OIE Reference Laboratory Reports
Activities in 2011
Name of disease (or topic) for
which you are a designated OIE
Reference Laboratory:
Address of laboratory:
Foot and mouth disease
Regional Reference Laboratory for FMF in South East Asia
National Institute of Animal Health
Department of Livestock Development
Pakchong, Nakhonratchasima 30130
THAILAND
Tel.:
+66 44 279112, +66 44 313869
Fax:
+66 44 314889, +66 44 313869
e-mail address:
website:
Name (including Title and
Position) of Head of Laboratory
(Responsible Official):
rrl@dld.go.th,
www. dld.go.th/rrl
Dr Wilai Linchongsubongkoch
Director, Regional Reference laboratory
for FMD since 1st January to 30
September 2011
Dr Panithan Thongtha
Acting Director, Regional Reference
Laboratory for FMD in South East Asia
Since 1st October 2011to the present
Name(including Title and
Position) of OIE Reference
Expert:
Name (including Title and
Position) of writer of this report
(if different from above):
Annual reports of OIE Reference Centres, 2011
Dr Wilai Linchongsubongkoch
Veterinary Expert, Consultant of
Department of Livestock Development
Dr Wilai Linchongsubongkoch
Dr Kingkarn Boosuya Seeyo
Veterinary officer, Regional Reference
Laboratory for FMD in South East Asia
1
Foot and mouth disease
Part I: Summary of general activities related to the disease
1. Test(s) in use/or available for the specified disease/topic at your laboratory
Test
For
Specificity
Total
Liquid phase blocking
ELISA (LP ELISA)
Antibody to structural
protein of FMDV
Sera from cattle,
buffaloes, pigs, goat,
12,246
Indirect blocking ELISA
Antibody to non-structural
protein (NSP)
Sera from cattle,
buffaloes, pigs
9,199
Antigen capture ELISA
Antigen
Infected tissue from
tongue epithelium and
foot and laboratory
samples
834
BHK-21 cell culture
Virus isolation
Filed isolate samples
398
RT-PCR
Antigen
Tissue samples and virus
isolation
354
Sequencing
Molecular analysis
Field isolate viruses
107
r-value by LP ELISA
Vaccine matching
Field isolate viruses
56
2. Production and distribution of diagnostic reagents
The diagnostic reagents produced by Regional Reference Laboratory for FMD in South East Asia (RRLSEA),
Pakchong, Thailand was mainly ELISA reagents use in ELISA technique, the number of reagents produced in the
year 2011 was shown in the table below:
Table 1 Quantity of FMD diagnostic reagents that has been produced at RRLFMD in year 2011
Item
1
Quantity (ml)
Rabbit trapping antibody to FMDV
type O,
type A
type Asia1
40
98
18
Guinea pig detecting antibody to FMDV
type O,
type A
type Asia1
112
205
81
Inactivated FMD antigen (concentrated and partial purified)
type O,
type A
type Asia1
200
240
200
4
Strong positive control serum to FMDV type O, A and Asia1
240
5
Weak positive control serum to FMDV type O, A and Asia1
240
2
3
2
Description
Annual reports of OIE Reference Centres, 2011
Foot and mouth disease
Table 2. Number of FMDV diagnostic reagents and reference materials from RRLSEA supplied at the national
and regional level for OIE member countries in 2011.
Type of reagents
Supplied nationally
and own lab
Supplied OIE
Members Countries
Total
Remarks
ELISA reagent for
FMDV Rabbit
trapping antibody for
type O, A, and Asia1
Type O = 23 sets
Type A = 33 sets
Type Asia1 = 23 sets
Type O = 9 sets
Type A = 9 sets
Type Asia1 = 9 sets
Type O = 32 sets
Type A = 42 sets
Type Asia1 = 32 sets
1 set can be
tested for
1000 samples
Guinea pig detecting
antibody for type O,
A and Asia1
Type O = 26 sets
Type A = 36 sets
Type Asia1 = 30sets
Type O = 9 sets
Type A = 9 sets
Type Asia1 = 9 sets
Type O = 35 sets
Type A = 45 sets
Type Asia1 = 39 sets
1 set can be
tested for
1000 samples
Concentrated
inactivated (20X)
antigen type O, A,
Asia1
Type O = 165 ml
Type A = 210 ml
Type Asia1 = 180 ml
Type O = 9 ml
Type A = 9 ml
Type Asia1 = 9 ml
Type O = 174 ml
Type A = 219 ml
Type Asia1 = 189 ml
Control serum for
C++, C+ and C-
C++ = 660 ml
C+ = 510 ml
C- = 495 ml
C++ = 9 ml
C+ = 9 ml
C- = 9 ml
C++ = 669 ml
C+ = 519 ml
C- = 504 ml
Part II: Activities specifically related to the mandate
of OIE Reference Laboratories
3.
International harmonisation and standardisation of methods for diagnostic testing or the
The standardisation of test method for FMD diagnosis using antigen capture ELISA, LP ELISA, NSP test and
virus isolation test have been harmonized since the first lab network meeting in 2005 and the standard operation
procedure (SOP) of those test methods have been distributed to all member countries to use in their routine assays.
a)
Establishment and maintenance of a network with other OIE Reference Laboratories
designated for the same pathogen or disease and organisation of regular inter-laboratory
proficiency testing to ensure comparability of results
SEACFMD Laboratories network meeting has been organized annually under the support by OIR-SRR and
OIE-RCU, the RRLSEA acts as the leader and co-organizer of this network. The network activities in the
year 2011 as this follow:

