THIS IS With Your Host... History of Biotech Biotech Methods Model Organisms Model Organisms II DNA DNA II 100 100 100 100 100 100 200 200 200 200 200 200 300 300 300 300 300 300 400 400 400 400 400 400 500 500 500 500 500 500 Which is not considered a major stage of biotechnology: A. Ancient Biotechnology B. Classical Biotechnology C. Contemporary Biotechnology D. Modern Biotechnology A 100 Which is not considered a major stage of biotechnology: C. Contemporary Biotechnology A 100 Why was Louis Pasteur’s discovery important? A. Linked microbes to disease B. Developed Pasteurization C. Microbe growth due to biogenesis D. All of the above A 200 Why was Louis Pasteur’s discovery important? D. All of the above A 200 WWII led to this advancement in classical biotechnology: A. mass production of influenza vaccine B. treatment of polio C. mass production of penicillin D. bio-warfare A 300 WWII led to this advancement in classical biotechnology: C. Mass production of penicillin http://www.history.com/shows/modern-marvels/videos/inventions-of-war-penicillin#inventions-of-war-penicillin A 300 A metabolic process in which organisms convert sugar to alcohols/acids: A. Lactation B. Fermentation C. Pasteurization D. Acidification A 400 A metabolic process in which organisms convert sugar to alcohols/acids: B. Fermentation A 400 Which historical stage of biotechnology advanced the manipulation of genetic information from organisms. A. Ancient B. Contemporary C. Classical D. Modern A 500 Which historical stage of biotechnology advanced the manipulation of genetic information from organisms. D. Modern A 500 Convert 30 µL to mL A. .030 L B. .30 mL C. 3.0 mL D. .030mL B 100 Convert 30 µL to mL D. .030 mL B 100 What is a solution? A. clear liquid B. 2 or more substances that can be separated C. 2 substances where solute completely dissolves into solvent D. All of the above B 200 What is a solution? C. 2 substances where solute completely dissolves into solvent * Cannot be separated B 200 What pipette would you use for a volume of 45 µL? A. B. C. D. H10 H100 H1000 Graduate Cylinder B 300 What pipette would you use for a volume of 45 µL? B. H100 B 300 What is a semi-permeable membrane? A. allows passage of all molecules B. allows passage of some molecules based on size C. allows only large molecules to pass through D. Does not allow any molecules to pass through B 400 What is a semi-permeable membrane? B. allows passage of some molecules based on size Semi-permeable membranes are selective allowing small molecules to diffuse through but larger molecules cannot . Cell membranes are semi-permeable B 400 How much CuSO4 is needed to make 50 mL of 5% CuSO4. A. 250 g CuSO4 B. 25 g CuSO4 C. 2.5 g CuSO4 D. .25 g CuSO4 B 500 How much CuSO4 is needed to make 50 mL of 5% CuSO4. C. 2.5 g CuSO4 B 500 Prokaryotes differ from eukaryotes due to: A. No nucleus B. No membrane bound organelles C. simple complexity D. All of the above C 100 Prokaryotes differ from eukaryotes due to: D. All of the above C 100 What are stem cells? A. cells that only come from embryos B. undifferentiated cells that can give rise to specialized cells C. cells that can cause cancer D. plant cells C 200 What are stem cells? B. undifferentiated cells that can give rise to specialized cells C 200 Why would the yeast grown during the Bacteria Growth Lab grow more in the 35o range? C 300 Why would the yeast grown during the Bacteria Growth Lab grow more in the 35o range? Compared to the other temperatures tested, 35o was closer to body temperature in which bacteria thrive C 300 DAILY DOUBLE C 400 What are induced pluripotent stem cells? A. reprogrammed adult stem cells B. embryonic stem cells coaxed to form specialized adult stem cells C. lab grown embryonic stem cells D. All of the above C 400 What are induced pluripotent stem cells? A. Reprogrammed adult stem cells Scientists continue to be excited of the future possibilities of induced pluripotent stem cells as they can offer an alternative to using embryonic stem cells. C 400 The Biuret test is used to test the