Meiosis Lab: Notebook Format
1) Title/Date
2) Pre-lab
A) Key Concepts
B) Materials List
C) Expected Outcomes
3) Purposes (2-3)
4) Personal Account
A) Prediction re: recombination frequency
B) Procedure
-summarize procedure from handout, then…
-”to reduce challenges, we analyzed photos of prepared slides”
C) Data Table (Table 3.2 from handout)
5) Discussion
-Questions #2 & #3 from handout
-work backwards to answer #2. As drawn, you get 2:2:2:2. Follow
the tips of the chromosomes.
6) Conclusion
Meiosis Lab
Sordaria fimicola – fungus
Life cycle of Sordaria
Meiosis occurs IMMEDIATELY after zygote is formed
By using 2 different strains of Sordaria, we can see the products of meiosis
-Wild-type = black spores
-Mutant = tan spores.
-When the 2 strains mate, we can follow their chromosomes (spore color)
during meiosis.
4:4
 - mitosis here is unique to fungi
- The 4:4 pattern indicates NO crossing over has taken place.
2:2:2:2 or
2:4:2
The 2:2:2:2 or 2:4:2 pattern indicates crossing over has occurred.
Count 50 hybrid asci……with both mutant & WT spores (tan & black)
Complete Table 3.2
Answer questions 2 & 3 under Analysis….
these are your discussion questions
These Sordaria pictures can be found at:
http://www.fultonschools.org/teacher/tuckerjs/apbio/meiosis/Sordaria_Examples.htm