The 6th meeting of SEACFMD laboratory network meeting and joint EpiNet meeting was held in
Pakchong, Thailand during 2-3 March 2011. Head of FMD national laboratories and Head of
epidemiologists from Cambodia, Brunei, China, Lao PDR, Malaysia, Myanmar, Philippines,
Indonesia, Vietnam, Thailand including the Representative from FAO, OIE-SRR were participated.
There was a special lecture on transportation of infectious substances by Dr Wilai
Linchongsubongkoch, an OIE Expert. In addition, a packing of specimen using standard container
approved by IATA has been demonstrated and practiced by all participants to have an experience and
discuss on constrain of airline shipment. The protocol sample submission to the reference laboratory
has been prepared and distributed to all member countries to use as a guidance.

The second round of 2011_ inter-laboratory comparison testing on foot and mouth disease (FMD)
ELISA typing, FMD serology by LP ELISA and NSPs test has been organized by RRLSEA,
Pakchong, Thailand during March to August 2011. Totally 17 FMD laboratories within Thailand and
SEACFMD countries were participated. The reports of 2011_inter-laboratory comparison has been
prepared and distributed to all participating laboratories and National Coordinators in August 2011
during the 14th National Coordinator Meeting of the SEACFMD in Hanoi, Vietnam during 12-16
August 2011.
Annual reports of OIE Reference Centres, 2011
3
Foot and mouth disease
b)
4.
Organisation of inter-laboratory proficiency testing with laboratories other than OIE
Reference Laboratories for the same pathogens and diseases to ensure equivalence of
results

RRLSEA participated in Foot and Mouth Disease and Swine Vesicular Disease (SVD) Proficiency
studies 2011 by antigen capture ELISA, LP ELIA and NSP ELISA and RT-PCR test. The PT
programme was organized by WRL, Pirbright Laboratory, UK during August – October 2011. The PT
results has been sent to WRL since October 2011.

RRLSEA has sent a panel of FMD samples annually to World Reference Laboratory (WRLFMD),
Pirbright Laboratory, UK. Totally 34 FMD samples were submitted to WRL in July 2011 for FMDV
serotype confirmation and strain characterisation, the diagnostic results were compared between
RRLSEA, Thailand and WRL, UK. Details of 34 samples were from Thailand = 17, Vietnam = 6, Lao
PDR = 4 and Sri Lanka = 2 respectively. The diagnostic results from both laboratories gave a
similarly results or indicted as 100 % agreement.
Preparation and supply of international reference standards for diagnostic tests or vaccines and
supply of international reference standards for diagnostic tests or vaccines
Preparation of reference materials such as rabbit trapping antibodies, guinea pig detecting antibodies, inactivated
FMD antigen, positive control serum and negative control serum for verification and validation assay in order to
meet the standard of ISO 17025:2005 regulation. These reference materials were supplied to RRLSEA laboratory,
8 regional laboratories within Thailand and OIE member countries, such as Cambodia, Myanmar, Vietnam, Lao
PDR, Sri Lanka as already listed in the table 2 of item No. 2.
5.
Research and development of new procedures for diagnosis and control
Several projects have been conducted in this year and in progress, as this follow:
Collaboration with the RRLSEA, Pakchong, Thailand and Australian Animal Health Laboratory (AAHL,
Geelong, Australia, entitled: Foot and Mouth Disease Risk Management for Australia and South East Asia,
duration 5 years, from 2011 -2015.
Development of real time PCR technique for foot and mouth disease diagnosis, now the test has been used as
routine diagnosis for supporting the ELISA typing test.
On going Serosurveillance of foot and mouth disease in elephants in Thailand (3 years project, 2010- 2012)
Serosurveillance of foot and mouth disease in sheep and goat in Thailand (2 year project, 2012-2013)
On going vaccine matching test (r-value) and molecular epidemiology analysis of foot and mouth disease
viruses causing outbreak in Thailand and SEA region.
6.
Collection, analysis and dissemination of epizootiological data relevant to international disease
control
Not done.
7.
Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the
pathogen and the disease concerned
There were several activities has been conducted annually in order to maintain the international standard of quality
assurance system, Biosfety and biosecurity system. As this follow:

4
To maintain the ISO /IEC 17025:2005 Certified on Veterinary Laboratory testing for FMD diagnosis in the
scopes of Antigen typing test, LP ELISA test and Non-structural protein test, by conducting the internal audit
or assessment once a year by Accreditation Boby in Thailand, other activities related to ISO 17025 regulation
such as calibration of essential equipment, verify standard materials regularly, staff training and regularly
update the quality manual document under the ISO 17025 standard.
Annual reports of OIE Reference Centres, 2011
Foot and mouth disease
8.

To maintain the biosafety and biosecurity system in the laboratory by conducting the annual maintenance of
BSL-3 microsecurity system such as testing of HEPA Filter efficacy, negative pressure system, Air-locked
system, Pneumatic Logic Control (PLC) system, Exhaust system, Bio-waste treatment system, checking of 2door autoclave and also staff training in Biosecurity system regularly.

Participating in the Biorisk Management Programme organized by the Department of medical Sciences,
Ministry of Public Health, This activity is to establish and implement the biosafety and biosecurity in the
laboratory for animal health and human health in Thailand to work together in order to establish the One
Health Concept in near future.
Provision of consultant expertise to OIE or to OIE Member Countries
8.1 Dr Wilai Linchongsubongkoch, has been worked with Department of Livestock Development (DLD) for the
contribution and review of text book on OIE manual of diagnostic tests and vaccine for Terrestrial animals on
chapter 2.1.5 Foot and Mouth Disease and Terrestrial Animal Health Code.
8.2 Dr Wilai Linchongsubongkoch, has been attending the OIE Commission and Ad Hoc Group on Biosafety
and Biosecurity in Veterinary Laboratories, the AHG worked on reviewing the OIE Terrestrial manual of the
chapter 1.1.1 ‘ Collection and shipment of diagnostic specimens’ and chapter1.1.2 ‘Biosafety and biosecurity
in the veterinary microbiology laboratory and animal facilities’ at OIE Headquarters during 19 -21 September
2011.
8.3 Dr Wilai Linchongsubongkoch has been attending OIE Sub-Commissions annual meeting for foot and mouth
disease control campaign in South East Asia and China (SEACFMD) and others OIE meeting as a technical
resource person.
8.4 Dr Wilai Linchongsubongkoch has been attending the second project Steering Committee for phase II on
Transboundary Animal Disease Control for Poverty Reduction in the Greater Mekong Subregion
(GCP/RAS/233/ASB) as an technical resource person, at FAO Office, Bangkok, Thailand during 19 -30 June
2011.
8.5 Dr Wilai Linchongsubongkoch has been attending the meeting of technical committee of National Bureau of
Agriculture Commodity and Food Standard (ACFS), Thailand on reviewing the standard procedure of foot
and mouth disease diagnostic manual, during July – September 2011.
9.
Provision of scientific and technical training to personnel from other OIE Member Countries
9.1 Training of RRL staff in 2011
9.1.1
9.1.2
9.1.3
9.1.4
9.1.5
9.1.6
9.1.7
9.1.8
9.1.9
9.1.10
Total 4 persons have been train on Internal audit for ISO 17025:2005, during 22-23 February 2011
Total 5 persons have been trained on Statistic for Analysis testing, during 8-10 March 2011
Total 5 persons have been train on Analysis Instrument Certification, on 19 April 2011
Total 4 persons have been trained on Research methodology on 24 March 2011
Total 5 persons have been trained on Knowledge Management (KM). During 10-11 May 2011
Total 8 persons have been trained on Biosafety and Bosecurity during 23-14 June 2011
Dr Kingkard Boonsuya Seeyo has been participated in Laboratory Training Course on FMD in Cattle at
Yunan Animal Science and Veterinary Institute, Yunan province, P.R. China, during 9-10 June 2011. The
training under the FAO-ADB supported.
Dr Kingkarn Boonsuya Seeyo has been trained on Laboratory Biorisk Management Awareness and
Transportation of Infectious Substance in July 2011.
Dr Kingkarn Boonsuya Seeyo was attending the FMD vaccine matching training course at World
Reference Laboratory (WRL), Pirbright, UK, during 7-11 October 2011.
Mr. Jarouy Yothakeaw received training on Advance Biorisk Training at Australian Animal Health
Laboratory(AAHL), Geelong, Australia during 21-25 November 2011, the training was Supported by