presence of: A. Carbohydrates (sugars) B. Lipids C. Protein D. Starch C 500 The Biuret test is used to test the presence of: C. Protein. Positive Test: Bright blue to Purple C 500 What is the biggst issue with using embryonic stem cells? A. Ethical debate regarding the destruction of embryos B. cost C. long processing time D. limited stem cell lines D 100 What is the biggst issue with using embryonic stem cells? A. Ethical debate regarding the destruction of embryos. ~ All options listed are negative issues in using embryonic stem cells, however the ethical debate continues to be a hot topic that can impact funding for future research D 100 What test would you use to test for carbohydrates? A. biuret B. benedicts C. iodine D. Sudan IV D 200 What test would you use to test for carbohydrates? B. Benedicts Positive test: Blue to orange D 200 What is the gel like substance used to grow bacteria called? A. gel B. nutrients C. media D. agar D 300 What is the gel like substance used to grow bacteria called? D. agar D 300 Which organism is the most common model organisim used in biotechnology? A. Bacteria B. Worms C. flies D. monkeys D 400 Which organism is the most common model organisim used in biotechnology? A. bacteria D 400 What are knock out mice? A. Mice that are used to test drugs B. Mice that have a specific gene deactivated to determine gene function C. Mice who have altered genomes D. Mike Tyson’s pet mice D 500 What are knockout mice? B. Mice that have a specific gene deactivated to determine gene function D 500 What is the shape of DNA? A. Alpha helix B. Single helix C. Double helix D. Circular E 100 What is the shape of DNA? C. Double Helix E 100 What does DNA stand for? A. Deoxyribonuclear Acid B. Deoxyribose Acid C. Deoxyribosenucleic Acid D. Deoxyribonucleic Acid E 200 What does DNA stand for? D. Deoxyribonucleic Acid E 200 This molecule is important in linking the backbone of DNA. A. Carbon B. Phosphate C. Oxygen D. Nitrogen E 300 This molecule is important in linking the backbone of DNA. B. Phosphate E 300 What is the name of this molecule? A. DNA Polymerase B. Okazaki Fragment C. Helicase D. RNA Primer E 400 What is the name of this molecule? C. Helicase- Unzips DNA E 400 What direction does DNA replicate? A. 3’ B. 5’ C. 3’ 5’ D. 5’ 3’ E 500 What direction does DNA replicate? D. 5’ 3’ E 500 What is the complimentary DNA sequence to TGTACA? A. B. C. D. AGACTA CGATGG ACATGT ACACGT F 100 What is the complimentary DNA sequence to TGTACA? C. ACATGT A=T C=G F 100 Nucleic acids with two rings are known as: A. Purines B. Pyrimidines C. DNA D. Cyclic carbons F 200 Nucleic acids with two rings are known as: A. Purines F 200 Which experiment led to the discovery of a transforming factor (DNA)? A. Griffith B. Avery C. Pasteur D. Hershey and Chase F 300 Which experiment led to the discovery of a transforming factor (DNA)? A. Griffith F 300 Why was alcohol used to precipitate DNA from solution? A. Alcohol is polar like DNA B. DNA prefers the pH of alcohol C. Alcohol is nonpolar D. All of the above F 400 Why was alcohol used to precipitate DNA from solution? C. Alcohol is nonpolar. DNA by nature is a polar molecule. Alcohol is non-polar. When DNA comes into contact with alcohol, it will no longer mix and will precipitate out of solution F 400 What was the missing key evidence that Watson and Crick used in determining DNA structure. A. Franklin’s X-Ray Crystallagraphy photo B. Gel electrophoresis results C. Electron microscope picture of DNA D. Hershey and Chase results F 500 What was the missing key evidence that Watson and Crick used in determining DNA structure. A. Franklin’s X-Ray Crystallagraphy photo Photo 51 F 500 The Final Jeopardy Category is: DNA (insert evil maniacal laugh here…) (Please record your wager.) Click on screen to begin Samples found 1 2 3 Unknown Suspects 4 5 6 7 Based on the Gel Electrophoresis results, is it possible to determine who did it? Click on screen to continue Samples found 1 2 3 Unknown Suspects 4 5 6 7 Who did it? Suspect 6, all bands match Thank You for Playing Jeopardy!