Genetics Notes 3 Test Cross How do we know if an organism that shows a dominant trait has a two dominant alleles or one dominant and one recessive allele? • A _________________ is a method for determining whether an organism that ___________________ __________________________________________ _________________________________________. Test Cross • In a test cross, _________________________ _____________________________________. – If the test organism is heterozygous, ___________ ________________________________________. – If the test organism is homozygous dominant, ________________________________________. – Large numbers of offspring are necessary for valid results. Test Cross Example: • In guinea pigs, black coat color is dominant over white. How would you find out whether a black guinea pig is homozygous for coat color (BB) of heterozygous (Bb)? – _________________________________________ _________________________________________ ________________________________________. Test Cross • Using the Punnett Squares below, show the test cross described above: Multiple Alleles • So far, we have discussed traits that have only two possible alleles (Ex. Yellow/Green; Tall/Short; Round/Wrinkled), but for many genes, several alleles exist in the population. – This is called _______________________ – This expands the number of possible _____________________________ Multiple Alleles Example: Multiple alleles control the character of ____________________ in humans. A person may be ______, ______, ______, or ______. • The letters refer to _______________________, called A and B, which are _____________________________ _______________________ – A persons red blood cells may be coated with _____ _____________ (A or B), ___________ (AB), or _____________ (O) • The alleles are written as • IA = _____ • IB = _____ • i = ______ Multiple Alleles • Each person inherits ___________________________. • There are 6 possible ways to inherit the alleles: – – – – – _______ _______ _______ _______ _______ • O (i allele) is _________________ • A and B (IA and IB alleles) are called ________________: ______________________________ – Both carbohydrate A and B are present on red blood cells Multiple Alleles • How is this different from incomplete dominance? – ___________________________________ ___________________________________ • Ex. ______________________________ – ___________________________________ Genotype ii IAIA IAi IBIB IBi IAIB Phenotype Review: Dihybrid Cross • Using the Punnett square below, work out the following cross: TTYY x TtYy Review: Dihybrid Cross • Using the Punnett square, work out the following cross: rrYy x RRyy Review: Blended Inheritance (Incomplete Dominance) Example: • Crossing pure red (RR) and pure white (WW) Japanese four-o’clock flowers results in an F1 generation in which all of the flowers are pink (RW). Show a cross between a pure red flower (RR) and a pure white flower (WW). Phenotypes:_________ Review: Blended Inheritance (Incomplete Dominance) Show a cross between two members of the F1 generation (RW x RW). Phenotypes:_________ Review: • What is Mendel’s law of independent assortment? • What is incomplete dominance? • What is codominance? • When do you use a test cross? • When do you use a dihybrid cross? • When do you use a monohybrid cross?