FAORAS and USAID.
9.2 FMD Training to OIE member in 2011
9.2.1
Total 8 persons from OIE –SRR, Bangkok has visited RRLSEA laboratory and the BSL-3 facilities also
there was a discussion and exchange FMD information.
Annual reports of OIE Reference Centres, 2011
5
Foot and mouth disease
9.2.2
9.2.3
9.2.4
Two trainees from Sri Lanka, Dr Eranga Abeyratna and Mr. Ha Wijithasiri, from Research Institute,
Department of Animal Production and Health, Sri Lanka received training on FMD vaccine production,
quality control and FMD diagnosis, during 17 January 2011 to 28 February 2011.
Two laboratory staff from Department of Livestock and Fisheries (DLF), Lao PDR received training on
Bio-safety Management in Laboratory for 2 weeks, during 1-12 August 2011. The training was under
FAO supported.
Total 5 Veterinary officers and with Scientists from Yunan Animal Science and Veterinary Institute,
Yunan province, P.R. China received training on FMD diagnosis for 1 week during 29 August to 2nd
September 2011 under the project on The Exchange of diagnostic techniques for transboundary animal
disease.
10. Provision of diagnostic testing facilities to other OIE Member Countries
RRLSEA have provided the diagnostic services to OIE member countries on antigen typing test, LP ELISA, NSP
test, RT-PCR, strain characterisation by r-value and sequencing test, details as this follow.
Country
Type of specimen
Test method
Total samples
Cambodia
Epithelium tissue and tissue
extraction fluid
Serum sample
Antigen typing, RT-PCR
r-value and sequencing
31
LP ELISA and NSP
80
Lao PDR
Isolate virus
Antigen typing, RT-PCR
and sequencing
20
Vietnam
Epithelium tissue and Isolate virus
Antigen typing, RT-PCR
r-value and sequencing
27
Sri Lanka
Epithelium tissue and Isolate virus
Antigen typing, RT-PCR
and sequencing
4
11. Organisation of international scientific meetings on behalf of OIE or other international bodies
The 6th SEACFMD Laboratories Network and joint FMD EpiNet Meeting was co-organized with OIE-SRR under
the support of OIE-SRR at Pakchong, Nakhonratchasima, Thailand during 2-3 March 2011.
12. Participation in international scientific collaborative studies
12.1 The RRLSEA has been conducted cooperative work and exchange FMD diagnostic information and samples
with World Reference Laboratory for FMD (WRL), Pirbright, UK by sending a panel of FMD specimens and
isolation samples to WRL annually. This year totally 34 samples were sent to WRL in July 2011 for the
confirmation of typing ELISA and strain characterisation by r-value and sequencing.
12.2 The RRLSEA participated in 2011 External Quality Assurance Exercises for proficiency testing in serology
for foot-and-mouth disease. The programme was organized by Dr Jef Hammond, WRL, Pirbright Laboratory,
UK during August –October 2011. A panel Non-infectious unknown samples (6 samples), were received for
antigen detection as well as a panel of non-infectious sera for serology test by LP ELISA and NSP test (6
samples), The results was sent to WRL by October 2011 and final report is on-going evaluated.
12.3 The RRLSEA organized the inter-laboratory comparison programme for FMD serology test and antigen
detection, Totally 17 laboratories were participated in this programme exercise, which were 9 Laboratories
from South East Asia countries and 8 FMD Laboratories within Thailand. A set of non-infectious reagents
consist of 5 unknown serum samples and 10 unknown inactivated agents were distributed on date 3 rd March
2011 to all participating laboratories to use for LP ELISA, NSP test and antigen typing test respectively. The
inter-laboratory samples has been analysed, the completed report has been prepared and distributed to all
participating laboratories within September 2011
12.4 On-going collaboration with the RRLSEA, Pakchong, Thailand and Australian Animal Health Laboratory
(AAHL, Geelong, Australia, entitled: Foot and Mouth Disease Risk Management for Australia and South
6
Annual reports of OIE Reference Centres, 2011
Foot and mouth disease
East Asia, duration 5 years, from 2011 -2015.
13. Publication and dissemination of information relevant to the work of OIE (including list of
scientific publications, internet publishing activities, presentations at international conferences)

