Student Lab Microscope Measurement Software SOP
Page 1 of 6
Revision 1-030110
Student Lab Microscope Measurement/Image
Capture Software SOP
1. Scope
1.1
This document provides the operating procedures for the QCapture Pro measurement/image capture
software and the student lab microscope.
2. Table of Contents
1. Scope ............................................................................................................................................................. 1
2. Table of Contents .......................................................................................................................................... 1
3. Reference Documents ................................................................................................................................... 2
3.1
Referenced within this Document .......................................................................................................... 2
3.2
External Documents ............................................................................................................................... 2
4. Equipment and/or Materials ......................................................................................................................... 2
5. Safety............................................................................................................................................................. 2
Student Lab Microscope Measurement Software SOP
Page 2 of 6
Revision 1-030110
6. Setup Procedures .......................................................................................................................................... 2
6.1
Setup Microscope ................................................................................................................................... 2
6.2
Open QCapture Software ....................................................................................................................... 3
7. Capture and Measurement Procedures........................................................................................................ 3
7.1
View and Capture Images ....................................................................................................................... 3
7.2
Save Captured Images ............................................................................................................................ 4
7.3
Measure Dimensions on Captured Images............................................................................................. 4
7.4
Add a Scale Bar ....................................................................................................................................... 5
8. Shutdown Procedure .................................................................................................................................... 5
8.1
Exit Q-Capture ........................................................................................................................................ 5
8.2
Turn off Microscope ............................................................................................................................... 5
9. Revision History............................................................................................................................................. 6
Figure 1, Microscope Controls ................................................................................................................................... 3
Figure 2, Optic Power Supply ..................................................................................................................................... 3
Figure 3, Camera Icon Shortcut .................................................................................................................................. 3
Figure 4, Digital Camera Control Windows ................................................................................................................ 4
Figure 5, Calibration Window..................................................................................................................................... 4
Figure 6, QCapture Workspace .................................................................................................................................. 5
3. Reference Documents
3.1
Referenced within this Document
3.1.1
3.2
None
External Documents
3.2.1
QCapture-Pro Manual v1.3
4. Equipment and/or Materials
4.1
Microscope
4.2
Wafer/Sample
4.3
QCapture Pro Software
5. Safety
5.1
Follow all Nanofab safety procedures.
6. Setup Procedures
6.1
Setup Microscope
6.1.1
Turn on microscope power. See Figure 1, Microscope Controls.
6.1.2
Rotate objectives to lowest objective.
Student Lab Microscope Measurement Software SOP
Page 3 of 6
Revision 1-030110
Z Knob
XY Knob
Power Switch
Figure 1, Microscope Controls
6.1.3
Place sample on microscope stage.
6.1.4
Use the XY knob to center sample under microscope objective. See Figure 1.
6.1.5
Rotate objectives to desired magnification.
Voltage
Knob
Figure 2, Optic Power Supply
6.1.6
Use the Voltage knob on the optic power supply to adjust the light intensity, as needed. See
Figure 2, Optic Power Supply.
6.1.7
Use the Z knob to focus the image. See Figure 1, Microscope Controls.
6.2
Open QCapture Software
6.2.1
Double-click the QCapture Pro icon on the desktop.
7. Capture and Measurement Procedures
7.1
View and Capture Images
7.1.1
Click on the camera icon shortcut. See Figure 3, Camera Icon Shortcut.
NOTE: This will bring up the Digital Camera control
window.
Figure 3, Camera Icon Shortcut
Student Lab Microscope Measurement Software SOP
Page 4 of 6
Revision 1-030110
Basic Dialog Box
Preview
Advanced View
Snap
Calc
Auto
Exp
Basic
Dialog
Figure 4, Digital Camera Control Windows
7.1.2
If not already done, switch to the Basic Dialog view. See Figure 4.
7.1.2.1
In the Advanced view, click Basic Dialog. See Figure 4.
7.1.3
Click PREVIEW to see a live image from the camera. See Figure 4.
7.1.4
Click CALC AUTO EXP to adjust camera lighting. See Figure 4.
7.1.5
Click SNAP to grab the image. See Figure 4.
7.2
Save Captured Images
7.2.1
Click on the captured image.
7.2.2
Select File>Save As to save a jpeg file in your directory.
7.3
Measure Dimensions on Captured Images
7.3.1
Capture an image, See 7.1, or Open an image from a file.
7.3.2
Click on the calibrate icon shortcut (calipers). See Figure 6, QCapture Workspace.
7.3.3
Select the correct magnification from the drop down menu that matches the objective. See
Figure 5, Calibration Window.
Drop-down
Menu
Figure 5, Calibration Window
Student Lab Microscope Measurement Software SOP
Page 5 of 6
Revision 1-030110
7.3.4
Click on the measure icon shortcut (compass and ruler). See Figure 6, QCapture Workspace.
7.3.5
Click on the image to select the first point, drag to the second point, and release.
7.4
Add a Scale Bar
7.4.1
In the Calibration dialog box, click Marker. See Figure 6.
7.4.2
Choose the style of scale bar.
7.4.3
Adjust the location of the scale bar.
8. Shutdown Procedure
8.1
Exit Q-Capture
8.1.1
8.2
Close all windows and exit the program when not in use.
Turn off Microscope
8.2.1
Turn off light source.
8.2.2
Rotate objectives to lowest magnification.
8.2.3
Unload wafer/sample.
Camera Icon
Calibrate
Measure
Basic
Dialog
Box
Captured
Image
Measurement
Measure
Distance
Dialog Box
Calibration
Dialog Box
Figure 6, QCapture Workspace
Student Lab Microscope Measurement Software SOP
Revision 1-030110
9. Revision History
Rev
Date
1
01 Mar 2010
Originator
Sam Bell
Description of Changes
Page 6 of 6