Cal-OSHA 5209 LISTED CARCINOGENS
STANDARD OPERATING PROCEDURE TEMPLATE
Type of SOP:
Process
Hazardous Chemical
Hazard Class
OVERVIEW
This SOP deals with the carcinogens listed in the Cal-OSHA regulation Title 8,
Subchapter 7, Group 16, Article 100, section 5209:
Chemical Name
2-Acetylaminofluorene
4-Aminobiphenyl
Benzidine (and its salts)
3,3’-Dichlorobenzidine (and its salts)
4-Dimethylaminoazobenzene
-Naphthylamine
-Naphthylamine
4-Nitrobiphenyl
N-Nitrosodimethylamine
-Propiolactone
Bis-Chloromethyl ether
Methyl chloromethyl ether
Ethyleneimine (aziridine)
CAS Number
53-93-6
92-67-1
92-87-5
91-94-1
60-11-7
134-32-7
91-59-8
92-93-3
62-75-9
57-57-8
542-88-1
107-30-2
151-56-4
These 13 entities are the most strictly regulated chemicals addressed by Cal-OSHA and
OSHA. A majority of the regulatory requirements described for these chemicals do not
apply to laboratory use, as the laboratory use of chemicals is instead covered in Title 8,
Subchapter 7, Group 16, Article 109, section 5191, commonly referred to as the
Laboratory Standard. The laboratory standard overrides most other regulations, with
the exception of the following:





The requirement to limit employee exposure to the specific exposure limit for a
chemical.
When that particular regulation states otherwise, as in the case of Section
5209(c)(6). – see below
Prohibition or prevention of eye and skin contact.
When the action level is exceeded as determined by exposure monitoring.
‘Report of use’ requirements (EH&S function)
As such, the requirements described in 5209(c)(6) must be complied with, as described
below.
5209(c)(6): Requirements applying to research and quality control activities
involving the use of the above listed carcinogens:











Mechanical pipetting aids shall be used for all pipetting procedures.
Experiments, procedures, and equipment which could produce aerosols shall be
confined to laboratory type hoods or glove boxes. Hoods must maintain an
average airflow of 150 fpm. (campus hoods are normally at 100 fpm, so 150 fpm
requires EH&S coordination/approval).
Laboratory work surfaces on which these carcinogens are handled shall be
protected from contamination.
Contaminated wastes and animal carcasses shall be collected in impervious
containers which are closed and decontaminated prior to removal from the work
area. Such wastes and carcasses shall be incinerated in such a manner that no
carcinogenic products are released.
All other forms of these carcinogens shall be inactivated prior to disposal.
Laboratory vacuum systems shall be protected with high efficiency scrubbers or
with disposable absolute filters except that only high efficiency scrubbers shall be
used with -propiolactone, bis-chloromethyl ether, methyl chloromethyl ether, or
ethyleneimine.
Employees shall be provided with, and shall be required to wear, a daily change
of clean, protective laboratory clothing, such as a solid-front gown, surgical scrub
suit or lab coat.
Prior to each exit from a regulated area, employees shall be required to remove
and leave protective clothing and equipment at the point of exit and, at the last
exit of the day, to place used clothing and equipment in impervious containers at
the point of exit for decontamination/disposal. Containers shall be labeled as to
their contents.
Employees shall be required to wash hands, forearms, face and neck upon each
exit from the regulated area.
A current inventory of these carcinogens shall be maintained
Lab hoods and other ventilation equipment shall be tested at least semi-annually.
Labelling
Reproductive hazards and carcinogens are now identified by the United Nations
Globally Harmonized System of Classification and Labeling of Chemicals (GHS).
Newer chemical containers manufactured after the implementation of GHS will have this
symbol
Respiratory sensitization; germ cell mutagenicity; carcinogenicity
reproductive toxicity; specific target organ toxicity; aspiration hazard.
Secondary and non-manufacturer containers of these carcinogens must be properly
labelled with the identity of the material and the relevant hazards. Labels can be
generated using the UC MSDS database at:
http://jr.chemwatch.net/chemwatch.web/dashboard
WORKING WITH 5209 regulated carcinogens
All of the above materials have in common the fact that direct contact must be avoided.
Careful handling and stringent controls are essential in order to minimize risk to
researchers and the environment. Therefore, in addition to the 5209-specific
requirements listed above, the requirements outlined in the following sections must be
followed rigorously.
Note that this standard operating procedure describes the baseline requirements
for handling these classes of compounds. Additional information about working with
hazardous chemicals can be found in Prudent Practices in the Laboratory, National
Research Council, 2011 Chapters 4-6.
PERSONAL PROTECTIVE EQUIPMENT (PPE)
See the PPE information under Sec. II of the UCSB Chemical Hygiene Plan regarding:
 the UC PPE Policy and policy summary (what PPE is needed and when/where to
use)
 obtaining your PPE via use of the Laboratory Hazard Assessment Tool
 glove selection criteria
 respirator use, etc.
At minimum, complete protection of the eyes and skin is essential.
ENGINEERING/VENTILATION CONTROLS
These substances should always be used in a fume hood, glove box, or in totally-sealed
containers. Fume hoods must be specially designated for use of these carcinogens,
and maintain an average flowrate of 150 fpm. For further information see the following
pages in Sec. II of the UCSB Chemical Hygiene Plan:
 Fume Hood Usage Guide

Criteria for Implementing Engineering Controls
SPECIAL HANDLING PROCEDURES
 Never work alone with extremely hazardous materials.
 Eliminate or substitute for a less hazardous material when possible.
 Design your experiment to use the least amount of material possible to achieve
the desired result.
 Do not exceed the scale or deviate from the experimental parameters which may
be outlined in the lab-specific information section below without the approval of
the PI.
 Perform adequate hazard analysis and risk assessment before beginning the
experiment, as described in in Prudent Practices in the Laboratory, National
Research Council, 2011 Chapters 4-6.
STORAGE REQUIREMENTS
All hazardous materials must be labeled with their identity as well as all applicable
warning statements. Manufacturer labels will contain all the necessary information.
However, if material is repackaged or synthesized in the laboratory, please follow the
protocols described in the CHEMICAL LABELLING section in Section II of the UCSB
Chemical Hygiene Plan.
SPILL AND INCIDENT PROCEDURES
See directions under the “Chemical Incident” tab of the UCSB Emergency Information
Flipchart – should already be posted in all labs.
DECONTAMINATION
Using proper personal protective equipment as outlined above, decontaminate
equipment and bench tops using soap and water and properly dispose of all chemical
and contaminated disposables as hazardous waste following the guidelines below.
WASTE DISPOSAL
See “Chemical Waste Disposal” in Sec. II of the UCSB Chemical Hygiene Plan.
PRIOR APPROVAL/REVIEW REQUIRED
As they deem necessary, the PI/supervisor should insert here any prior approval or
review needed before an individual can do the operation.
DESIGNATED AREA
As they deem necessary, the PI/supervisor should insert here any information about
whether a special use-area is designated for this material/process.
SAFETY DATA SHEETS
Found online at: http://ehs.ucsb.edu/labsafety/msds
LAB-SPECIFIC INFORMATION (required)
Add appropriate lab-specific information here describing how this material(s) is
generally used. E.g., name of protocol, frequency done, scale, temperature, etc.