-
-
-
-
-

-
Presentations at international conferences and meetings
Linchongsubongkoch, L. was attended the final Thailand joint coordinating committee meeting (JICA
ADB2-Thailand project), 14 January 2011, Bangkok, Thailand.
Linchongsubongkoch, L. was attended the One Health Congress, 14-16 February 2011, Melbourne,
Australia.
Linchongsubogkoch, L. was invited to give presentation in the 6th SEACFMD Laboratories Network and
joint FMD EpiNet meeting, Pakchong, Nakhonratchasia, Thailand during 2-3 March 2011.
Linchongsubogkoch, L. was invited to give presentation and attending the 17th Meeting of the OIE SubCommission for Mouth Disease Foot and Mouth Disease in South East Asia, Bali, Indonesia, during 7-11
March 2011.
Aunpomma, D., Thongtha, P. and Seeyo Kingkarn Boonsuya were attended the 6th SEACFMD Laboratories
Network and joint FMD EpiNet meeting, Pakchong, Nakhonratchasia, Thailand during 2-3 March 2011.
Aunpomma, D. and Seeyo Kingkarn Boonsuya were attended in the 17th Meeting of the OIE SubCommission for Mouth Disease Foot and Mouth Disease in South East Asia, Bali, Indonesia, during 7-11
March 2011.
Linchongsubongkoch, L was invited to give presentation in STANDZ Launching and Steering Committee,
STANDZ Monitoring and Evaluation Workshop and 14th meeting of SEACFMD National Coordinators
(NC), Hanoi, Vietnam during 12-16 September 2011.
Linchongsubongkoch, L was invited to attend the OIE Commission and Ad Hoc Group on Biosafety and
Biosecurity in Veterinary Laboratories, OIE, Paris during 19-21 September 2011.
Dr Panithan Thongtha and Dr Kingkarn Boonsuya Seeyo were invited to give a presentation on the 3rd
Central Laboratory Directors Meeting, KL, Malaysia 18-21 October 2011.
Linchongsubogkoch, L. And Dr Kingkarn Boonsuya seeyo were participated and given the presentation in
The 7th OIE/FAO FMD Reference Laboratories Network Meeting, Pirbright laboratory, UK, during 13-16
November 2011. Organized by OIE/FAO.
Linchongsubogkoch, L. was invited to give presentation in the Inception meeting of the OIE/JTF project for
FMD control in Asia, Tokyo, Japan, 13-14 December 2011.
Scientific publications in peer-reviewed journals
Linchongsubongkoch, L., Aunpomma, D., Singkleebuth, S., Samanit, J., Chareonpol, P. and Thongtha, R.
2011. Quality assurance for foot and mouth disease laboratories network. Annual technical meeting of the
National Institute of Animal Health, Surin province, Thailand during 21-24 June 2011.

Scientific publications in peer-reviewed journals
-
Linchongsubongkoch was requested from OIE to review scientific paper for publishing in OIE journal
entitled ‘ Epidemiology of foot and mouth disease in Uttar Pradesh state, India 2000-2009’ Authors by Amit
Kumar Verma, Sahzad and S. K. Yadav, Department of Veterinary Epidemiology and Preventive Medicine,
DUVASU, Mathura, Uttar Pradesh, India

Other communications
-
Technical consultant and assistant on FMD subjects to universities and institutes both in country and OIE
member countries such as analysis and validation assay on project titled Development of recombinant 3ABC
non-structural protein based ELISA to differentiate vaccinated from infected animals
Consultant and gave advices to FMD laboratories within Thailand and OIE member countries on FMD
diagnostic testing and quality assurance control system also other subjects related to FMD.
Assist and coordinate with OIE member countries (Myanmar, Cambodia, Lao PDR and Vietnam) in
preparing an essential document, packing of infectious substances and transportation of specimen to
Reference laboratory by International Airline complied with IATA regulation and using standard container
approved by UN or IATA.
_______________
-
Annual reports of OIE Reference Centres, 2011
7