Bacterial lipopolysaccharide differently modulates steroidogenic
enzymes gene expressions in the brain and testis in rats
By:Sadasivam, M (Sadasivam, Mohanraj)[ 1 ] ; Ramatchandirin, B (Ramatchandirin, Balamurugan)[ 1 ] ; Ayyanar,
A (Ayyanar, Ananth)[ 1 ] ; Prahalathan, C(Prahalathan, Chidambaram)[ 1 ]
NEUROSCIENCE RESEARCH
Volume: 83
Pages: 81-88
DOI: 10.1016/j.neures.2014.02.011
Published: JUN 2014
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Abstract
Bacterial lipopolysaccharide (LPS) is a major component of the cell wall of gram negative bacteria contributing to the
pathogenesis of bacterial infection, in particular in those diseases affecting central nervous system and reproductive
tissues. The present work is an attempt to study the regulation of steroidogenic enzymes gene expression in the brain
and testis in LPS induced rats. Adult male albino rats were administered LPS (5 mg/kg BW) to induce acute
inflammation. LPS administration induced severe oxidative damage in the brain and testicular tissue which was evident
from decreased activities of enzymic antioxidants and increased lipid peroxidation levels. The mRNA expression of 3
beta-hydroxysteroid dehydrogenase (3 beta-HSD), 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) and
androgen receptor corepressor-19 kDa (ARR19) in the brain and testis were determined. The mRNA expression of 3
beta-HSD and 17 beta-HSD was increased in the brain with significant decrease in the testis at 24 h and 48 h in LPS
treated animals. The results also demonstrated an interesting finding that LPS treatment completely represses ARR19
in the brain, while not in the testis. These findings show ARR19 might play a crucial role in regulation of neuronal and
testicular steroidogenesis in inflammatory diseases. (C) 2014 Elsevier Ireland Ltd and the Japan Neuroscience Society.
All rights reserved.
Keywords
Author Keywords:Lipopolysaccharide; 3 beta-HSD; 17 beta-HSD; ARR19; Steroidogenesis
KeyWords Plus:TUMOR-NECROSIS-FACTOR; LEYDIG-CELL STEROIDOGENESIS; PITUITARY-TESTICULAR
AXIS; SEPTIC SHOCK; COLORIMETRIC ASSAY; OXIDATIVE STRESS; IN-VIVO; INTERLEUKIN1; SUPPRESSION; SEPSIS
Author Information
Reprint Address: Prahalathan, C (reprint author)
Bharathidasan Univ, Dept Biochem, Tiruchchirappalli 620024, Tamil Nadu, India.
Addresses:
[ 1 ] Bharathidasan Univ, Dept Biochem, Tiruchchirappalli 620024, Tamil Nadu, India
E-mail Addresses:prahalath@gmail.com
Funding
Funding Agency
Department of Biotechnology (DBT)
Indian Council of Medical Research (ICMR), New Delhi, India
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Grant Number
Publisher
ELSEVIER IRELAND LTD, ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000,
IRELAND
Categories / Classification
Research Areas:Neurosciences & Neurology
Web of Science Categories:Neurosciences
Document Information
Document Type:Article
Language:English
Accession Number: WOS:000344205000008
PubMed ID: 24594480
ISSN: 0168-0102
eISSN: 1872-8111
Other Information
IDS Number: AS3WC
Cited References in Web of Science Core Collection: 35
Times Cited in Web of Science Core Collection: 2