 + V2) V1 V1 – volume of solution being diluted V2 – volume of solvent Units of Concentration Example 6: Propose a method to prepare 100 ml of a 0.5 M glucose solution from a 5 M glucose solution. 10 = (v1 + 100) v1 10v1 = v1 + 100 -v1 -v1 9v1 = 100 9 9 v1 = 11.1 ml of 5 M glucose + 100 ml H2O Diluting Solutions Dilutions tutorial