Pneumonia Barriers to infection: • Epiglottis: protects airway from aspiration. • Cough reflex*. • Mucociliary escalator. • Alveolar macrophage & antimicrobial. compounds: lysozyme, lactoferrin, complement and IgA. Pneumonia: Inflammation of the lung parenchyma: Inflammation of alveoli, alveolar ducts, bronchioles, and interstitial tissue of lung, induced by microbial invasion of natural barriers. Classification of pneumonia: • Clinical Classification: Acute: less than three weeks duration • Chronic pneumonias. Acute pneumonia is further classified according to place where it was acquired, source of transmission, and etiology. Classification of Acute Pneumonia: Community acquired: o Person to person Classical bacterial pneumonia Atypical bacterial pneumonia Viral pneumonia o Animal, or Environmental Exposure. Nosocomial acquired. Community-acquired acute pneumonia: Person-to-Person: A- Classic bacterial pneumonia: • Streptococcus pneumoniae. • Haemophilus influenzae. • Klebsiella pneumoniae. • Moraxella catarrhalis. • Aspiration pneumonia* (mixture of bacteria including gram negatives, anaerobes and Staphylococcus aureus). Person to Person: B- Atypical bacterial pneumonia: • Chlamydophila pneumoniae • Mycoplasma pneumoniae. C- Viral pneumonia: • Influenza virus type A, B, and C • Coronaviruses. • Others: RSV, measles , adenoviruses, CMV….. Environmental o o o o o Legionellosis. Tularemia. Plague. Q fever. Anthrax. or Animal Exposure: Streptococcus pneumoniae : Reservoir: nasopharynx of children and adults.(patients or asymptomatic carriers). Transmission: droplets inhalation. Pathogenesis and microbial virulence: o Colonization of the nasopharynx then spread to the bronchi and alveoli. o Polysaccharide capsule resist phagocytosis. o Production of pneumolysin: cholesterol-binding toxin; epithelial cell-damage. o Production of hydrogen peroxide; cell damage, and inhibition of other bacteria. o Inflammation: production of cytokines; TNFα,IL-1, IL-8 by alveolar macrophage (stages of pneumonia): Filling of alveoli by fluids*: many bacteria, and few inflammatory cells. Early consolidation* stage; infiltration of alveoli by neutrophils, activation of complement and CRP which interact with bacterial teichoic acid. (battle b/w the bacteria and the immune system). Late Consolidation stage: heavy infiltration by neutrophils which kill the microbe, (Hepatization). Infection eradication (resolution): Replacement of neutrophils by alveolar macrophages. Clinical presentation: Acute lobar pneumonia. Classic bacterial pathogenesis: Lobar Pneumonia Complications of Streptococcus pneumonia: Local complications: Pleural effusion; Outpouring of fluid into pleural space in 25% of cases. Empyema (pus in the pleura): in 1% of cases, require drainage of fluid. Systemic complications • Bacteremia (Pneumococcemia): through lymphatic vessels of the lung to thoracic duct. • Positive blood culture in only 25% of cases (transient bacteremia). • Defense: humoral factors and lymphatic system to remove the bacteria from the blood. • Meningitis esp. in splenectomy, sickle cell anaemia Diagnosis of S. pneumoniae: • Clinical specimens: Sputum, transtracheal aspirate, broncheoalveolar lavage and lung biopsy. • Direct Microscopy: Streptococcus pneumoniae are Gram positive lanceolate (lancet) shaped diplococci arranged in pairs or chains, and capsulated. • Cultural characteristics: Facultative anaerobic bacteria, alpha hemolytic on blood agar, optochin sensitive. Blood agar: α hemolysis & optochin sensitive Haemophilus influenzae -Gram negative coccobacilli, rod-shaped to pleomorphic. - Epiglottitis, tracheobronchitis and pneumonia. - Isolation of Haemophilus influenzae : -Aerobic or facultative anaerobic bacteria. -Isolated on chocolate agar with factor X (hemin) and factor V (NAD). -Satellitism phenomenon. Treatment of S. pneumoniae and H. influenzae: • Beta-lactam antibiotic. • If the patient is allergic to penicillin or the bacteria is not sensitive: macrolide or fluoroquinolones. • Penicillin-resistant streptococcus due to mutation in penicillin-binding protein by transformation. Vaccine: -Conjugated capsular antigen vaccines for S. pneumoniae and H. influenzae type b. Atypical bacterial pneumonia: Chlamydophila pneumoniae: • Infective stage: Elementary bodies. -Target cells: columnar epithelial cells, endothelial cells of the vessels and macrophages. - Receptor-mediated endocytosis (intracellular infection). - Lymphocytic infiltration; IF-γ creates persistence infection by slowing the growth of the RB. • Diagnostic stage: Reticulate bodies. Life cycle of C. pneumoniae Clinical presentation: Acute tracheobronchitis. Bronchopneumonia (patchy infiltrates on radiography). C. pneumoniae is associated with Coronary artery disease (CAD): o Adults with CAD have a high antibodies titer against this bacterium. o The microbe can be isolated from atherosclerotic lesions. o The microbe established CAD in animal model studies. Diagnosis: • Immunofluorescent microscopy for antigen and antibodies detection. • PCR. Treatment: - Macrolide: Long-term Cmax (maximum serum concentration) of azithromycin. - Or doxycycline for 7 days. - Pregnant women: erythromycin or azithromycin. Mycoplasma pneumoniae: • Smallest prokaryotes that lack cell-wall. • Infect mainly individuals aged 5-20 years old. • Pathogenesis: Tip structure mediated attachment to carbohydrate containing receptor on columnar epithelial cells The infection is not highly destructive but the ciliary function is impaired. Exotoxins: • ADP-ribosyltransferase: inhibition of neutrophils chemotaxis and phagocytosis. • Vacuolating toxin: apoptosis of ciliated cells. • Monocytic infiltration. (few neutrophils) Clinical presentation: • Tracheobronchitis (persistent dry cough). • Bronchopneumonia; (infiltration of monocytes with few neutrophils); patchy infiltrate on radiography. • In 50% of severe mycoplasma infections; mild-autoimmune hemolytic anemia* due to cold hemagglutinin formation. Complications: • Encephalitis, renal disease and arthritis (antibody complex), autoimmune thrombocytopenic purpura* erythema multiforme). Diagnosis: -Direct: PCR or Immunofluorescent microscopy. -Indirect: serology: Complement fixation or cold agglutinin test. Treatment: -Macrolide. -Resistance strains; Tetracycline or Fluoroquinolones.