http://vilenski.org/science/safari/cellstructure/chloroplasts.html PHOTOSYNTHESIS REVIEW http://www.science.siu.edu/plant-biology/PLB117/JPEGs%20CD/0076.JPG NAME THE MOLECULES 5 carbon CO2 acceptor that combines with CO2 in the first step of the Calvin cycle ________________________________ Ribulose biphosphate (RuBP) Enzyme that catalyzes the addition of CO2 to RuBP RuBP carboxylase (RUBISCO) ________________________ 3 carbon sugar produced during the Calvin cycle that can be used to build glucose and other organic molecules Glyceraldehyde-3-phosphate (G3P) ______________________________ To make one glucose molecule, the Calvin cycle uses 6 molecules of CO2 _____ 18 molecules of ATP _____ 12 molecules of NADPH _____ Campbell concept check 10.3 NAME THE MOLECULES Enzyme that adds CO2 to make a 4 carbon molecule before entering the Calvin cycle in C4 and CAM plants ________________________________ PEP carboxylase Cluster of proteins in the thylakoid membrane that uses the energy of a hydrogen ion gradient to add an inorganic phosphate group to ADP ________________________ ATP synthase Iron containing proteins that make up the electron transport chain cytochromes ______________________________ NAME THE MOLECULES Molecule that is split during the light reaction to supply H+ ions for the concentration gradient and electrons to replace those lost by chlorophyll ________________________________ water Yellow and orange pigment molecules that broaden the spectrum of colors used to drive photosynthesis and also protect chlorophyll by dissipating excess light energy carotenoids _________________________ NAME THE COFACTOR found in the cytochromes of the electron transport chain Fe (iron) _____ NAME THE COFACTOR found in the center ++ (magnesium) Mg chlorophyll molecule _____ Which color of light is least effective in driving photosynthesis? Campbell Concept check 10.2 Green; green light is reflected not absorbed Name the chlorophyll molecules found in the reaction center of Photosystem II. Chlorophyll a (P680) Explain why chlorophyll excited by light will fluoresce and give off heat when removed from cell, but not in intact chloroplasts. Excited electrons can’t pass their energy on to the electron transport chain if removed from chloroplasts. Energy is given off as heat and light (fluorecence) instead http://plantsinmotion.bio.indiana.edu/usbg/photosyn.htm Name the chlorophyll molecules found in the reaction center of Photosystem I. Chlorophyll b (P700) What is the purpose of cyclic electron flow? Non-cyclic electron flow produces equal amounts of ATP and NADPH, but Calvin cycle requires more ATP than NADPH. Cyclic electron flow can make up the difference by making more ATP without making NADPH. Cell can switch to cyclic electron flow, if NADPH is high and cell needs more ATP to run Calvin cycle Instrument that measures the proportions of light of different wavelengths absorbed and transmitted by a pigment solution spectrophotometer Energy coupling mechanism that uses energy stored in the form of a hydrogen gradient across a membrane to drive cellular work, like the synthesis of ATP chemiosmosis NAME THE PARTS Gel-filled space outside the chloroplast cytoplasm = __________________ Gel-filled space outside the stacks = ______________ stroma Stack of sacs = __________ granum MEMBRANE SAC thylakoid = _________________ Gel-filled space inside the membrane sac Thylakoid space = _________________ Water (H2O) #1 = ______________ Oxygen (O2) #2 = ______________ Carbon dioxide (CO2) #3 = ______________ Carbohydrates #4 = ______________ Sunlight (light energy) #5 = ______________ Name the 2 kinds of plants that use alternative methods of carbon fixation which joins C02 to make a 4 carbon molecule before entering the Calvin cycle C4 and CAM What does CAM stand for? Crassulacean acid metabolism In which types of climates would you expect to find more of these plants? Hot, dry regions Tell the place where the enzymes for the light reactions are located In membranes of thylakoids Tell the place where the Calvin cycle happens stroma Tell the place H+ ions build up as electrons move down the electron transport chain In thylakoid space inside sac Tell the place where