BioScholars Assessment 2015-2016 Michael J. Hanophy St. Joseph’s College, Brooklyn NY PROJECT: Activating prior knowledge to improve learning Fall Semester 2015 COURSE: BIO 151 – General Biology I TOPIC: Cell structure and function Students have learned about cell structure multiple times during their academic career (in the state of New York it appears in the 4th grade and 7th grade curricula as well as in the commencement level biology course referred to in the state as “Living Environment.” The Campbell Biology text stresses the interaction of cellular organelles in the endomembrane system, the movement of materials into and through the cell, and role of the cytoskeleton in cellular organization; all of these are new concepts or at least a more sophisticated level of understanding for my students in freshmen biology. It was believed that carefully reviewing the prior knowledge using diagrams and questions that were familiar to the students, students would be more capable of learning the new or more advanced concepts. FORMATIVE ASSIGNMENTS Pre-quiz 1 (administered via Blackboard) Pre-quiz 2 (administered via Blackboard) Formative Assessment A (administered on Day 2 using Socrative) _____ 1. One difference between prokaryotic and eukaryotic cells is that only eukaryotic cells possess (A) membrane-bound organelles (B) DNA (C) ribosomes (D) cytoplasm _____ 2. Many of the organelles in cells are studied together as part of the endomembrane system because (A) all of the organelles that make up this system are produced in the nucleolus (B) the membranes of these organelles are made of a phospholipid bilayer while other organelles are made of only a monolayer (C) the organelles in this system are connected structurally or by the transfer of membrane vesicles (D) together, the organelles of this system are responsible for all of the dehydration synthesis and hydrolysis that occurs in the cell _____ 3. Pores in the nuclear envelop are necessary to permit the transport of (A) DNA into the endoplasmic reticulum (B) RNA into the endoplasmic reticulum (C) large polysaccharide molecules into the nucleus (D) membrane vesicles into the nucleus Formative Assessment B (administered on Day 3 using Socrative) _____ 1. Evidence to suggest that the chloroplast is descended from an organism that once lived independently includes the fact that (A) chloroplasts are the only organelles capable of directing photosynthesis (B) chloroplasts are a major part of the endomembrane system (C) there is a separate Golgi apparatus inside of most chloroplasts (D) chloroplasts contain their own DNA and ribosomes _____ 2. Integral proteins are unlikely to pop out of a membrane because (A) they form a layer between the layers of phospholipids (B) they are chemically bound to the phospholipids (C) they are folded so that hydrophobic amino acids are exposed on the inside of the membrane and hydrophilic enzymes are exposed on the inner and outer surfaces (D) most of these proteins are connected to the ER with microtubules _____ 3. Which of the following statements about a lipid bilayer is true? (A) The lipid composition of the inner and outer layer is always the same. (B) It is easy for phospholipid molecules to flip from one layer to the other. (C) The position of proteins in a lipid bilayer is determined by where they start out when they are produced on the ER membrane. (D) Proteins move in and out of the bilayer freely. Prior-Knowledge Activity A (pair/share) What cellular structures are visible in this image of a human cheek cells? What does this image tell us about the differences between prokaryotic and eukaryotic cells? What cellular structures are visible in this image of Elodea cells? Note that the nucleolus is more obvious in some of these cheek cells than others. Now that you know the function of the nucleolus, can you explain this? Prior-Knowledge Activity B (pair/share) Note the similarities and differences between the mitochondria on the left and the chloroplast on the right. Chlorophyll and other pigments are intramembrane proteins. So are many of the proteins involved in respiration. How do these facts help explain the structure of the mitochondria and the chloroplast? https://youtu.be/BB5rvjZzgFU cytoplasmic streaming in Elodea What did this early photomicrograph tell researchers about the structure of biological membranes? https://youtu.be/PsYpngBG394 pseudopods in Amoeba How is the cytoskeleton involved in these two cellular processes? (A) Prior-Knowledge Activity C (pair/share) (B) (C) What is different about each of the sections of membrane shown above? How might the difference between A and B affect the physical properties of the membranes? Speculate on the nature of the small molecules present in C that are not present in A or B. Are they hydrophobic or hydrophilic? How might they affect the physical properties of the membrane? ANALYSIS: 16 of 42 multiple choice questions on Examination #1 involved cell structure and function. Table 1 indicates that students did about as well on the multiple choice questions (65.3%) as they did on the overall exam (64%) however 61.3% of the students fared better in the multiple choice section than they did in the overall exam. In an analysis of the 12 MC questions that pertained to cell structure and function, students did not do as well (61%) and only 38.7% of them. TABLE 1 – Comparison of score on multiple MC questions and MC question regarding cells with overall score Analysis of Exam 1 Exam 1 Curved Grade 69 Actual Grade 64 Analysis of ALL MULTIPLE CHOICE questions (42) 65.3 Analysis of Multiple Choice questions involving the CELL (16 of 42) 61 Analysis of Exam Essays 57.1 Better 19.0 61.3% 12.0 38.7% 11.0 35.5% Worse 11.0 35.5% 18.0 58.1% 19.0 61.3% Table 2 compares grades on the first exam over the past several years. The 2015 group had a better average than 2 of 3 of the previous groups and did significantly better in the multiple choice section than any of the groups in the last three years. TABLE 2 – Comparison of overall scores and scores on multiple choice questions over the last 4 years Comparison of Exam 1 grades over past several years #STUDENTS Improvement in Multiple Choice questions EXAM #1 Recorded Grade Actual Grade 2015 2014 2013 2012 2011 31 53 36 62 46 69 63 66 67 69 64 59.2 61.0 64.4 64.9 AVERAGE: 45.5 66.25 62.151 Essay MC 57.1 59.2 58.9 66.4 67.6 59.2 62.5 64.1 67 8.3 points less than 2015 5.0 points less than 2015 3.4 points less than 2015 60.4 63.4 5.6 69.4% 72% MEDIAN AVERAGE 63% 35.8 54.2% 26.8 66% 37.5 56.8% 29.8 67% 74.4% -8.78 3.99 7 8 7.1 8.3 6 7 ESSAY 4 GENETICS ESSAY 3 RESPIRATION/PHOTOSYNTHESIS ESSAY 2 - CELL ESSAY 1 - BIOCHEM SCORE ON ESSAY QUESTIONS Comparison of score on essays to overall exam grade SCORE ON MULTIPLE CHOICE Comparison of score on MC questions to overall exam grade PRECURVE Analysis of Final Exam FINAL 9 of 63 questions on the Final Exam involved cell structure and function. Student performance on the MC questions concerning cell structure and function was very similar to overall performance on MC questions. Students did, however, do better on the essay question concerning cell structure and function than on any of the other essay questions on the final exam. 6.8 9 COMPARISON OF MULTIPLE CHOICE QUESTIONS CONCERNING CELL STRUCTURE AND FUNCTION 5.12 5 56.8% 55.6% CONCLUSIONS: While there appeared to be no immediate improvement in student performance on questions concerning cell structure and function after implementation of the new program, performance on the first exam was better this year than in past years. Also, student performance on the final exam essay concerning cell structure and function was better than on any other of the other essays, indicating the possibility that there might have been some long-term benefit to the new program. In the second semester, new lessons will be prepared for BIO 340 – Microbiology.