The antibacterial effect of 25wssw managed rabbit serum
FY SUN1 H LIU1 Y LIU1 L DU1 Q ZHAO1 DY YUAN1SM ZHANG2
1. basic research of life science laboratory of Tibet National college , medicine
screening laboratory
2. Medicine dept. of Shanghai Jiaotong Univ.
FY SUN, Email: xzmysfy@163.com. TEL: 18691096609
Abstract
OBJECTIVE：To study the antibacterial effect of Tibetan medicine 25WSSW
managed rabbit serum on Staphylococcus Aureus and Escherichia Coli. METHOD:
Dissolve 22WSSW in 30℃ warm water. Set human usual dose as medium dose,
tripling dilute the usual dose as small dose, 3 times the concentration of usual
dose as large dose. Put 30 male rabbits into 5 groups randomly: DXM group, NS
group, 25WSSW small dose group(0.07g/kg), 25SWWS medium dose
group(0.2g/kg), 25WSSW large dose group(0.6g/kg). Administration 5 times by
garage, fasting 12h before the fifth time of administration, water supplied freely.
1h before the administration inject 100 ug/kg endotoxin in rabbits via ear veins.
On DXM group, there only needs 1 time administration at the same time with last
administration on Tibetan medicine group. rabbits aneasthesia when 6h after
injecting endotoxin, separate serum from blood samples, which had been taken
from artery to detect endotoxin quantitatively by endotoxin detector. Perforate
on Staphylococcus aureus/ Escherichia Coli managed agar plates respectively,
and put serum sample into it, measure the area of inhibition zone by cross
method. RESULT: Compared with negative control group, endotoxin
concentration at medium group increased obviously, but endotoxin
concentration decreased obviously compared with positive control group
(P<0.05). The area of inhibition zone to Staphylococcus aureus on DXM group
reduced obviously, but has no significant difference on other groups. The area of
inhibition zone to Escherichia Coli on DXM group reduced obviously, but
enlarged on medium/large/small dose group obviously, p<0.05. CONCLUSION:
25WSSW has killing effect to Escherichia Coli, but has no effect to Staphylococcus
Aureus.
Key words: Tibetan medicine, endotoxin, Escherichia Coli, Staphylococcus
Aureus.
Fund project:
2013 Natural Science Foundation of Tibet science and Technology Department
2015 Key Natural Science Foundation of Tibet science and Technology
Department