ATP is made stroma pigment A _________________ is a molecule (like chlorophyll) that absorbs light energy. Name the two types of chlorophyll Chlorophyll a & _________________ Chlorophyll b ________________ Name the cells unique to C4 plants where the Calvin cycle is found Bundle sheath cells Name the two stages of photosynthesis Light reactions & Calvin cycle In which of these are carbohydrates produced? Calvin cycle The Calvin cycle is also referred to as the ____________________ reactions Light independent Name the carrier of high energy electrons and hydrogen used during photosynthesis NADP+ In which stage of photosynthesis is ATP made? Light dependent reactions In which phase of photosynthesis is oxygen released to the atmosphere? Light reactions What is the ultimate fate of the H in H2O during photosynthesis? Ends up in carbohydrates ATP synthase uses the movement of which ion across the membrane to produce ATP? H+ What is the ultimate fate of the oxygen atoms in H2O following photosynthesis? Released into the atmosphere as O2 gas Write the overall equation for photosynthesis. 6 CO2 + 6 H2O + light energy →CH 6 12O6 + 6 O2 From which reactant do the Oxygen atoms in glucose (C6H12O6) come? Carbon dioxide Where do the electrons that pass down the electron transport chain end up following non-cyclic electron flow? In NADPH Name the products of the light reaction. Oxygen, ATP, NADPH Name the products of the Calvin cycle G3P (carbohydrates) , CO2 In the light reactions, what is the electron donor? Campbell Concept check 10.2 water Where do the electrons end up? NADP+ Campbell Concept check 10.2 Explain why a poison that inhibits an enzyme in the Calvin cycle will also inhibit the light reactions. Campbell Concept check 10.3 Light reactions require ADP and NADP+ which are regenerated from the Calvin cycle. If these don’t return from Calvin Cycle . . . ATP and NADPH production stops Explain when photorespiration would happen in a plant instead of photosynthesis. When it is hot & dry the plant closes its stomata to conserve water; when CO2 levels get low the plant switches to photorespiration How is photorespiration different from photosynthesis? Rubisco adds O2 to Calvin cycle instead of CO2 ; CO2 is given off, but no ATP is made; no sugar is made In C4 plants the steps of carbon fixation and Calvin cycle are separated by location in different types of cells. How are these separated in CAM plants? By time; carbon fixation happens at night, then Calvin cycle uses the stored carbon during the day How many G3P molecules are needed to make 1 glucose molecule? 2 Glyceraldehyde-3-phosphate G3P stands for __________________________ Give an example of a C3 plant Rice, wheat, soybeans, most flowers & trees you know Which of the following is a chlorophyll molecule? Chlorophyll phospholipid amino acid (phenylalanine) glucose ATP Organisms that can produce their own food are called __________________ Autotrophs (producers) RuBP stands for __________________________ Ribulose biphosphate Give an example of a C4 plant Sugarcane, corn Organisms that can’t produce their own food and must get their energy from eating other organisms are called __________________ heterotrophs (consumers) Which of the following sequences correctly represents the flow of electrons during photosynthesis? A) NADPH → O2→CO2 B) H2O →NADPH→ Calvin cycle C) NADPH→chlorophyll→ Calvin cycle D) H2O→photosystem I→photosystem II E) NADPH→ electron transport chain → O2 → Campbell Self quiz The most abundant protein in chloroplasts and probably the most abundant protein rubisco on Earth is ____________________ Which wavelengths of light does chlorophyll absorb best? Red & blue violet The entire range of electromagnetic radiation from microwaves to radiowaves is called Electromagnetic spectrum the __________________________ Chloroplasts are found mainly in which kind of cells in C3 plants? __________________ mesophyll Which of the following sequences correctly represents the flow of electrons during photosynthesis? A) NADPH → O2→CO2 B) H2O →NADPH→ Calvin cycle C) NADPH→chlorophyll→ Calvin cycle D) H2O→photosystem I→photosystem II E) NADPH→ electron transport chain → O2 → Campbell Self quiz