Structure and morphology of fungi Mrs. Dalia kamal Eldien Msc in Microbiology Lecture NO (1) Objective Definition of mycology Beneficial Effects of Fungi Harmful Effects of Fungi General properties of fungi Morphology of fungi Introduction The term "mycology" is derived from Greek word "mykes" meaning mushroom. The mycology is the study of fungi The medical mycology is a branches that deal with the study of pathogenic fungi to man and animal, the fungal infections now a day are in increasing rate Beneficial Effects of Fungi 1-Decomposition : lower fungi produce enzyme break down the organic dead materials 2-Biosynthetic factories: The fermentation property is used for the industrial production of alcohols, fats, citric and gluconic acids. 3-Important sources of antibiotics: such as Penicillin(Penicillium chrysogenum). Cephalosporin(Cephalosporium acremonium) 5- Model organisms for biochemical and genetic studies e.g: Neurospora crassa, Saccharomyces cerviciae is extensively used in recombinant DNA technology. 6-Some fungi are eatable e.g Mushrooms 7-Yeasts used in bakery, yogurt, cake…….. Mushrooms Cake& cheese (yeast) Harmful Effects of Fungi 1. Destruction of food, lumber, paper, and cloth. 2. Food poisoning, toxins produced by poisonous mushrooms (Mycetism and Mycotoxicosis). 3. Plant diseases. 4. Damage of agriculture produce such as vegetables and cereals in the godown. 5. Damage the products such as magnetic disks, glass lenses, marble statues, bones and wax. 6. Animal and human diseases. Wood decaying fungus Destruction of food by fungi General properties of fungi They are eukaryotic; cells contain membrane bound cell organelles including nuclei, mitochondria, Golgi apparatus, endoplasmic reticulum, lysosomes etc. They reproduce by mitosis. Have Ergosterols in their membranes(in contrast to cholesterol found in mammalian cells) and possesses 80S ribosome's. Have a rigid cell wall made of chitin and they are non-motile, this feature that separates them from animals. Fungi are osmiotrophic; they obtain their nutrients by absorption Are chemoheterotrophs (require organic compounds for both carbon and energy sources) and fungi lack chlorophyll and are therefore not autotrophic. All fungi require water and oxygen and there are no obligate anaerobes. Typically reproduce asexually and/or sexually . Food storage is generally in the form of lipids and glycogen. Eukaryotic cell Reproduction in fungi Fungi reproduce asexually and sexually Asexual reproduction is the commonest mode in most fungi Asexual spores are produced following mitosis where as sexual spores are produced following meiosis. Morphology of fungi Fungi exist in two fundamental forms; The filamentous (hyphae) Single celled budding forms (yeast). Filamentous fungi The main structure element are cylindrical tube like structures refer to as hypha(e) Amass of hyphae is known as mycelium. The hyphae may be branched or unbranched. They may be septate or aseptate (coenocytic), hyphae usually have cross walls that divide them into numerous cells, these cross walls, called septa have small pores through which cytoplasm is continuous throughout the hyphae. Septate hyphae Non-Septate Hyphae (Coenocytic) Mycelium are divided in to 3 parts 1. Vegetative mycelium are those that penetrates the surface of the medium or substrate and absorbs nutrients. 2. Aerial mycelium are those that grow above the agar surface 3. Fertile mycelium are aerial hyphae that bear reproductive structures • Example : Moulds, Dermatophytes Yeasts Yeasts are unicellular cells with different shape and size round, oval or elongated. They reproduce by budding, which result in blastospore In some cases, as the cells buds the buds fail to detach and elongate thus forming a chain of elongated hyphae like filament called pseudohyphae, this property is seen in Candia albicans. True yeasts such as Saccharomyces cerviciae don't produce pseudohyphae. Some yeast are produce polysaccharide capsule Budding in yeast Yeast under light microscope Dimorphic fungi Some species of fungi have the ability to shift from the yeast form to the filamentous (mold) form and vice versa. These fungi are dimorphic. They can exist in filamentous or yeast form depending on the condition of growth, mainly the temperature 26 C = filamentous form 37 C= yeast form Example: Histoplasma Capsulatum