Biochemistry and Cell Structure 2-3 Carbon Compounds The Chemistry of Carbon Organic Chemistry – the study of all compounds that contain carbon atoms All organic compounds contain Carbon 3 Ways Carbon Atoms Bond 1. Straight Chain 2. Branched Chain 3. Rings Organic Compounds 4 Types of Organic Compounds Essential to Living Things. 1. Carbohydrates composed of C, H, O 1:2:1 ratio of C:H:O Body breaks down sugars to use as energy for cell activity 3 Types of Carbohydrates A. Monosaccharide Simple sugars Made up of one sugar molecule Ex. Glucose, fructose, galactose B. Disaccharide Double sugar – two monosaccharide molecules linked together Ex. Sucrose (table sugar) C. Polysaccharide The largest and composed of many monosaccharide molecules Ex. Glycogen (in humans) Cellulose & starch (used as food storage by plants) 2. Lipids Fatty acids Large proportion of C, H compared to O Lipids are used for: energy storage insulation protective coatings key component of cell membrane Common categories of lipids are A. Triglycerides Fats – solid at room temperature Oils – liquid at room temperature B. Wax 1 Used for protection by your ear to keep gunk out Used by plants as waterproofing protection C. Steroids These are considered lipids because they can’t combine with water. 3. Nucleic Acid A complex molecule that stores cellular information in the form of a code Nucleotides – make up nucleic acid Nucleic acids store and transmit hereditary, or genetic information Two Kinds of Nucleic Acids RNA – ribonucleic acid Contains sugar ribose DNA – deoxyribonucleic acid Contains sugar deoxyribose 4. Proteins Made up of C, H, O, N Essential to life Provide structure for tissues and organs Carry out cell metabolism Amino Acids – basic building blocks of proteins Proteins are Important in… Contracting muscle tissue Transporting oxygen in blood Providing immunity, Carry out chemical reactions Digestion of food 2-4 Chemical Reactions and Enzymes Chemical Reactions Chemical Reaction – a process that changes one set of chemicals into another set of chemicals Reactants – elements or compounds that enter a chemical reaction Products – elements or compounds produced by a chemical reaction Activation Energy – the energy that is needed to get a reaction started Enzymes Type of catalyst Protein that speeds up a chemical reaction Lowers the amount of activation energy needed Lowering the activation energy effects how fast the reaction is completed. The Induced Fit Model Enzymes are specific to what substrate they will react with The substrate fits into the enzyme like a lock and a key 2 The active site on the enzyme and the shape of the substrate are not a perfect match When they fit together they “mold” to each other Once the reaction is over, the substrate is released The enzyme returns to the original shape and reacts with another substrate Regulation of Enzyme Activity What can affect how enzymes work? pH if pH is to acidic or basic the enzyme will not work most enzymes work best at 7 (neutral) Temperature If it is to hot or cold the enzyme will not work Denature – enzymes active site changes shape and will not bind to the substrate, so no reaction Enzymes play essential roles in Regulating chemical reactions Make materials that cells need Releasing energy Transferring information 7-1 Life Is Cellular The Discovery of Cells Cells are the basic units of life Your body is made of 75 – 100 trillion cells Robert Hooke – 1665 Used one of the first microscopes to view a slice of cork He saw that cork is made up of lots of little boxes He named them cells Anton van Leeuwenhoek – 1675 Used a microscope to view a drop of pond water He saw “critters” living in the water Schleiden Said that all plants are made entirely of cells Schwann Said that all animals are made up entirely of cells Virchow Said that all cells come from existing cells The Cell Theory There are 3 parts to the Cell Theory 1. All living things are made of cells 2. Cells are the basic units of structure and function 3 3. Cells are produced from existing cells 2 Types of Cells 1. Prokaryotes Pro means before Karyote means nucleus A cell that does not contain a nucleus DNA is free floating inside the cell Do not have membrane bound organelles Most are single-celled organisms (unicellular) First appeared about 2 billion years ago Ex. Bacteria 2. Eukaryote Eu means true A cell that contains a nucleus with DNA inside They have organelles Are either single celled or made of many cells (multicellular) Evolved 1.5 billion years ago Ex. Plants, animals, fungi, protists 7-2 Eukaryotic Cell Structure Two different types of Eukaryotic Cells Plant Cell Animal Cell Organelles – membrane bound structures in a eukaryotic cell that manages the cell’s functions Cell Shape Cytoplasm The clear gelatinous fluid inside a cell Surrounds all of the organelles Cell Membrane Thin, flexible barrier around a cell Regulates what enters and leaves the cell Cell Wall A rigid structure located outside the plasma membrane that provides additional support and protection Occurs in plant cells, fungi, most bacteria and some protists. Protein Production Nucleus cells largest organelle Is surrounded by a double membrane called the nuclear envelope Contains DNA – directions to make proteins 4 Nucleolus Organelle within the nucleus Makes ribosomes Ribosomes Ribosomes move out of nucleus Where proteins are made Assembly, Transport and Storage Endoplasmic Reticulum (ER) Site of cellular chemical reactions A series of folded membranes Transports proteins and other materials to the golgi apparatus Two types of ER 1. Rough ER Covered with ribosomes Makes proteins 2. Smooth ER Contains no ribosomes Makes lipids Golgi apparatus “Packaging and distribution” center of cell Sorts proteins into packages (vesicles) to be sent to the appropriate destination in the cell or outside of the cell Vacuoles Temporary storage of materials like food, enzymes and other materials needed by the cell and cell waste In plant cells there is one very large vacuole In animal cells there are many small vacuoles Lysosomes Small organelles filled with enzymes Enzymes break down lipids, carbohydrates, worn out organelles, viruses and bacteria. “recycling centers” Plastids Only in plants Some are used to store starches, lipids and others contain pigments to give them color Energy Transformers Mitochondria Powerhouse of the cell make energy for the cell Chloroplasts Type of plastid Only in green plants and some protists Capture light energy and produce food 5 Necessary for photosynthesis Contain Chlorophyll - green pigment Structures for Support and Locomotion Cytoskeleton Give cells support structure Composed of tiny rods called microtubules and filaments called microfilaments Centrioles Microtubules that help with cell division Cilia Short numerous hairlike projections Move in a wavelike motion to help cell move Flagella Long tail like projection Move with a whiplike motion Difference between Plant and Animal Cells Plant Cell Animal Cell photosynthesis no photosynthesis Contain chloroplasts no chloroplasts Contain chlorophyll no chlorophyll Cell wall no cell wall One large vacuole many small vacuoles Square round In general larger smaller 6