Biotechnology Biotech • Use of living systems and organisms to develop or make useful products Biotech • Determining sex • Forensic/identity testing • Prenatal diagnostic screening • Risk of developing adultonset cancers • Predicting adult-onset disorders. Biotech • Today in our lab, biotech in action…………….. Blood Typing • A blood type (also called a blood group) is a classification of blood based on the presence or absence of antigenic substances on the surface of red blood cells (RBCs) Blood Typing • An antigen is a substance or molecule that, when introduced into the body, triggers the production of an antibody by the immune system. Blood typing • Antibody: a protein used by the immune system to identify and neutralize foreign objects like bacteria and viruses • Each antibody recognizes a specific antigen unique to its target Blood Typing • antigens are bacteria or virus that can cause an illness • antibodies are part of the immune system and identify and fight against the foreign bacteria Blood Typing • There are two different types of antigen – type "A" – type "B“ • The ABO blood type classification system uses the presence or absence of these molecules to categorize blood into four types: Blood Typing • ABO blood group system – The ABO system is the most important blood-group system in human-blood transfusion A B AB O Blood Typing • Examples: – Blood Group A • If you belong to the blood group A, you have A antigens on the surface of your red blood cells and B antibodies in your blood plasma Blood Typing • Examples: – Blood Group B • If you belong to the blood group B, you have B antigens on the surface of your red blood cells and A antibodies in your blood plasma Blood Typing • Examples: – Blood Group AB • If you belong to the blood group AB, you have both A and B antigens on the surface of your red blood cells and no A or B antibodies in your blood plasma. Blood Typing • Examples: – Blood Group O • If you belong to the blood group O, you have neither A or B antigens on the surface of your red blood cells and but you have both A and B antibodies in your blood plasma • How can you tell if antigens are present? • Agglutination is the clumping of particles. The clumping together of red blood cells usually in response to a particular antigen ( virus or bacteria). Blood Type • Eldon Card for testing blood type • The Agglutinated circle shows you what blood type you are. • Blood group A • No agglutination *With agglutination Blood Typing • Blood types are inherited and represent contributions from both parents Blood Typing • Rh blood group system • The Rh system is the second most significant blood-group system in human-blood transfusion • 1937 –Rhesus monkeys Blood Typing • Have the antigen Rh + • Don’t have the antigen Rh – • Important? » Erythroblastosis fetalis Biotech • Gel Electrophoresis – a process which enables the sorting of molecules based on size Biotech • Reading and autoradiograph – Who done it? Biotech • Reading and autoradiograph – Paternity testing Biotech Reading and autoradiograph (Suspect 2 is guilty) Biotech Reading and autoradiograph